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ATF5 Recombinant Monoclonal Antibody

  • 中文名稱：
    ATF5重組抗體
  • 貨號：
    CSB-RA180846A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, Rat brain tissue
      All lanes: ATF5 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 31 kDa
      Observed band size: 35 kDa
    • IHC image of CSB-RA180846A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA180846A0HU ATF5重組單克隆抗體是針對人源轉(zhuǎn)錄激活因子5（ATF5）開發(fā)的高特異性科研用試劑。ATF5屬于ATF/CREB轉(zhuǎn)錄因子家族成員，廣泛參與細胞增殖、分化和應激反應的調(diào)控，尤其在神經(jīng)發(fā)育及腫瘤發(fā)生相關通路中發(fā)揮重要作用。本抗體經(jīng)多種實驗驗證，在Western Blot中可清晰檢測內(nèi)源性ATF5蛋白條帶，在免疫組化實驗中能精準定位組織切片中的靶蛋白表達，同時適用于ELISA檢測體系。推薦使用稀釋度為WB 1:500-1:5000、IHC 1:50-1:200，其優(yōu)化的反應體系可滿足不同樣本類型的檢測需求。該產(chǎn)品適用于研究ATF5在神經(jīng)干細胞分化、腫瘤細胞異常增殖等過程中的分子機制，以及通過蛋白水平分析其在正常組織與病變組織中的表達差異，為神經(jīng)生物學、腫瘤微環(huán)境研究及細胞應激反應機制探索提供可靠工具。
  • Uniprot No.：
  • 基因名：
    ATF5
  • 別名：
    Cyclic AMP-dependent transcription factor ATF-5 (cAMP-dependent transcription factor ATF-5) (Activating transcription factor 5) (Transcription factor ATFx), ATF5, ATFX
  • 反應種屬：
    Human, Rat
  • 免疫原：
    A synthesized peptide derived from human ATF5
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    9D8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate. Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation. Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes. May be involved in osteogenic differentiation. Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1. Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent. Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver. In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase. May act as a negative regulator of IL1B transduction pathway in liver. Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members. Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation. Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material.
  • 基因功能參考文獻：
    1. Data suggest that ATF5 is modified by SUMO2/3 at a conserved SUMO-targeting consensus site; this SUMOylation of ATF5 appears to be required for transport of ATF5 to centrosome. (ATF5 = activating transcription factor-5; SUMO = small ubiquitin-like modifier) PMID: 29326161
    2. Study reports that reduced levels of ATF5 in brain of Huntington's disease patients, probably due to its sequestration into the characteristic PolyQ containing neuronal inclusion bodies, correlates with decreased levels of the antiapoptotic protein MCL1, a transcriptional target of ATF5. Also provides evidence of decreased ATF5 being deleterious by rendering neurons more vulnerable to polyQ-induced apoptosis. PMID: 28861715
    3. ATF5 expression can rescue UPR(mt) signaling in atfs-1-deficient worms requiring the same UPR(mt) promoter element identified in C. elegans. Furthermore, mammalian cells require ATF5 to maintain mitochondrial activity during mitochondrial stress and promote organelle recovery. Combined, these data suggest that regulation of the UPR(mt) is conserved from worms to mammals. PMID: 27426517
    4. Our results suggest that ATF5 promotes invasion by inducing the expression of integrin-alpha2 and integrin-beta1 in several human cancer cell lines. PMID: 27125458
    5. This study provides the first evidence that the methylation level of ATF5 decreased, and its mRNA expression was evidently up-regulated in glioma. PMID: 26365117
    6. These results suggest that the hepatic functions of the human iPS-HLCs could be enhanced by ATF5, c/EBPalpha, and PROX1 transduction. PMID: 26679606
    7. Activating transcription factor 5 enhances radioresistance and malignancy in cancer. PMID: 25682872
    8. Data show that ATF5 is an essential structural protein that is required for the interaction between the mother centriole and the pericentriolar material. PMID: 26213385
    9. Low expression level of ATF5 in hepatocellular carcinoma indicated aggressive tumor behavior and predicted a worse clinical outcome. PMID: 25294425
    10. Report a global loss of 5hmC identified three new genes (ECM1, ATF5, and EOMES) with potential anti-cancer functions that may promote the understanding of the molecular mechanisms of hepatocellular carcinoma development and progression. PMID: 25517360
    11. the TAK1-NLK pathway is a novel regulator of basal or IL-1beta-triggered C/EBP activation though stabilization of ATF5 PMID: 25512613
    12. ATF5 promotes the proliferation of HSV-1 via a potential mechanism by which ATF5 enhances the transcription of viral genes during the course of an HSV-1 infection PMID: 24302293
    13. N-terminal hydrophobic amino acids play an important role in the regulation of ATF5 protein expression in IL-1beta-mediated immune response and that ATF5 is a negative regulator for IL-1beta-induced expression of SAA1 and SAA2 in HepG2 cells. PMID: 24379400
    14. The 5'-untranslated region regulates ATF5 mRNA stability via nonsense-mediated mRNA decay in response to environmental stress. PMID: 23876217
    15. demonstrated that interference with the function of ATF5 could markedly increase the apoptosis of ovarian cancer cells and identified B-cell leukemia lymphoma-2 as an ATF5-targeted apoptosis-related gene PMID: 23018213
    16. The evidence suggests a role for ATF5 in the regulation of osteogenic differentiation in adipose-derived stem cells. PMID: 22442021
    17. a mechanistic link between elevated NPM1 expression and depressed ATF5 in HCC and suggests that regulation of ATF5 by NPM1 plays an important role in the proliferation and survival of HCC. PMID: 22528486
    18. ATF5 polymorphisms influence ATF function and response to treatment in children with childhood acute lymphoblastic leukemia. PMID: 21972289
    19. coordinated actions by ATF5, p300, Elk-1, and ERK/mitogen-activated protein kinase are essential for ATF5-dependent Egr-1 activation and cell proliferation and survival PMID: 21791614
    20. an essential role for HSP70 in maintaining high levels of ATF5 expression in glioma cells and support the conclusion that ATF5 is an important substrate protein of HSP70 that mediates HSP70-promoted cell survival in glioma cells. PMID: 21521685
    21. BCL-2 is an essential mediator for the cancer-specific cell survival function of ATF5 in glioblastoma and breast cancer cells PMID: 21212266
    22. our studies not only provided molecular basis of ATF5 transcriptional regulation, but also identified ATF5 as a target gene of EBF1 transcription factor. PMID: 20423929
    23. activating transcription factor 5 (ATF5) is a new interacting partner of cyclin D3 PMID: 15358120
    24. recruitment of ATFx to the HTLV-1 LTR serves to link viral transcription with critical events in cellular homeostasis PMID: 15890932
    25. Overexpression of the bZIP protein ATF5, a transcriptional activator, stimulates asparagine synthetase promoter/reporter gene transcription via the nutrient-sensing response unit. PMID: 16164412
    26. The widespread expression of ATF5 in glioblastomas PMID: 16170340
    27. ATF5 increases cisplatin-induced apoptosis through up-regulation of Cyclin D3 transcription, which elicits survival signals in HeLa cells PMID: 16300731
    28. Contribution of common variations of ATF4 and ATF5 to the pathophysiology of bipolar disorder may be minimal if any. PMID: 17346882
    29. translation of ATF5 is regulated by the alternative 5'-UTR region of its mRNA, and ATF5 may play a role in protecting cells from amino acid limitation or arsenite-induced oxidative stress PMID: 18055463
    30. ATF4 contributes to basal ATF5 transcription, and eIF2 kinases direct the translational expression of multiple transcription regulators by a mechanism involving delayed translation reinitiation PMID: 18195013
    31. ATF5 is abundant in the liver, activates CYP2B6, and cooperates with the constitutive androstane receptor in sustaining the hepatic-specific expression of this P450 in human hepatocytes and hepatoma cells. PMID: 18332083
    32. cisplatin increased ATF5 protein expression via preventing its ubiquitin-dependent degradation, which might be associated with its promoting the nucleus-to-cytoplasm translocation of E2 ubiquitin-conjugating enzyme Cdc34 PMID: 18458088
    33. Loss of ATF5 is associated with hepatocellular carcinoma PMID: 18701499
    34. The down-regulation of the SAP gene by ATF5 may represent a common mechanism for the pathogenesis of Hemophagocytic syndrome that is associated with either Epstein-Barr virus infection or immune disorders with dysregulated T-cell activation. PMID: 18832568
    35. These results indicate that ATF5 is targeted for degradation by the ubiquitin-proteasome pathway, and that cadmium slows the rate of ATF5 degradation via a post-ubiquitination mechanism. PMID: 19285020
    36. Identified a novel ATF5 consensus DNA binding sequence. Demonstrate in C6 glioma and MCF-7 breast cancer cells that ATF5 occupies this sequence and that ATF5 activates reporter gene expression driven by this site. PMID: 19531563

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  • 亞細胞定位：
    Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    BZIP family
  • 組織特異性：
    Widely expressed with higher expression levels in liver.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 790

    OMIM: 606398

    KEGG: hsa:22809

    STRING: 9606.ENSP00000396954

    UniGene: Hs.9754



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