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Recombinant Acinetobacter baumannii Beta-lactamase (NDM-1), partial

In Stock
  • 中文名稱:
    Recombinant Acinetobacter baumannii Beta-lactamase(NDM-1),partial
  • 貨號:
    CSB-EP761ABS
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP076174Ba could indicate that this peptide derived from E.coli-expressed Acinetobacter baumannii ndm-1.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP076174Ba could indicate that this peptide derived from E.coli-expressed Acinetobacter baumannii ndm-1.
  • 其他:

產品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    NDM-1
  • Uniprot No.:
  • 種屬:
    Acinetobacter baumannii
  • 蛋白長度:
    Partial
  • 來源:
    E.coli
  • 分子量:
    22.0kDa
  • 表達區域:
    164-222aa
  • 氨基酸序列
    AANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLG
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-SUMO-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產品描述:

    The DNA coding sequence translated into the Acinetobacter baumannii NDM-1 protein sequence (164-222aa) was fused with the N-terminal 6xHis-SUMO tag sequence to form the recombinant DNA, which was inserted into an expression vector. The reconstructed expression vector was transformed into the E.coli for follow-up expression. The product underwent purification to obtain the recombinant Acinetobacter baumannii NDM-1 protein with N-terminal 6xHis-SUMO tag. The SDS-PAGE analysis determined its purity higher than 90%. After electrophoresis, a 21 kDa protein band was observed on the gel.

    NDM-1 is a gene providing instructions for making a protein Beta-lactamase in Acinetobacter baumannii. This gene is a novel metallo-beta-lactamase (MBL) gene carried by some Enterobacteriaceae that induces resistance to most of the antibiotics. This gene was first describled in a Swedish patient hospitalized in India with an infection due to Klebsiella pneumoniae. NDM-1 makes bacteria resistant to a broad range of beta-lactam antibiotics, including the antibiotics of the carbapenem family (a mainstay for the treatment of antibiotic-resistant bacterial infections).

  • Datasheet & COA:
    Please contact us to get it.

產品評價



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