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  4. Recombinant Anthopleura elegantissima Toxin APETx1

Recombinant Anthopleura elegantissima Toxin APETx1

  • 中文名稱：
    Recombinant Anthopleura elegantissima Toxin APETx1
  • 貨號：
    CSB-YP351057DNA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Anthopleura elegantissima Toxin APETx1
  • 貨號：
    CSB-EP351057DNA
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Anthopleura elegantissima Toxin APETx1
  • 貨號：
    CSB-EP351057DNA-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Anthopleura elegantissima Toxin APETx1
  • 貨號：
    CSB-BP351057DNA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Anthopleura elegantissima Toxin APETx1
  • 貨號：
    CSB-MP351057DNA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 別名：
    ; Kappa-actitoxin-Ael2a; Kappa-AITX-Ael2a; Toxin APETx1
  • 種屬：
    Anthopleura elegantissima (Sea anemone)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-42
  • 氨基酸序列
    GTTCYCGKTI GIYWFGTKTC PSNRGYTGSC GYFLGICCYP VD
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

問答及客戶評論

■ 常見問題解答
Q:

Regarding your protein CSB-EP351057DNA, could you please provide some informations?
Additionally, I'm interested to know if this protein was “active” or not – do you have any expectation of this protein’s activity?

A:
Thanks for your inquiry.
Recombinant Anthopleura Toxin APETx1
CSB-EP351057DNA >> E.coli
Expression Region:1-42aa; Full length.
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 6xHis-SUMO-tagged.
Sequence:

GTTCYCGKTIGIYWFGTKTCPSNRGYTGSCGYFLGICCYPVD


Regarding the activity of this protein, sorry, we haven't validated the activity for the time being, so we can't 100% guarantee.
Here are two literatures for your reference:
http://molpharm.aspetjournals.org/content/molpharm/64/1/59.full.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3413854/
These literaures mainly study the function of this protein, the target protein is extracted or syntheszied from the other lab,
What we provide is the full length protein and it's purified under very mild condition, in theory, it has great potential to be active, which can be used for the related functional studies.

靶點詳情

  • 功能：
    Peptide with both antimicrobial and neurotoxin activities. This toxin acts both on ERG potassium channels and sodium channels. It potently and reversibly inhibits human Kv11.1/KCNH2/ERG1 (IC(50)=34 nM), rat Kv11.1/KCNH2/ERG1 and Kv11.3/KCNH7/ERG3 voltage-gated potassium channels in a similar potency. It acts as a gating-modifier toxin that shifts the voltage-dependence of ERG activation in the positive direction and suppresses its current amplitudes elicited by strong depolarizing pulses. On sodium channels, it blocks Nav1.2/SCN2A (EC(50)=31 nM), Nav1.3/SCN3A, Nav1.4/SCN4A, Nav1.5/SCN5A, Nav1.6/SCN8A, Nav1.8/SCN10A (EC(50)=92 nM). It may act by binding at site 1 or close by, only when the pore is in an open configuration. Shows antibacterial activity against the Gram-negative bacterium S.typhimurium, but not on the bacteria B.subtilis, S.aureus, and P.aeruginosa. In vivo, this toxin does not induce neurotoxic symptoms when injected into mice.
  • 亞細胞定位：
    Secreted. Nematocyst.
  • 蛋白家族：
    Sea anemone type 3 (BDS) potassium channel toxin family


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