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  4. Recombinant Arabidopsis thaliana Flap endonuclease GEN-like 2 (GEN2), partial

Recombinant Arabidopsis thaliana Flap endonuclease GEN-like 2 (GEN2), partial

  • 中文名稱：
    擬南芥GEN2重組蛋白
  • 貨號：
    CSB-YP878680DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥GEN2重組蛋白
  • 貨號：
    CSB-EP878680DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥GEN2重組蛋白
  • 貨號：
    CSB-EP878680DOA-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥GEN2重組蛋白
  • 貨號：
    CSB-BP878680DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥GEN2重組蛋白
  • 貨號：
    CSB-MP878680DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    GEN2
  • Uniprot No.：
  • 別名：
    GEN2; At3g48900/At3g48910; T21J18.170/T2J13.250; Flap endonuclease GEN-like 2; EC 3.1.-.-
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Endonuclease which cleaves flap structures at the junction between single-stranded DNA and double-stranded DNA with a specific cleavage site in the 5' overhang strand exactly one nucleotide 3' of the branch point. Structure- and sequence-specific nuclease that resolves holliday junctions (HJs) by symmetrically oriented incisions in two opposing strands near the junction point, thus leading to ligatable products; HJs are physical links between homologous DNA molecules that arise as central intermediary structures during homologous recombination and repair in meiotic and somatic cells. Structure-specific nuclease with 5'-flap endonuclease activity, preferentially cleaving static flaps 5' overhang strand exactly one nucleotide in the 3' direction of the branch point and, to lower extent, on the two neighboring positions. Also able to cleave double-stranded flap strand 1 one nucleotide in the 3' direction of the branch point. Together with MUS81, essential for the resolution of toxic replication structures to ensure genome stability, and to maintain telomere integrity and replication.
  • 基因功能參考文獻：
    1. SEND1 has at most a minor role in resolution of the Holliday junction but acts as an essential backup to MUS81 for resolution of toxic replication structures to ensure genome stability and to maintain telomere integrity. PMID: 26704385
    2. resolvase activity involves resolving holliday junctions by symmetrically oriented incisions in two opposing strands PMID: 25037209
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    XPG/RAD2 endonuclease family, GEN subfamily
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT3G48900

    STRING: 3702.AT3G48900.2

    UniGene: At.50684



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