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  4. Recombinant Arabidopsis thaliana DNA-directed RNA polymerase D subunit 1 (NRPD1), partial

Recombinant Arabidopsis thaliana DNA-directed RNA polymerase D subunit 1 (NRPD1), partial

  • 中文名稱：
    擬南芥NRPD1重組蛋白
  • 貨號：
    CSB-YP888773DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥NRPD1重組蛋白
  • 貨號：
    CSB-EP888773DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥NRPD1重組蛋白
  • 貨號：
    CSB-EP888773DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥NRPD1重組蛋白
  • 貨號：
    CSB-BP888773DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥NRPD1重組蛋白
  • 貨號：
    CSB-MP888773DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NRPD1
  • Uniprot No.：
  • 別名：
    NRPD1; NRPD1a; RMD3; RPD1; SDE4; SMD2; At1g63020; F16M19.19; F16P17.19; DNA-directed RNA polymerase IV subunit 1; DNA-directed RNA polymerase D subunit 1; AtNRPD1a; Nuclear RNA polymerase D 1a; EC 2.7.7.6; Protein RNA-DIRECTED DNA METHYLATION DEFECTIVE 3; Protein SILENCING DEFECTIVE 4; Protein SILENCING MOVEMENT DEFICIENT 2; RNA polymerase IV subunit 1a; POL IV 1a
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase IV which mediates 24-nt short-interfering RNAs (siRNA) accumulation. Implicated in siRNA-directed heterochromatin formation through the action of DCL3 and AGO4, and subsequent DNA methylation-dependent silencing of targeted sequences. Essential component of a self-reinforcing loop coupling de novo DNA methylation to siRNA production. Required for intercellular but not intracellular RNA interference (RNAi) leading to systemic post-transcriptional gene silencing. Involved in the maintenance of post-transcriptional RNA silencing.
  • 基因功能參考文獻：
    1. Pol IV-dependent (p4-)siRNAs are initially expressed in the maternal gametophyte and uniparentally expressed from maternal chromosomes in developing endosperm. PMID: 20074090
    2. results show that RNA polymerase IV (Pol IV, subunits NRPD1a and NRPD2) silences certain transposons and repetitive DNA in a short interfering RNA pathway involving RNA-dependent RNA polymerase 2 and Dicer-like 3(DCL3) [NRPD1a] PMID: 15692015
    3. RNA Pol IV helps produce siRNAs that target de novo cytosine methylation events required for facultative heterochromatin formation and higher-order heterochromatin associations.[NRPD1a; RNA Pol IV] PMID: 15766525
    4. RNA polymerase IV has a role in plant small RNA metabolism [RNAP IV] PMID: 17360559
    5. NRPD1a is required for reception of long-distance mRNA silencing. PMID: 17785412
    6. Mutants defective for the NRPD1a and NRPD1b alternative largest subunits of polymerase IV were tested for their ability to undergo RNA silencing and mantenance of transcription and post-transcriptional silencing PMID: 18433438

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  • 亞細胞定位：
    Nucleus. Note=Follows a punctate localization pattern.
  • 蛋白家族：
    RNA polymerase beta' chain family
  • 組織特異性：
    Mostly expressed in flowers, and, to a lower extent, in leaves.
  • 數據庫鏈接：

    KEGG: ath:AT1G63020

    STRING: 3702.AT1G63020.1

    UniGene: At.36192



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