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  4. Recombinant Arabidopsis thaliana Lysine-specific demethylase REF6 (REF6), partial

Recombinant Arabidopsis thaliana Lysine-specific demethylase REF6 (REF6), partial

  • 中文名稱：
    擬南芥REF6重組蛋白
  • 貨號：
    CSB-YP871158DOA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥REF6重組蛋白
  • 貨號：
    CSB-EP871158DOA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥REF6重組蛋白
  • 貨號：
    CSB-EP871158DOA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥REF6重組蛋白
  • 貨號：
    CSB-BP871158DOA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥REF6重組蛋白
  • 貨號：
    CSB-MP871158DOA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    REF6
  • Uniprot No.：
  • 別名：
    REF6; JMJ12; PKDM9A; At3g48430; T29H11_50; Lysine-specific demethylase REF6; EC 1.14.11.-; Jumonji domain-containing protein 12; Lysine-specific histone demethylase REF6; Protein RELATIVE OF EARLY FLOWERING 6
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Histone demethylase that demethylates 'Lys-27' (H3K27me) of histone H3. Demethylates both tri- (H3K27me3) and di-methylated (H3K27me2) H3K27me. Demethylates also H3K4me3/2 and H3K36me3/2 in an in vitro assay. Involved in the transcriptional regulation of hundreds of genes regulating developmental patterning and responses to various stimuli. Binds DNA via its four zinc fingers in a sequence-specific manner, 5'-CTCTGYTY-3', to promote the demethylation of H3K27me3 and the regulation of organ boundary formation. Involved in the regulation of flowering time by repressing FLOWERING LOCUS C (FLC) expression. Interacts with the NF-Y complexe to regulate SOC1. Mediates the recruitment of BRM to its target loci.
  • 基因功能參考文獻：
    1. BES1 recruits transcriptional regulators ELF6 and REF6 to regulate target gene expression and coordinate brassinosteroids responses with other developmental processes such as control of flowering time. PMID: 18467490
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    JHDM3 histone demethylase family
  • 組織特異性：
    Highly expressed in the shoot apical meristem and primary and secondary root tips, and lower expression in cotyledons, leaves and root axis along vascular tissues. Detected in inflorescences, stems and siliques.
  • 數據庫鏈接：

    KEGG: ath:AT3G48430

    STRING: 3702.AT3G48430.1

    UniGene: At.3169



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