Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Recombinant Monoclonal Antibody
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中文名稱：磷酸化-PAK4/PAK5/PAK6 (S474/S560/S602)重組抗體
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貨號：CSB-RA017408A474phHU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not)
All lanes Phospho-PAK4/PAK5/PAK6 antibody at 2.15μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64,75,80 KDa
Observed band size: 75,80 KDa -
Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with CSB-RA017408A474phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Recombinant Monoclonal Antibody（CSB-RA017408A474phHU）是一款高特異性抗體，靶向p21激活激酶家族成員PAK4、PAK5和PAK6的磷酸化修飾位點(diǎn)S474、S560及S602。該抗體經(jīng)ELISA、Western blot（WB）和免疫熒光（IF）嚴(yán)格驗(yàn)證，適用于檢測內(nèi)源性蛋白的磷酸化狀態(tài)。在WB實(shí)驗(yàn)中推薦使用1:500-1:5000的稀釋范圍，可清晰識(shí)別目標(biāo)條帶；IF實(shí)驗(yàn)推薦1:20-1:200稀釋度，能精準(zhǔn)定位蛋白在細(xì)胞內(nèi)的空間分布。PAK4/5/6作為Rho家族GTP酶下游效應(yīng)分子，參與調(diào)控細(xì)胞骨架重組、遷移及生存信號通路，其磷酸化修飾與激酶活性及功能調(diào)控密切相關(guān)。本抗體可為研究細(xì)胞極性建立、腫瘤侵襲轉(zhuǎn)移、神經(jīng)發(fā)育等領(lǐng)域的分子機(jī)制提供可靠工具，適用于基礎(chǔ)科研中細(xì)胞或組織樣本的蛋白表達(dá)水平分析、亞細(xì)胞定位研究及相關(guān)信號通路的功能探索。
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Uniprot No.：
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反應(yīng)種屬：Human
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免疫原：A synthesized peptide derived from Human Phospho-PAK4/PAK5/PAK6 (S474/S560/S602)
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：2D1
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IF 1:20-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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