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  4. Recombinant Arabidopsis thaliana DELLA protein GAI (GAI), partial

Recombinant Arabidopsis thaliana DELLA protein GAI (GAI), partial

  • 中文名稱：
    擬南芥GAI重組蛋白
  • 貨號：
    CSB-YP888780DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥GAI重組蛋白
  • 貨號：
    CSB-EP888780DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥GAI重組蛋白
  • 貨號：
    CSB-EP888780DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥GAI重組蛋白
  • 貨號：
    CSB-BP888780DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥GAI重組蛋白
  • 貨號：
    CSB-MP888780DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    GAI
  • Uniprot No.：
  • 別名：
    GAI; RGA2; At1g14920; F10B6.34; DELLA protein GAI; GRAS family protein 3; AtGRAS-3; Gibberellic acid-insensitive mutant protein; Restoration of growth on ammonia protein 2
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Transcription coactivator of the zinc finger transcription factors GAF1/IDD2 and ENY/IDD1 in regulation of gibberellin homeostasis and signaling. No effect of the BOI proteins on its stability. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes. Positively regulates XERICO expression. In contrast to RGA, it is less sensitive to GA. Its activity is probably regulated by other phytohormones such as auxin and ethylene. Involved in the regulation of seed dormancy and germination, including glucose-induced delay of seed germination.
  • 基因功能參考文獻：
    1. GAI negatively regulate dark-induced senescence and chlorophyll degradation. PMID: 29574486
    2. The DELLA is mono-O-fucosylated by the novel O-fucosyltransferase SPINDLY (SPY) in Arabidopsis thaliana. O-fucosylation activates DELLA by promoting its interaction with key regulators in brassinosteroid- and light-signaling pathways. PMID: 28244988
    3. this study provides direct link between melatonin and floral transition, and indicates the novel involvement of DELLAs-activated FLC in melatonin-mediated flowering in Arabidopsis. PMID: 26887824
    4. BBX24 physically interacts with DELLA proteins and alleviates DELLA-mediated repression of PIF4 activity. PMID: 25656233
    5. our data suggest that the miR171-SCL module is critical for mediating GA-DELLA signaling in the coordinate regulation of chlorophyll biosynthesis and leaf growth in light. PMID: 25101599
    6. Transcriptional analysis supported these results and revealed that a complex signaling network has responded to MgT in Arabidopsis. DELLA enhancement, which depends on ABI1, contributed to the remodeling growth and development of young seedlings PMID: 25074907
    7. determined the TF interactome of the Arabidopsis (Arabidopsis thaliana) DELLA protein GIBBERELLIN INSENSITIVE and screened a collection of conditional TF overexpressors in search of those that alter GA sensitivity PMID: 25118255
    8. RGA and GAI play distinct roles in seed germination, and GAI interacts with DAG1 in embryo development. PMID: 25064446
    9. showed that the WD-repeat/bHLH/MYB complex acts as a direct target of DELLAs in the GA pathway and that both DELLAs and JAZs interacted with the WD-repeat/bHLH/MYB complex to mediate synergism between GA and JA signaling PMID: 24659329
    10. The removal of DELLA repression highly upregulated certain genes on various hormone pathways, suggesting that gibberellin signaling acts upstream of the jasmonic acid, salicylic acid, and ethylene pathways in regulating leaf senescence. PMID: 24576761
    11. Further analyses demonstrated that TOPP4 is able to directly bind and dephosphorylate RGA and GAI, confirming that the TOPP4-controlled phosphorylation status of DELLAs is associated with their stability PMID: 25010794
    12. DELLA protein functions as a transcriptional activator through the DNA binding of the indeterminate domain family proteins. PMID: 24821766
    13. Atgai mRNA can move from companion cells to sieve tubes and the translated product remains at the sites to which it is transported. PMID: 24144190
    14. GAI requires BOTRYTIS SUSCEPTIBLE1 INTERACTORs (BOIs) to inhibit GA responses such as seed germination, phase transition, and flowering. PMID: 23482857
    15. Results show that the induction of GAI activity has an almost immediate effect on gene expression. PMID: 21904598
    16. Through transgenic modification, DELLA-less versions of GAI (gai) and RGL1 (rgl1) in a Populus tree have profound, dominant effects on phenotype, producing pleiotropic changes in morphology and metabolic profiles. PMID: 16404575
    17. PIL5 binds directly to GAIpromoters but not to Giberellic and Abscisic metabolic gene promoters in arabidopsis. PMID: 17449805
    18. Data show that gai gene apple transgenic clones showed reduced rooting ability, especially for the extremely compact ones. PMID: 17932677
    19. A study evaluting ethylene-giberillic acid crosstalk in gibberellin-insensitive (gai), ethylene-overproducing 2-1 (eto2-1) double mutant A. thaliana is reported. PMID: 18078472
    20. The RNA mobile elements in GAI RNA play important roles in RNA movement. PMID: 19453448

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    GRAS family, DELLA subfamily
  • 組織特異性：
    Ubiquitously expressed. Expressed in rosette leaves, roots, stems and inflorescences of greenhouse grown.
  • 數據庫鏈接：

    KEGG: ath:AT1G14920

    STRING: 3702.AT1G14920.1

    UniGene: At.15032



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