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  4. Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase ORTHRUS 1 (ORTH1), partial

Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase ORTHRUS 1 (ORTH1), partial

  • 中文名稱：
    擬南芥ORTH1重組蛋白
  • 貨號：
    CSB-YP887784DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥ORTH1重組蛋白
  • 貨號：
    CSB-EP887784DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥ORTH1重組蛋白
  • 貨號：
    CSB-EP887784DOA-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥ORTH1重組蛋白
  • 貨號：
    CSB-BP887784DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥ORTH1重組蛋白
  • 貨號：
    CSB-MP887784DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ORTH1
  • Uniprot No.：
  • 別名：
    ORTH1; VIM3; At5g39550; MIJ24.3; E3 ubiquitin-protein ligase ORTHRUS 1; EC 2.3.2.27; Protein VARIANT IN METHYLATION 3; RING-type E3 ubiquitin transferase ORTHRUS 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    E3 ubiquitin-protein ligase. Participates in CpG methylation-dependent transcriptional regulation and epigenetic transcriptional silencing. Mediates ubiquitination with the E2 ubiquitin-conjugating enzymes UBC11, UBC8 and UBC8 homologs (e.g. UBC10, UBC11, UBC28 and UBC29) but not with UBC27, UBC30, UBC32, UBC34 and UBC36. Promotes methylation-mediated gene silencing leading, for example, to early flowering. Can bind to CpG, CpNpG, and CpNpN DNA motifs, with a strong preference for methylated forms, and with highest affinity for CpG substrate.
  • 基因功能參考文獻：
    1. VIM proteins function in transcriptional regulation via their roles in the MET1 DNA methylation pathway. PMID: 24762702
    2. ORTH proteins are ubiquitin E3 ligases that regulate DNA methylation. The ORTH1 RING domain promotes ubiquitylation, and overexpression leads to an altered flowering time because of decreased methylation of FWA. PMID: 18643997
    3. VIM1, VIM2, and VIM3 have overlapping functions in maintenance of global CpG methylation and epigenetic transcriptional silencing. PMID: 18704160
  • 亞細(xì)胞定位：
    Nucleus. Note=Broadly distributed in the nucleus and enriched in the heterochromatic chromocenters.
  • 組織特異性：
    Expressed in inflorescences and leaves.
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT5G39550

    STRING: 3702.AT5G39550.1

    UniGene: At.30336



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