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  4. Recombinant Arabidopsis thaliana Iron-sulfur cluster assembly protein 1 (ISU1)

Recombinant Arabidopsis thaliana Iron-sulfur cluster assembly protein 1 (ISU1)

  • 中文名稱：
    擬南芥ISU1重組蛋白
  • 貨號：
    CSB-YP524352DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥ISU1重組蛋白
  • 貨號：
    CSB-EP524352DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥ISU1重組蛋白
  • 貨號：
    CSB-EP524352DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥ISU1重組蛋白
  • 貨號：
    CSB-BP524352DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥ISU1重組蛋白
  • 貨號：
    CSB-MP524352DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ISU1
  • Uniprot No.：
  • 別名：
    ISU1; ISCU1; At4g22220; T10I14.50; Iron-sulfur cluster assembly protein 1; AtISU1; AtIscU1; Protein ISCU-LIKE 1; NifU-like N-terminal domain-containing protein ISU1; NifU-like protein ISU1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    51-167
  • 氨基酸序列
    PNVGTGLVGA PACGDVMKLQ IKVDEKTGQI VDARFKTFGC GSAIASSSVA TEWVKGKAME DVLTIKNTEI AKHLSLPPVK LHCSMLAEDA IKAAVKDYKE KRVKTNGAAA AGETTQA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins. First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU (ISU1, ISU2 or ISU3). In a second step, the cluster is released from ISCU, transferred to a glutaredoxin, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH.
  • 基因功能參考文獻：
    1. Results suggest that AtHscA2 in cooperation with AtIsu1 and AtHscB play an important role in the regulation of the Fe-S cluster assembly pathway in plant mitochondria. PMID: 25462017
    2. ISU1 has a role in mediating the assembly of iron-sulfur clusters in mitochondria. PMID: 17417719
  • 亞細胞定位：
    Mitochondrion matrix. Cytoplasm, cytosol.
  • 蛋白家族：
    NifU family
  • 組織特異性：
    Expressed in roots, stems, leaves, flowers, pollen and siliques.
  • 數據庫鏈接：

    KEGG: ath:AT4G22220

    STRING: 3702.AT4G22220.1

    UniGene: At.43669



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