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  4. Recombinant Arabidopsis thaliana Cellulose synthase A catalytic subunit 1 [UDP-forming] (CESA1), partial

Recombinant Arabidopsis thaliana Cellulose synthase A catalytic subunit 1 [UDP-forming] (CESA1), partial

  • 中文名稱：
    Recombinant Arabidopsis thaliana Cellulose synthase A catalytic subunit 1 [UDP-forming](CESA1) ,partial
  • 貨號：
    CSB-YP525056DOA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Cellulose synthase A catalytic subunit 1 [UDP-forming](CESA1) ,partial
  • 貨號：
    CSB-EP525056DOA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Cellulose synthase A catalytic subunit 1 [UDP-forming](CESA1) ,partial
  • 貨號：
    CSB-BP525056DOA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Cellulose synthase A catalytic subunit 1 [UDP-forming](CESA1) ,partial
  • 貨號：
    CSB-MP525056DOA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    CESA1
  • Uniprot No.：
  • 別名：
    CESA1; RSW1; At4g32410; F8B4.110Cellulose synthase A catalytic subunit 1 [UDP-forming]; AtCesA1; EC 2.4.1.12; Protein RADIALLY SWOLLEN 1; AtRSW1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation. Involved in the primary cell wall formation. Required during embryogenesis for cell elongation, orientation of cell expansion and complex cell wall formations, such as interdigitated pattern of epidermal pavement cells, stomatal guard cells and trichomes. Plays a role in lateral roots formation, but seems not necessary for the development of tip-growing cells such as root hairs. The presence of each protein CESA1 and CESA6 is critical for cell expansion after germination.
  • 基因功能參考文獻：
    1. AtCesA6-null mutants showed a reduced cell elongation of young seedlings with little impact on cell division, which consequently affected cell wall integrity and biomass yield of mature plants. In comparison, rsw1 seedlings exhibited a strong defect in both cell elongation and division at restrictive temperature, whereas the IRX3 mutant showed normal seedling growth. PMID: 29514326
    2. Our data support a model in which distinct spatiotemporal features of active CSCs (cellulose synthase (CESA) complex))during PCW and SCW synthesis contribute to the formation of cellulose with distinct structure and organization in PCWs and SCWs of Arabidopsis thaliana PMID: 27647923
    3. microscopy showed that two altered versions of Atcesa1 could rescue cell wall phenotypes observed in the mutant background line. Overall, the data show that the FxVTxK motif is functionally important in two phylogenetically distant plant CESAs. PMID: 26646446
    4. A low resolution structure of the catalytic domain of CESA1 determined by small-angle scattering techniques provides the first experimental evidence for the self-assembly of CESA1 into a stable trimer. PMID: 26556795
    5. The stoichiometry of the cellulose synthase catalytic subunits (CESAs) in the primary cell wall CSC was determined and CESA1, CESA3, and CESA6 are present in a 1:1:1 molecular ratio. PMID: 25352273
    6. computational findings on transmembrane helices (TMHs) 5 and 6 in three CESAs of Arabidopsis thaliana (AtCESA1, 3, and 6), the Atcesa3(ixr1-2) mutant, and a novel missense mutation in AtCESA1, are reported. PMID: 25262226
    7. Relatively high sequence conservation between plant CESAs allowed mapping of known mutations and two previously undescribed mutations that perturb cellulose synthesis in Arabidopsis thaliana to their analogous positions in the modeled structure. PMID: 23592721
    8. CESA1(A903V) and CESA3(T942I) have modified microfibril structure in terms of crystallinity and suggest that in plants, as in bacteria, crystallization biophysically limits polymerization. PMID: 22375033
    9. Data suggest that phosphorylation of CESA1 affects polar interactions with microtubules that may regulate the length or quantity of a subset of cellulose microfibrils and that this, in turn, alters microfibril structure in the primary cell wall. PMID: 20855602
    10. The effects of CESA1 and CESA3 mutations and chimeric proteins on the temperature sensitivity of A. thaliana is reported. PMID: 16891551
    11. CESA 1, 3, and 6 form a complex which is important for cellulose synthesis. PMID: 18495638

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Glycosyltransferase 2 family, Plant cellulose synthase subfamily
  • 組織特異性：
    Expressed in germinating seeds, seedlings, roots, stems, shoots leaves and flowers, but not in mature flowers.
  • 數據庫鏈接：

    KEGG: ath:AT4G32410

    STRING: 3702.AT4G32410.1

    UniGene: At.21246



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