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  4. Recombinant Arabidopsis thaliana L-type lectin-domain containing receptor kinase I.9 (LECRK19), partial

Recombinant Arabidopsis thaliana L-type lectin-domain containing receptor kinase I.9 (LECRK19), partial

  • 中文名稱：
    擬南芥LECRK19重組蛋白
  • 貨號：
    CSB-YP881802DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥LECRK19重組蛋白
  • 貨號：
    CSB-EP881802DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥LECRK19重組蛋白
  • 貨號：
    CSB-EP881802DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥LECRK19重組蛋白
  • 貨號：
    CSB-BP881802DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥LECRK19重組蛋白
  • 貨號：
    CSB-MP881802DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    LECRK19
  • Uniprot No.：
  • 別名：
    LECRK19; DORN1; P2K1; At5g60300; F15L12.17; L-type lectin-domain containing receptor kinase I.9; LecRK-I.9; LecRK79; EC 2.7.11.1; Protein DOES NOT RESPOND TO NUCLEOTIDES 1; Purinoreceptor kinase 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Essential receptor for extracellular ATP. Binds ATP with high affinity through its extracellular legume-lectin like region. Is required for ATP-induced intracellular calcium response, mitogen-activated protein kinase 3 (MPK3) and MPK6 activation and ATP-induced gene expression. May play a variety of roles in stress resistance. May be involved in protein-protein interactions with RGD motif-containing proteins as potential ligands. Plays probably a structural and signaling role at the plant cell surfaces.; Involved in resistance response to the pathogenic oomycetes Phytophthora infestans and Phytophthora capsici.
  • 基因功能參考文獻：
    1. Mutation of DORN1 phosphorylation sites on RBOHD eliminates the ability of ATP to induce stomatal closure. The data implicate purinergic signaling via DORN1 in the control of stomatal aperture. PMID: 29273780
    2. The role of LecRK-I.9 is discussed with regard to the potential molecular mechanisms linking jasmonic acid signalling to cell wall damage and/or extracellular ATP perception. PMID: 27399963
    3. X-ray crystallographic studies of the extracellular domain of the first plant ATP receptor, DORN1, and the orthologous protein from Camelina sativa, have been presented. PMID: 27710944
    4. the genetic and biochemical evidence for the role of DORN1 in extracellular ATP signalling, placing this within the wider context of extracellular ATP signalling during plant stress responses. [Review] PMID: 25301072
    5. LecRK-I.9 insertion mutants gain susceptibility to Phytophthora. Overexpression enhances resistance. Observations strongly suggest that LecRK-I.9 plays a role in disease resistance by stabilizing the cell wall-plasma membrane continuum. [LecRK-I.9] PMID: 21483488
    6. DORN1 is essential for perception of extracellular ATP and likely plays a variety of roles in plant stress resistance. PMID: 24436418
    7. The lectin receptor kinase encoded by At5g60300 is involved in protein-protein interactions with RGD-containing proteins as potential ligands, and may play a structural and signaling role at the plant cell surfaces. PMID: 16361528

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  • 亞細胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family; Leguminous lectin family
  • 組織特異性：
    Expressed in leaf petioles.
  • 數據庫鏈接：

    KEGG: ath:AT5G60300

    STRING: 3702.AT5G60300.3

    UniGene: At.21114



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