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  4. Recombinant Arabidopsis thaliana Disease resistance protein RPS5 (RPS5), partial

Recombinant Arabidopsis thaliana Disease resistance protein RPS5 (RPS5), partial

  • 中文名稱：
    擬南芥RPS5重組蛋白
  • 貨號：
    CSB-YP526499DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥RPS5重組蛋白
  • 貨號：
    CSB-EP526499DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥RPS5重組蛋白
  • 貨號：
    CSB-EP526499DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥RPS5重組蛋白
  • 貨號：
    CSB-BP526499DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥RPS5重組蛋白
  • 貨號：
    CSB-MP526499DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RPS5
  • Uniprot No.：
  • 別名：
    RPS5; At1g12220; T28K15.5; Disease resistance protein RPS5; Resistance to Pseudomonas syringae protein 5; pNd3/pNd10
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Disease resistance (R) protein that specifically recognizes the avrPphB type III effector avirulence protein from Pseudomonas syringae. Also confers resistance against Hyaloperonospora parasitica (downy mildew). Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. Requires PBS1 to trigger the defense reaction against avrPphB. In case of infection by Pseudomonas syringae, AvrPphB triggers RPS5-mediated defense mechanism via the cleavage of PBS1, suggesting that the cleavage of PBS1 could trigger an exchange of ADP for ATP, thereby activating RPS5. May function as a fine-tuned sensor of alterations in the structure of the effector target PBS1.
  • 基因功能參考文獻：
    1. involved in photosynthesis, plant development, and cold stress tolerance PMID: 27006483
    2. RPS5 distinguishes PBS1 from closely related kinases based on a specific loop in the C-terminal half of PBS1. PMID: 24225654
    3. the RPS5 polymorphism may not be maintained through a tightly coupled interaction involving a single coevolved R gene and effector pair; more likely, the stable polymorphism is maintained through complex and diffuse community-wide interactions PMID: 25043057
    4. The RPS5 leucine-rich repeat domain functions to suppress RPS5 activation in the absence of PBS1 cleavage and promotes RPS5 activation in its presence. PMID: 22331412
  • 亞細胞定位：
    Cell membrane; Lipid-anchor.
  • 蛋白家族：
    Disease resistance NB-LRR family
  • 數據庫鏈接：

    KEGG: ath:AT1G12220

    STRING: 3702.AT1G12220.1

    UniGene: At.42084



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