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  2. 蛋白
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  4. Recombinant 3-ketosteroid-9-alpha-hydroxylase oxygenase subunit (kshA)

Recombinant 3-ketosteroid-9-alpha-hydroxylase oxygenase subunit (kshA)

  • 中文名稱：
    結(jié)核分枝桿菌kshA重組蛋白
  • 貨號(hào)：
    CSB-YP304109MVZ
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    結(jié)核分枝桿菌kshA重組蛋白
  • 貨號(hào)：
    CSB-EP304109MVZ
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    結(jié)核分枝桿菌kshA重組蛋白
  • 貨號(hào)：
    CSB-EP304109MVZ-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    結(jié)核分枝桿菌kshA重組蛋白
  • 貨號(hào)：
    CSB-BP304109MVZ
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    結(jié)核分枝桿菌kshA重組蛋白
  • 貨號(hào)：
    CSB-MP304109MVZ
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    kshA
  • Uniprot No.：
  • 別名：
    kshA; Rv35263-ketosteroid-9-alpha-monooxygenase; oxygenase component; 3-ketosteroid-9-alpha-hydroxylase; oxygenase component; KSH; Androsta-1,4-diene-3,17-dione 9-alpha-hydroxylase; EC 1.14.15.30; Rieske-type oxygenase; RO
  • 種屬：
    Mycobacterium tuberculosis
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-386
  • 氨基酸序列
    MSTDTSGVGV REIDAGALPT RYARGWHCLG VAKDYLEGKP HGVEAFGTKL VVFADSHGDL KVLDGYCRHM GGDLSEGTVK GDEVACPFHD WRWGGDGRCK LVPYARRTPR MARTRSWTTD VRSGLLFVWH DHEGNPPDPA VRIPEIPEAA SDEWTDWRWN RILIEGSNCR DIIDNVTDMA HFFYIHFGLP TYFKNVFEGH IASQYLHNVG RPDVDDLGTS YGEAHLDSEA SYFGPSFMIN WLHNRYGNYK SESILINCHY PVTQNSFVLQ WGVIVEKPKG MSEEMTDKLS RVFTEGVSKG FLQDVEIWKH KTRIDNPLLV EEDGAVYQLR RWYEQFYVDV ADIKPEMVER FEIEVDTKRA NEFWNAEVEK NLKSREVSDD VPAEQH
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Involved in the degradation of cholesterol. Catalyzes the introduction of a 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD) to yield the 9alpha-hydroxy-1,4-androstadiene-3,17-dione (9OHADD) intermediate which spontaneously form 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (HSA) via the meta-cleavage of ring B with concomitant aromatization of ring A. KSH is also able to use 4-androstene-3,17-dione (AD), 3-oxo-23,24-bisnorcholesta-4-en-22-oate (4-BNC), 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate (1,4-BNC), 3-oxo-23,24-bisnorcholesta-4-en-22-oyl-coenzyme A thioester (4-BNC-CoA) and 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-coenzyme A thioester (1,4-BNC-CoA) as substrates.
  • 基因功能參考文獻(xiàn)：
    1. The deletion of either of these genes lead to rapid death of the microorganism in murine infection models and in macrophages, showing that kshA and kshB are essential factors for M. tuberculosis pathogenesis. PMID: 19906176
    2. Characterization of 3-ketosteroid 9{alpha}-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis PMID: 19234303
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: mtu:Rv3526

    STRING: 83332.Rv3526



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