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FNTB Recombinant Monoclonal Antibody

  • 中文名稱：
    FNTB重組抗體
  • 貨號：
    CSB-RA267691A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Rat brain tissue
      All lanes: FNTB antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 49, 44 kDa
      Observed band size: 49 kDa
    • IHC image of CSB-RA267691A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Immunoprecipitating FNTB in K562 whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-RA267691A0HU in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-RA267691A0HU(2μg)+ K562 whole cell lysate(500μg)
      Lane 3: K562 whole cell lysate (10μg)
  • 其他：

產品詳情

  • 產品描述：
    CSB-RA267691A0HU FNTB重組單克隆抗體是針對法尼基轉移酶β亞基（FNTB）研發的高特異性檢測工具。FNTB作為法尼基轉移酶復合體的關鍵催化亞基，通過介導Ras等蛋白的法尼基化修飾參與細胞增殖與信號轉導調控，其異常表達與多種腫瘤發生發展密切相關。本產品經多種平臺嚴格驗證：在Western Blot中可清晰識別天然及重組FNTB蛋白，呈現單一特異性條帶；免疫組化實驗顯示其在組織切片中具有精準的亞細胞定位能力；免疫共沉淀驗證證實其能高效富集內源性FNTB及其互作蛋白。推薦使用稀釋度覆蓋WB（1:500-5000）、IHC（1:50-200）及IP（1:200-1000）等多種實驗體系，適用于癌癥分子機制研究、蛋白質翻譯后修飾分析以及靶向法尼基化通路藥物的體外篩選等科研場景。該抗體具有批次穩定性高、交叉反應性低的特點，為探索FNTB在細胞信號網絡中的功能及其病理學意義提供了可靠工具。
  • Uniprot No.：
  • 基因名：
    FNTB
  • 別名：
    Protein farnesyltransferase subunit beta (FTase-beta) (EC 2.5.1.58) (CAAX farnesyltransferase subunit beta) (Ras proteins prenyltransferase subunit beta), FNTB
  • 反應種屬：
    Human, Rat
  • 免疫原：
    A synthesized peptide derived from human FNTB
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    5H4
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IP 1:200-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Essential subunit of the farnesyltransferase complex. Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X.
  • 蛋白家族：
    Protein prenyltransferase subunit beta family
  • 數據庫鏈接：

    HGNC: 3785

    OMIM: 134636

    KEGG: hsa:100529261

    STRING: 9606.ENSP00000246166

    UniGene: Hs.325531



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