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  4. Recombinant Arabidopsis thaliana Histidine kinase 4 (AHK4), partial

Recombinant Arabidopsis thaliana Histidine kinase 4 (AHK4), partial

  • 中文名稱：
    Recombinant Arabidopsis thaliana Histidine kinase 4(AHK4),partial
  • 貨號：
    CSB-YP871664DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Histidine kinase 4(AHK4),partial
  • 貨號：
    CSB-EP871664DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Histidine kinase 4(AHK4),partial
  • 貨號：
    CSB-EP871664DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Histidine kinase 4(AHK4),partial
  • 貨號：
    CSB-BP871664DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Histidine kinase 4(AHK4),partial
  • 貨號：
    CSB-MP871664DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    AHK4
  • Uniprot No.：
  • 別名：
    AHK4; CRE1; RAW1; WOL; At2g01830; T23K3.2Histidine kinase 4; EC 2.7.13.3; Arabidopsis histidine kinase 4; AtHK4; Cytokinin receptor CYTOKININ RESPONSE 1; AtCRE1; Cytokinin receptor CRE1; Phosphoprotein phosphatase AHK4; EC 3.1.3.16; Protein AUTHENTIC HIS-KINASE 4; Protein ROOT AS IN WOL 1; Protein WOODEN LEG
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Cytokinins (CK) receptor related to bacterial two-component regulators. Binds also the synthetic urea-type cytokinin thidiazuron, a potent defoliant and herbicide. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. In the presence of cytokinin, feeds phosphate to phosphorelay-integrating histidine phosphotransfer protein (HPt) and activates subsequent cascade. In the absence of cytokinin, removes phosphate from HPt proteins, decreasing the system phosphoload. Involved in meristems establishment in seedlings. Acts as a redundant negative regulator of drought and salt stress responses, and abscisic acid (ABA) signaling in a cytokinin-dependent manner. Required to set vascular asymmetric cell divisions that establish phloem and procambium cell lines. Redundant positive regulator of cytokinin signaling that regulates many developmental processes including seed germination, cell division, seed size, chlorophyll retention during leaf senescence, root repression and shoot promotion. Can interact with isoprenoid-type cytokinins trans-zeatin (tZ and tZR), isopentenyladenine (iP), and isopentenyladenosine (iPR), the meta hydroxylated derivative of benzyladenine m-topolin, buta-2,3-dienyladenine (HA-8), penta-2,3-dienyladenine (HA-1), 4-methyl-penta-2,3-dienyladenine (HA-10), 4-hydroxy-2-butynyladenine (RM1), 2-butynyladenine (RM6), and to a lower extent, with cis-zeatin (cZ), zeatin riboside and dihydrozeatin (DZ). Together with AHK3, involved in the cytokinin-dependent responses to Pi starvation and sucrose stresses. Required for the formation of auxin-transporting vascular tissues in the hypocotyl, and primary and lateral roots, but not in adventitious roots, thus leading to auxin basipetal transport that regulates root development and branching. Involved in alkamides (e.g. N-isobutyl decanamide) and N-acylethanolamides (NAE) signaling that control meristematic activity and differentiation processes during plant development. Prevents the uptake of sulfate by mediating cytokinin-dependent down-regulation of high-affinity sulfate transporters (e.g. SULTR1;1 and SULTR1;2) expression in roots. Together with AHK2, required for growth and reproduction promotion stimulated by the endophytic fungus Piriformospora indica in a trans-zeatin-dependent manner. Required to trigger the phytotoxic effect of the snapdragon (Antirrhinum majus) flowers volatile organic compound (VOC) methyl benzoate (MB). Plays a role in the cytokinin-mediated repression of the iron uptake pathway.
  • 基因功能參考文獻：
    1. an important role for endogenous cytokinin biosynthesis and AHK4-mediated cytokinin signalling in the control of de novo-induced organ identity. PMID: 29967282
    2. Results show the stress physiology function of histidine kinaseS AHK2, AHK3 AND AHK4. PMID: 25263537
    3. N6-(benzyloxymethyl)adenosine is a novel anticytokinin, an antagonist of cytokinin receptor CRE1/AHK4 of Arabidopsis. PMID: 22773006
    4. Programmed cell death induced by cytokinin is mediated by the cytokinin receptor CRE1. PMID: 22312114
    5. AHK2 and AHK3 signal specifically in leaf parenchyma cells, AHK3 in stomata cells, and CRE1/AHK4 in the root vasculature. CRE1/AHK4 can functionally replace AHK2 but not AHK3. PMID: 21426428
    6. growth and development of the ahk2 ahk3 ahk4 triple mutant were markedly inhibited in various tissues and organs, including the roots and leaves in the vegetative growth phase and the influorescence meristem in the reproductive phase PMID: 15155880
    7. the diverse cytokinin compounds might have specific functions in the numerous cytokinin-regulated processes, which may depend in turn on different receptors and their associated signalling pathways PMID: 15509853
    8. The sugar effect in shoots of phosphate-starved plants was particularly enhanced in the cre1 ahk3 double mutant. PMID: 15923327
    9. findings imply that functional disturbance of AHK4 (a cytokinin receptor) affects other receptors & suggests that cytokinin receptors are needed for formation of auxin-transporting vascular tissues in the hypocotyl, but not in adventitious roots PMID: 16357038
    10. Results reveal partially redundant functions of the cytokinin receptors and prominent roles for the AHK2/AHK3, but not AHK4, receptor combination in quantitative control of organ growth in plants, with opposite regulatory functions in roots and shoots. PMID: 16361392

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  • 亞細胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 組織特異性：
    Mostly expressed in roots, specifically in the vascular cylinder and pericycle, and, to a lower extent, in leaves and flowers. Present in seedlings.
  • 數據庫鏈接：

    KEGG: ath:AT2G01830

    STRING: 3702.AT2G01830.2

    UniGene: At.10485



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