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  4. Recombinant Arabidopsis thaliana 65-kDa microtubule-associated protein 3 (MAP65-3), partial

Recombinant Arabidopsis thaliana 65-kDa microtubule-associated protein 3 (MAP65-3), partial

  • 中文名稱：
    擬南芥MAP65-3重組蛋白
  • 貨號：
    CSB-YP884243DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥MAP65-3重組蛋白
  • 貨號：
    CSB-EP884243DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥MAP65-3重組蛋白
  • 貨號：
    CSB-EP884243DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥MAP65-3重組蛋白
  • 貨號：
    CSB-BP884243DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥MAP65-3重組蛋白
  • 貨號：
    CSB-MP884243DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MAP65-3
  • Uniprot No.：
  • 別名：
    MAP65-3; PLE; At5g51600; K17N15.1565-kDa microtubule-associated protein 3; AtMAP65-3; Protein PLEIADE
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Microtubule-associated protein that plays a critical role in organizing the mitotic microtubule array during both early and late mitosis in all plant organs. Essential for the cytokinesis, especially in roots, by maintaining the integrity of the overlapped microtubules in the phragmoplast. Required during root morphogenesis. Needed for giant cell development during root knot nematode infection, where cytokinesis is initiated but not completed.
  • 基因功能參考文獻：
    1. Cell cycle-regulated PLEIADE/AtMAP65-3 links membrane and microtubule dynamics during plant cytokinesis. PMID: 27420177
    2. downy mildew and powdery mildew stimulate AtMAP65-3 expression to down-regulate salicylic acid signaling for infection. PMID: 26798028
    3. MAP65-3 selectively cross-linked interdigitating microtubules (IMTs) to allow antiparallel microtubules (MTs) to be closely engaged in the phragmoplast. PMID: 21873565
    4. Data conclude that MAP65-3 contains a distinct C-terminal MT binding site with a specific role in cross-linking antiparallel MTs toward their plus ends in the phragmoplast. PMID: 22570443
  • 亞細胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, phragmoplast. Note=Locates only to the mitotic MT arrays. Present in MT cortical arrays just before mitosis. Associates to MT in preprophase band, during anaphase, and in phragmoplast, including midzone. Distributed diffusely through the cytoplasm during metaphase only. At the end of cytokinesis, present only at the cell periphery, forming a ring around the newly formed cell plate. During root knot nematode infection, targeted to the giant cell mini cell plate.
  • 蛋白家族：
    MAP65/ASE1 family
  • 組織特異性：
    Expressed in all tissues enriched in dividing cells, such as the root and shoot apical meristem, foliar primordia, and young leaves, and embryos.
  • 數據庫鏈接：

    KEGG: ath:AT5G51600

    STRING: 3702.AT5G51600.1

    UniGene: At.29670



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