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  4. Recombinant Arabidopsis thaliana DNA repair protein RAD51 homolog 1 (RAD51)

Recombinant Arabidopsis thaliana DNA repair protein RAD51 homolog 1 (RAD51)

  • 中文名稱：
    擬南芥RAD51重組蛋白
  • 貨號：
    CSB-YP019263DOA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥RAD51重組蛋白
  • 貨號：
    CSB-EP019263DOA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥RAD51重組蛋白
  • 貨號：
    CSB-EP019263DOA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥RAD51重組蛋白
  • 貨號：
    CSB-BP019263DOA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥RAD51重組蛋白
  • 貨號：
    CSB-MP019263DOA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RAD51
  • Uniprot No.：
  • 別名：
    RAD51; At5g20850; F22D1.20; DNA repair protein RAD51 homolog 1; Rad51-like protein 1; AtRAD51
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-342
  • 氨基酸序列
    MTTMEQRRNQ NAVQQQDDEE TQHGPFPVEQ LQAAGIASVD VKKLRDAGLC TVEGVAYTPR KDLLQIKGIS DAKVDKIVEA ASKLVPLGFT SASQLHAQRQ EIIQITSGSR ELDKVLEGGI ETGSITELYG EFRSGKTQLC HTLCVTCQLP MDQGGGEGKA MYIDAEGTFR PQRLLQIADR FGLNGADVLE NVAYARAYNT DHQSRLLLEA ASMMIETRFA LLIVDSATAL YRTDFSGRGE LSARQMHLAK FLRSLQKLAD EFGVAVVITN QVVAQVDGSA LFAGPQFKPI GGNIMAHATT TRLALRKGRA EERICKVISS PCLPEAEARF QISTEGVTDC KD
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Unwinds duplex DNA. Component of the meiotic recombination pathway. Seems to play a role in mediating chromosome homology search, chromosome pairing and synapsis at early stages and probably chromosome crossing-over at later stages in meiosis. Probably is involved in the repair of meiotic double strand breaks (DBSs) generated by AtSPO11-1 and in homologous recombination. Its function is dispensable for vegetative growth and root mitosis.
  • 基因功能參考文獻：
    1. Although the presence of RAD51 protein provides essential support for the action of DMC1, these results show no significant effect of the absence of RAD51 strand-exchange activity on meiotic crossing-over rates or patterns in different chromosomal regions or across the whole genome of Arabidopsis, strongly supporting the argument that DMC1 catalyses repair of all meiotic DNA breaks, not only non-sister cross-overs. PMID: 28797117
    2. RAD51 forms a protein complex with AtRAD51C-AtXRCC3 to facilitate RAD51 localization on chromosomes for meiotic recombination. PMID: 28562599
    3. The present study demonstrates for the first time the involvement of a host RAD51 protein in mungbean yellow mosaic India virus replication. PMID: 23575883
    4. Establishment and stabilisation of pairing of homologous centromeric and pericentromeric regions depends principally upon DMC1, while pairing and synapsis of euchromatic chromosome arms of homologues requires the presence of RAD51 PMID: 22532804
    5. AtRAD51 is required to ensure the fidelity of homologous recombination during interchromosomal exchanges. It may also be required to ensure the fidelity of homologous recombination in the interchromosomal exchanges initiated by AtDMC1. PMID: 22066484
    6. results show that loss of RAD51 caused hypersensitivity to the double-strand break-inducing agent bleomycin in P. patens but not Arabidopsis; data show importance of RAD51 for viability is independent of taxonomic position or complexity of an organism PMID: 17921313

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    RecA family, RAD51 subfamily
  • 組織特異性：
    Detected in various tissues. Higher expression in reproductive tissues than in vegetative tissues, with the highest expression level in young flower buds. At cellular level, is expressed at low levels in flower primordia, then at higher levels in young an
  • 數據庫鏈接：

    KEGG: ath:AT5G20850

    STRING: 3702.AT5G20850.1

    UniGene: At.110



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