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  4. Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor RAP2-12 (RAP2-12)

Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor RAP2-12 (RAP2-12)

  • 中文名稱：
    擬南芥RAP2-12重組蛋白
  • 貨號：
    CSB-YP882943DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥RAP2-12重組蛋白
  • 貨號：
    CSB-EP882943DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥RAP2-12重組蛋白
  • 貨號：
    CSB-EP882943DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥RAP2-12重組蛋白
  • 貨號：
    CSB-BP882943DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥RAP2-12重組蛋白
  • 貨號：
    CSB-MP882943DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RAP2-12
  • Uniprot No.：
  • 別名：
    RAP2-12; ERF074; At1g53910; T18A20.14; Ethylene-responsive transcription factor RAP2-12; Protein RELATED TO APETALA2 12
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-358
  • 氨基酸序列
    MCGGAIISDF IPPPRSRRVT SEFIWPDLKK NLKGSKKSSK NRSNFFDFDA EFEADFQGFK DDSSIDCDDD FDVGDVFADV KPFVFTSTPK PAVSAAAEGS VFGKKVTGLD GDAEKSANRK RKNQYRGIRQ RPWGKWAAEI RDPREGARIW LGTFKTAEEA ARAYDAAARR IRGSKAKVNF PEENMKANSQ KRSVKANLQK PVAKPNPNPS PALVQNSNIS FENMCFMEEK HQVSNNNNNQ FGMTNSVDAG CNGYQYFSSD QGSNSFDCSE FGWSDQAPIT PDISSAVINN NNSALFFEEA NPAKKLKSMD FETPYNNTEW DASLDFLNED AVTTQDNGAN PMDLWSIDEI HSMIGGVF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcription factor involved in the activation of hypoxic gene expression and in ethylene response. Partially redundant with RAP2-2. Acts as a downstream regulator in the ethylene signaling pathway.
  • 基因功能參考文獻：
    1. ERF74 and ERF75 play a redundant role in the up-regulation of RbohD transcription and enhance the ROS burst during the early stages of the stress response. [ERF74] PMID: 28164334
    2. Hypoxic root bending is inhibited by the group VII ethylene response factor (ERFVII) RAP2.12, as rap2.12-1 seedlings show exaggerated primary root bending. PMID: 28698356
    3. Results show that oxygen-sensing, mediating transcription factor RAP2.12 degradation is indispensable to optimize metabolic performance, plant growth, and development under both normoxic and hypoxic conditions. PMID: 27372243
    4. In Arabidopsis, an effective low oxygen-sensing response requires RAP2.12 stabilization followed by HRA1 induction to modulate the extent of the anaerobic response by negative feedback regulation of RAP2.12. PMID: 25226037
    5. identification of a conserved amino-terminal amino acid sequence of RAP2.12 to be dedicated to an oxygen-dependent sequence of post-translational modifications, which ultimately lead to degradation of RAP2.12 under aerobic conditions PMID: 22020282

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  • 亞細胞定位：
    Cell membrane. Nucleus. Note=Localizes to the plasma membrane under aerobic conditions, but accumulates in the nucleus upon hypoxia.
  • 蛋白家族：
    AP2/ERF transcription factor family, ERF subfamily
  • 組織特異性：
    Highly expressed in seedlings, leaves, flowers, siliques and germinating seeds.
  • 數據庫鏈接：

    KEGG: ath:AT1G53910

    STRING: 3702.AT1G53910.1

    UniGene: At.20952



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