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  4. Histone H2B type 1-K Recombinant Monoclonal Antibody

Histone H2B type 1-K Recombinant Monoclonal Antibody

  • 中文名稱：
    組蛋白 H2B type 1-K重組抗體
  • 貨號：
    CSB-RA010411A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Mouse brain tissue, Rat liver tissue, Rat lung tissue
      All lanes: Histone H2B type 1-K antibody at 0.16μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 14 KDa
      Observed band size: 14 KDa
    • IHC image of CSB-RA010411A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

產品詳情

  • 產品描述：
    CSB-RA010411A0HU為Histone H2B type 1-K重組單克隆抗體，靶向真核生物染色質核心組分組蛋白H2B家族成員，該蛋白通過與DNA及其他組蛋白協同組裝核小體，參與維持基因組穩定性和表觀遺傳調控。本抗體經多種實驗驗證，在Western Blot中展現出高特異性，可清晰檢測目標蛋白條帶，推薦使用1:5000-1:10000稀釋度；在免疫組化（IHC）中能有效定位細胞核內信號，建議稀釋度為1:50-1:500。此外，該抗體亦適用于ELISA檢測，為研究者提供多平臺兼容性。其低交叉反應性設計確保了對Histone H2B type 1-K亞型的高度選擇性，適用于染色質結構分析、表觀遺傳修飾研究及基因表達調控機制探索等科研領域，可為細胞周期、DNA損傷修復或轉錄調控相關實驗提供可靠工具。
  • Uniprot No.：
  • 基因名：
    HIST1H2BK
  • 別名：
    H2BC12 antibody; H2BFT antibody; HIRIP1 antibody; HIST1H2BK antibody; Histone H2B type 1-K antibody; H2B K antibody; HIRA-interacting protein 1 antibody
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    4G10
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:5000-1:10000
    IHC 1:50-1:500
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
  • 基因功能參考文獻：
    1. Histone H2B antimicrobial peptides exhibit antibacterial activity and contribute to the formation of the antimicrobial barrier of the colonic epithelium PMID: 15019208
  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H2B family
  • 數據庫鏈接：

    HGNC: 13954

    OMIM: 615045

    KEGG: hsa:85236

    STRING: 9606.ENSP00000349430

    UniGene: Hs.437275



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