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  4. Recombinant Arabidopsis thaliana 26S proteasome regulatory subunit 4 homolog A (RPT2A)

Recombinant Arabidopsis thaliana 26S proteasome regulatory subunit 4 homolog A (RPT2A)

  • 中文名稱：
    擬南芥RPT2A重組蛋白
  • 貨號：
    CSB-YP874568DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥RPT2A重組蛋白
  • 貨號：
    CSB-EP874568DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥RPT2A重組蛋白
  • 貨號：
    CSB-EP874568DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥RPT2A重組蛋白
  • 貨號：
    CSB-BP874568DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥RPT2A重組蛋白
  • 貨號：
    CSB-MP874568DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RPT2A
  • Uniprot No.：
  • 別名：
    RPT2A; HLR; At4g29040; F19B15.70; F25O24.6; 26S proteasome regulatory subunit 4 homolog A; 26S proteasome AAA-ATPase subunit RPT2a; 26S proteasome subunit 4 homolog A; Protein HALTED ROOT; Regulatory particle triple-A ATPase subunit 2a
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-443
  • 氨基酸序列
    MGQGPSGGLN RQGDRKPDGG DKKEKKFEPA APPARVGRKQ RKQKGPEAAA RLPTVTPSTK CKLRLLKLER IKDYLLMEEE FVANQERLKP QEEKAEEDRS KVDDLRGTPM SVGNLEELID ENHAIVSSSV GPEYYVGILS FVDKDQLEPG CSILMHNKVL SVVGILQDEV DPMVSVMKVE KAPLESYADI GGLEAQIQEI KEAVELPLTH PELYEDIGIK PPKGVILYGE PGTGKTLLAK AVANSTSATF LRVVGSELIQ KYLGDGPKLV RELFRVADDL SPSIVFIDEI DAVGTKRYDA HSGGEREIQR TMLELLNQLD GFDSRGDVKV ILATNRIESL DPALLRPGRI DRKIEFPLPD IKTRRRIFQI HTSKMTLSED VNLEEFVMTK DEFSGADIKA ICTEAGLLAL RERRMKVTHP DFKKAKEKVM FKKKEGVPEG LYM
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (Probable). Interacts with transit peptides of proteins targeted to the chloroplast, and may be involved in the degradation of unimported plastid protein precursors. Is required for the maintenance of postembryonic root and shoot meristems. Has a specific role in the regulation of organs size. Acts redundantly with RPT2B in the regulation of gametogenesis. With RPT2B plays a critical role in 26S proteasome assembly. Acts as an upstream signaling component for inducing both defense and morphological phenotypes in the constitutive active uni-1D mutant. Acts as negative regulator of endoreduplication in trichome cells. May function after the completion of the third endoreduplication step (8C to 16C) mediated by RHL1. Acts as negative regulator of transcriptional gene silencing (TGS) at specific endogenous genes through DNA methylation. Promotes post-transcriptional gene silencing (PTGS) by limiting the degradation of transgene aberrant RNAs by the RNA quality control (RQC) machinery, thus favoring their entry into cytoplasmic siRNA bodies where they can trigger PTGS. Involved in tolerance to zinc deficiency, possibly through alleviation of oxidative stresses or processing of poly-ubiquitinated proteins. Required for resistance to the fungal pathogen Golovinomyces cichoracearum.
  • 基因功能參考文獻：
    1. Both the transgene and endogenous RD29A promoter regions were hypermethylated at CG and non-CG contexts in the rpt2a mutant. PMID: 22615900
    2. Loss of RPT2a destabilizes the 26S proteasome. PMID: 22158466
    3. RPT2a and RPT2b contribute differently to the proteasome activity required for root apical meristem growth and gametophyte development. PMID: 21784786
    4. RPT2a and RPT2b, the subunits of the 26S proteasome AAA-ATPase, are UNI-interacting proteins. uni-1D-induced morphological phenotypes during early vegetative stage were suppressed by loss of function of RPT2a, but not RPT2b. PMID: 21791544
    5. Data suggest that RPT2a functions to negatively regulate endocycle progression following completion of the third endoreduplication step mediated by RHL1. PMID: 20195883
    6. RPT2a has a specific role in the regulation of leaf organ size that involves control of endoreduplication and cell size, rather than alteration of leaf cell number. PMID: 19500299

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  • 亞細胞定位：
    Cytoplasm, P-body. Nucleus.
  • 蛋白家族：
    AAA ATPase family
  • 組織特異性：
    Preferentially expressed in the root and shoot apical meristem.
  • 數據庫鏈接：

    KEGG: ath:AT4G29040

    STRING: 3702.AT4G29040.1

    UniGene: At.24596



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