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  4. Recombinant Actinosynnema mirum Pup--protein ligase (pafA)

Recombinant Actinosynnema mirum Pup--protein ligase (pafA)

  • 中文名稱：
    Recombinant Actinosynnema mirum Pup--protein ligase(pafA)
  • 貨號：
    CSB-YP513106AXJ
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Actinosynnema mirum Pup--protein ligase(pafA)
  • 貨號：
    CSB-EP513106AXJ
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Actinosynnema mirum Pup--protein ligase(pafA)
  • 貨號：
    CSB-EP513106AXJ-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Actinosynnema mirum Pup--protein ligase(pafA)
  • 貨號：
    CSB-BP513106AXJ
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Actinosynnema mirum Pup--protein ligase(pafA)
  • 貨號：
    CSB-MP513106AXJ
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    pafA
  • Uniprot No.：
  • 別名：
    pafA; Amir_2247Pup--protein ligase; EC 6.3.1.19; Proteasome accessory factor A; Pup-conjugating enzyme
  • 種屬：
    Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-452
  • 氨基酸序列
    MQRRIFGIET EFGVTCTFHG QRRLSPDEVA RYLFRRVVSW GRSSNVFLRN GSRLYLDVGS HPEYATAECD DLAQLVSHDK AGERILEDLL VDAERRLADE GIGGDIFLFK NNTDSAGNSY GCHENYLVAR AGEFSRIADV LLPFLVTRQL ICGAGKVLQT PRGAVYCLSQ RAEHIWEGVS SATTRSRPII NTRDEPHADA ERYRRLHVIV GDSNMSEVTT LLKVGSANLV LEMIEQGVQF RDFSLDNPIR AIREISHDLT GRRTVKLAGG KEASALDIQR EYYERAVEHV AKRSPDPMAE RVLELWGRTL DAIAAQDLSK IDREIDWAIK HRLLERYQAK HDMDLSNPRI AQLDLAYHDI RRGRGVFDLL QRKGQVERVT DDGEIEAAKD TPPQTTRAKL RGDFIAAAQA AGRDFTVDWV HLKLNDQAQR TVLCKDPFRA VDERVERLIA SL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.
  • 蛋白家族：
    Pup ligase/Pup deamidase family, Pup-conjugating enzyme subfamily
  • 數據庫鏈接：


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