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  4. Recombinant Arabidopsis thaliana Auxin efflux carrier component 3 (PIN3), partial

Recombinant Arabidopsis thaliana Auxin efflux carrier component 3 (PIN3), partial

  • 中文名稱：
    擬南芥PIN3重組蛋白
  • 貨號：
    CSB-YP890278DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥PIN3重組蛋白
  • 貨號：
    CSB-EP890278DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥PIN3重組蛋白
  • 貨號：
    CSB-EP890278DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥PIN3重組蛋白
  • 貨號：
    CSB-BP890278DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥PIN3重組蛋白
  • 貨號：
    CSB-MP890278DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PIN3
  • Uniprot No.：
  • 別名：
    PIN3; At1g70940; F15H11.14; Auxin efflux carrier component 3; AtPIN3
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Acts as a component of the auxin efflux carrier. Seems to be involved in the lateral auxin transport system and mediates tropic growth. Coordinated polar localization of PIN3 is directly regulated by the vesicle trafficking process.
  • 基因功能參考文獻：
    1. Two mechanistically distinct PIN3 polarization events redirect auxin fluxes at different time points of the gravity response: first, gravity-mediated redirection of PIN3-mediated auxin flow toward the lower hypocotyl side, where auxin gradually accumulates and promotes growth, and later PIN3 polarization to the opposite cell side, depleting this auxin maximum to end the bending. PMID: 27773568
    2. Functional interplay between protein kinase CK2 and salicylic acid sustains PIN3 transcriptional expression. PMID: 24547808
    3. asymmetric distribution of auxin is essential for root negative phototropism, and is regulated by blue-light-induced PIN3 polarization PMID: 23888933
    4. Sequential induction of auxin efflux (PIN3) and influx (LAX3) carriers regulates lateral root emergence in Arabidopsis. PMID: 24150423
    5. Arabidopsis thaliana PIN3 auxin transporter is required for the asymmetric auxin distribution for the gravitropic response. PMID: 21569134
    6. The phosphorylation code of PIN3-hydrophilic loop is operational for the determination of PIN3 polarity in the Arabidopsis guard cell. PMID: 22902703
    7. The loss of PIN3 causes a dramatic delay in the onset of Lateral root initiation (LRI) after founder cell (FC) are specified, which can be fully recovered by the expression of PIN3 exclusively in the endodermis. PMID: 23178590
    8. PIN3-mediated auxin gradients participate in pulse-induced phototropism. PMID: 22843667
    9. Mutation of phosphorylation sites caused defects in PIN3-mediated biological processes such as auxin efflux activity, auxin maxima formation, root growth, and root gravitropism. PMID: 22519832
    10. Data show that light polarizes the cellular localization of the auxin efflux carrier PIN3 in hypocotyl endodermis cells, resulting in changes in auxin distribution and differential growth. PMID: 21394084
    11. Data show that pin3 mutants are outcompeted by wild-type neighbors who suppress fitness of pin3-3 by 40%. PMID: 21149713
    12. Cold stress blocks the early phase of gravity response by inhibiting the lateral relocalization of PIN3. PMID: 20040541
    13. NOV is required for cell type-specific expression of PIN3 in the root. PMID: 19880797
    14. findings show that both ARL2 and ARG1 function in a gravity signal transduction pathway with PIN3, an auxin efflux facilitator that is expressed in the statocytes PMID: 18047472

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Auxin efflux carrier (TC 2.A.69.1) family
  • 組織特異性：
    Predominantly expressed at the lateral side of shoot endodermis cells as well as root pericycle and columella cells.
  • 數據庫鏈接：

    KEGG: ath:AT1G70940

    STRING: 3702.AT1G70940.1

    UniGene: At.11573



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