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  4. Recombinant Arabidopsis thaliana B3 domain-containing transcription factor FUS3 (FUS3)

Recombinant Arabidopsis thaliana B3 domain-containing transcription factor FUS3 (FUS3)

  • 中文名稱：
    擬南芥FUS3重組蛋白
  • 貨號：
    CSB-YP888843DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥FUS3重組蛋白
  • 貨號：
    CSB-EP888843DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥FUS3重組蛋白
  • 貨號：
    CSB-EP888843DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥FUS3重組蛋白
  • 貨號：
    CSB-BP888843DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥FUS3重組蛋白
  • 貨號：
    CSB-MP888843DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    FUS3
  • Uniprot No.：
  • 別名：
    FUS3; At3g26790; MDJ14.4B3 domain-containing transcription factor FUS3; Protein FUSCA3
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-313
  • 氨基酸序列
    MMVDENVETK ASTLVASVDH GFGSGSGHDH HGLSASVPLL GVNWKKRRMP RQRRSSSSFN LLSFPPPMPP ISHVPTPLPA RKIDPRKLRF LFQKELKNSD VSSLRRMILP KKAAEAHLPA LECKEGIPIR MEDLDGFHVW TFKYRYWPNN NSRMYVLENT GDFVNAHGLQ LGDFIMVYQD LYSNNYVIQA RKASEEEEVD VINLEEDDVY TNLTRIENTV VNDLLLQDFN HHNNNNNNNS NSNSNKCSYY YPVIDDVTTN TESFVYDTTA LTSNDTPLDF LGGHTTTTNN YYSKFGTFDG LGSVENISLD DFY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcription regulator involved in gene regulation during late embryogenesis. Its expression to the epidermis is sufficient to control foliar organ identity by regulating positively the synthesis abscisic acid (ABA) and negatively gibberellin production. Negatively regulates TTG1 in the embryo. Positively regulates the abundance of the ABI3 protein in the seed. Cooperates with KIN10 to regulate developmental phase transitions and lateral organ development and act both as positive regulators of abscisic acid (ABA) signaling during germination.
  • 基因功能參考文獻：
    1. AIP2 targets FUS3 for degradation and plays a role in cotyledon development and flowering time in Arabidopsis. PMID: 28369580
    2. FUS3 phosphorylation and SnRK1 are required for embryogenesis and integration of environmental cues to ensure the survival of the progeny. PMID: 28922765
    3. LEAFY COTYLEDON2 (LEC2) was identified as an interacting factor of FUS3, and demonstrated that these two homologous B3 transcription factors interact to bind to the auxin biosynthesis gene YUCCA4 (YUC4) and synergistically activate its transcription during lateral roots formation. PMID: 27878992
    4. TT2 is directly bound to the regulatory region of FUSCA3, and mediates the expression of numerous genes in the fatty acid biosynthesis pathway. PMID: 24397827
    5. Examination of direct targets of FUS3 reveals that FUS3 acts primarily or exclusively as a transcriptional activator. PMID: 23314941
    6. Activation of FUS3 after germination dampens the expression of genes involved in the biosynthesis and response to the plant hormone ethylene, whereas a loss-of-function fus3 mutant shows many phenotypes consistent with increased ethylene signaling. PMID: 22348746
    7. FUS3 contributes to the delay of seed germination at high temperature. PMID: 22279962
    8. The genes positively controlled by FUS3 are not confined to previously known seed maturation-related genes and include those involved in the production of secondary metabolites and those involved in primary metabolism. PMID: 21045071
    9. The C-terminal domain is required for normal FUS3 function and sensitivity to abscisic acid and gibberellic acid, and negatively regulates mRNA and protein levels. PMID: 20663088
    10. In this study, it was shown that gibberellin (GA) hormone biosynthesis is regulated by LEC2 and FUS3 pathways. PMID: 15516508
    11. At2S3 activation by FUS3 was rapid but CRC induction by FUS3 with abscissic acid[ABA] and by ABA followed by presence of FUS3 took longer, suggesting involvement of an indirect mechanism requiring the synthesis of intermediate regulatory factor(s) PMID: 15695463
    12. Total repression of embryogenic potential was observed in double (lec1 lec2, lec1 fus3, lec2 fus3) and triple (fus3 lec1 lec2) mutants. PMID: 16034595
    13. Results show that Significant differences in histone modifications at the phas promoter were mediated by FUS3 and PvALF, suggesting that they function through different epigenetic mechanisms. PMID: 18038114
    14. FUS3 function is restricted to the acquisition of embryo-dependent seed dormancy, the determination of cotyledonary cell identity, and the synthesis and accumulation of storage compounds. PMID: 18343361

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  • 亞細胞定位：
    Nucleus.
  • 組織特異性：
    Expressed in cotyledons and hypocotyls.
  • 數據庫鏈接：

    KEGG: ath:AT3G26790

    STRING: 3702.AT3G26790.1

    UniGene: At.6029



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