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  4. Recombinant Adeno-associated virus 2 DNA replication protein (REP), partial

Recombinant Adeno-associated virus 2 DNA replication protein (REP), partial

  • 中文名稱：
    Recombinant Adeno-associated virus 2 DNA replication protein(REP) ,partial
  • 貨號：
    CSB-YP365856ADB
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Adeno-associated virus 2 DNA replication protein(REP) ,partial
  • 貨號：
    CSB-EP365856ADB
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Adeno-associated virus 2 DNA replication protein(REP) ,partial
  • 貨號：
    CSB-EP365856ADB-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Adeno-associated virus 2 DNA replication protein(REP) ,partial
  • 貨號：
    CSB-BP365856ADB
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Adeno-associated virus 2 DNA replication protein(REP) ,partial
  • 貨號：
    CSB-MP365856ADB
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Rep68
  • Uniprot No.：
  • 別名：
    Rep68; Protein Rep68; EC 3.6.4.12
  • 種屬：
    Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays an essential role in the initiation of viral DNA synthesis. Binds specifically to an inverted terminal repeat element (ITR) on the 3' and 5' ends of the viral DNA, where it cleaves a site specifically to generate a priming site for initiation of the synthesis of a complementary strand. Plays also a role as transcriptional regulator, DNA helicase and as key factor in site-specific integration of the viral genome. Inhibits the host cell cycle G1/S and G2/M transitions. These arrests may provide essential cellular factors for viral DNA replication.
  • 基因功能參考文獻：
    1. this study first determined the x-ray structure of AAV-2 Rep68 OBD in complex with the AAVS1 DNA site PMID: 26370092
    2. Inhibition of herpes simplex virus 1 replication involves direct binding of Rep68/Rep78 to the HSV-1 genome. PMID: 26292324
    3. This article presents the solution structure of Adeno-associated virus 2 Rep68 using small-angle X-ray scattering. PMID: 26314310
    4. Results indicate that transient expression of Rep68/78 in HeLa cells improved integration of the gene of interest at the AAVS1 locus in a site-specific manner. PMID: 23034483
    5. These results support the idea of a Rep68 molecule that exhibits the flexible oligomeric behavior needed to perform the wide range of functions occurring during the adeno-associated virus life cycle. PMID: 23152528
    6. we described a detailed structural comparison between the helicase domains of AAV-2 Rep proteins PMID: 22719256
    7. Rep interacts with cellular factors and herpes simplex virus proteins during AAV replication. PMID: 20573815
    8. AAV Rep inhibits herpes simplex virus type 1 at the level of DNA replication. PMID: 17314170
    9. Activation of the ATPase activity of Rep68 is associated with the formation of a protein-DNA oligomer PMID: 17474716

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  • 數(shù)據(jù)庫鏈接：

    KEGG: vg:4192013



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