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  4. Recombinant Arabidopsis thaliana Cyclin-dependent kinase A-1 (CDKA-1)

Recombinant Arabidopsis thaliana Cyclin-dependent kinase A-1 (CDKA-1)

  • 中文名稱：
    Recombinant Arabidopsis thaliana Cyclin-dependent kinase A-1(CDKA-1)
  • 貨號：
    CSB-YP326117DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Cyclin-dependent kinase A-1(CDKA-1)
  • 貨號：
    CSB-EP326117DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Cyclin-dependent kinase A-1(CDKA-1)
  • 貨號：
    CSB-EP326117DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Cyclin-dependent kinase A-1(CDKA-1)
  • 貨號：
    CSB-BP326117DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Cyclin-dependent kinase A-1(CDKA-1)
  • 貨號：
    CSB-MP326117DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    CDKA-1
  • Uniprot No.：
  • 別名：
    CDKA-1; CDC2; CDC2A; At3g48750; T21J18.20Cyclin-dependent kinase A-1; CDKA;1; EC 2.7.11.22; EC 2.7.11.23; Cell division control protein 2 homolog A; CDC2aAt
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-294
  • 氨基酸序列
    MDQYEKVEKI GEGTYGVVYK ARDKVTNETI ALKKIRLEQE DEGVPSTAIR EISLLKEMQH SNIVKLQDVV HSEKRLYLVF EYLDLDLKKH MDSTPDFSKD LHMIKTYLYQ ILRGIAYCHS HRVLHRDLKP QNLLIDRRTN SLKLADFGLA RAFGIPVRTF THEVVTLWYR APEILLGSHH YSTPVDIWSV GCIFAEMISQ KPLFPGDSEI DQLFKIFRIM GTPYEDTWRG VTSLPDYKSA FPKWKPTDLE TFVPNLDPDG VDLLSKMLLM DPTKRINARA ALEHEYFKDL GGMP
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Involved in the control of the cell cycle. Essential for both G1/S and G2/M (mitosis) phase transitions. Functions in cell morphogenesis as well as cell proliferation. Required for cell division (entry into mitosis) of the generative cell in male gametogenesis. Required to trigger guard mother cells (GMC) symmetric divisions at the late stage of stomatal development, probably via the regulation of G1 to S transition in the cell cycle. Required for the function of SPCH in entering the stomatal lineage. Promotes divisions in the guard cells (GCs) after the guard mother cells (GMC) symmetric division when in the presence of CYCD3-2 via the phosphorylation of SPCH.
  • 基因功能參考文獻(xiàn)：
    1. CDKA;1 and CYCD3;2 are required for the terminal division in the stomatal lineage. PMID: 24687979
    2. Data indicate that the in vivo confirmation of substrates of CDKA;1 showing a direct link between cell proliferation and the control of the redox state. PMID: 22098373
    3. The crucial function of CDKA;1 is the control of the plant Retinoblastoma homolog RBR1 and codepletion of RBR1 and CDKA;1 rescued most defects of cdka;1 mutants. PMID: 22595674
    4. Expression of a dominant negative CDKA;1 allele under the control of the STM promoter perturbs post-embryonic development. Inhibition of CDK activity at the shoot apex results in premature differentiation of shoot apical meristem cells. PMID: 20659279
    5. When a single cdka;1 sperm was delivered, either female gamete could be fertilized leading to similar proportions of seeds containing either a single endosperm or a single embryo. PMID: 20610483
    6. However, we show here that the DNA damage checkpoint in Arabidopsis can also operate independently of the phosphorylation of CDKA;1. PMID: 19948791
    7. CDC2A participates in the fertilization process of endosperm PMID: 16311592
    8. The balance between cell division and differentiation is regulated through the interaction between CDKA;1 and the antiphosphatase PAS2. [CDKA;1] PMID: 16698944
    9. The Arabidopsis thaliana Cdc2(+)/Cdc28 homolog CDKA;1 is also phosphorylated in the T-loop and that phosphorylation at the conserved Thr-161 residue is essential for its function. PMID: 17369369
    10. phosphorylation of T161 in Arabidopsis CDKA;1 is essential for cell division during male gametogenesis PMID: 17764501

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus. Note=Mainly cytoplasmic. Nuclear distribution increases after binding to ICK1/KRP1.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
  • 組織特異性：
    Expressed in roots, stems, flowers and siliques.
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT3G48750

    STRING: 3702.AT3G48750.1

    UniGene: At.24166



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