Recombinant Arabidopsis thaliana 12-oxophytodienoate reductase 1 (OPR1)
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中文名稱:擬南芥OPR1重組蛋白
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貨號:CSB-EP817164DOA-B
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說明書:
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規格:
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來源:E.coli
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共軛:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他:
產品詳情
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純度:>85% (SDS-PAGE)
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基因名:OPR1
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Uniprot No.:
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別名:OPR1; At1g76680; F28O16.5; 12-oxophytodienoate reductase 1; EC 1.3.1.42; 12-oxophytodienoate-10,11-reductase 1; AtOPR1; OPDA-reductase 1; FS-AT-I
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種屬:Arabidopsis thaliana (Mouse-ear cress)
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蛋白長度:full length protein
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表達區域:1-372
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氨基酸序列MENGEAKQSV PLLTPYKMGR FNLSHRVVLA PLTRQRSYGN VPQPHAAIYY SQRTTPGGFL ITEATGVSDT AQGYQDTPGI WTKEHVEAWK PIVDAVHAKG GIFFCQIWHV GRVSNSGFQP NGKAPISCSD KPLMPQIRSN GIDEALFTPP RRLGIEEIPG IVNDFRLAAR NAMEAGFDGV EIHGANGYLI DQFMKDTVND RTDEYGGSLQ NRCKFPLEIV DAVAKEIGPD RVGIRLSPFA DYMESGDTNP GALGLYMAES LNKYGILYCH VIEARMKTMG EVHACPHTLM PMRKAFKGTF ISAGGFTRED GNEAVSKGRT DLVAYGRWFL ANPDLPKRFQ VDAPLNKYDR PTFYTSDPVV GYTDYPFLES TA
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蛋白標簽:Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式:Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet :Please contact us to get it.
靶點詳情
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功能:Specifically cleaves olefinic bonds in alpha,beta-unsaturated carbonyls and may be involved in detoxification or modification of these reactive compounds. May be involved in the biosynthesis or metabolism of oxylipin signaling molecules (Probable). In vitro, reduces 9R,13R-12-oxophytodienoic acid (9R,13R-OPDA) to 9R,13R-OPC-8:0, but only poorly 9S,13S-OPDA, the natural precursor of jasmonic acid. Can detoxify the explosive 2,4,6-trinitrotoluene (TNT) in vitro and in vivo by catalyzing its nitroreduction to form hydroxylamino-dinitrotoluene (HADNT).
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基因功能參考文獻:
- Crystal structure provides a basis for a more detailed evaluation of 12-oxophytodienoate reductase isoform 3, a key enzyme required for jasmonate biosynthesis PMID: 15468319
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亞細胞定位:Cytoplasm.
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蛋白家族:NADH:flavin oxidoreductase/NADH oxidase family
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組織特異性:Mostly expressed in roots, also present in leaves, shoots and flowers. More abundant in cotyledons. In more details, expressed in peduncles, sepals, petals, around the abscission zone of siliques, maturing siliques and developing seeds.
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數據庫鏈接:
KEGG: ath:AT1G76680
UniGene: At.11155
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