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  4. ZBTB7A Recombinant Monoclonal Antibody

ZBTB7A Recombinant Monoclonal Antibody

  • 中文名稱：
    ZBTB7A Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA063930A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: A431 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), HT-29 whole cell lysate(30μg), THP-1 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), HeLa whole cell lysate(30μg), A549 whole cell lysate(30μg)
      All lanes: ZBTB7A antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 61 kDa
      Observed band size: 70 kDa
      Exposure time：2min
    • IHC image of CSB-RA063930A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA063930A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of MCF-7 cell with CSB-RA063930A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing BxPC-3 cells stained with CSB-RA063930A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    ZBTB7A
  • 別名：
    Zinc finger and BTB domain-containing protein 7A (Factor binding IST protein 1) (FBI-1) (Factor that binds to inducer of short transcripts protein 1) (HIV-1 1st-binding protein 1) (Leukemia/lymphoma-related factor) (POZ and Krueppel erythroid myeloid ontogenic factor) (POK erythroid myeloid ontogenic factor) (Pokemon) (TTF-I-interacting peptide 21) (TIP21) (Zinc finger protein 857A), ZBTB7A, FBI1 LRF ZBTB7 ZNF857A
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide from human ZBTB7A protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    8D5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation. Directly and specifically binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' and represses transcription both by regulating the organization of chromatin and through the direct recruitment of transcription factors to gene regulatory regions. Negatively regulates SMAD4 transcriptional activity in the TGF-beta signaling pathway through these two mechanisms. That is, recruits the chromatin regulator HDAC1 to the SMAD4-DNA complex and in parallel prevents the recruitment of the transcriptional activators CREBBP and EP300. Collaborates with transcription factors like RELA to modify the accessibility of gene transcription regulatory regions to secondary transcription factors. Also directly interacts with transcription factors like SP1 to prevent their binding to DNA. Functions as an androgen receptor/AR transcriptional corepressor by recruiting NCOR1 and NCOR2 to the androgen response elements/ARE on target genes. Thereby, negatively regulates androgen receptor signaling and androgen-induced cell proliferation. Involved in the switch between fetal and adult globin expression during erythroid cells maturation. Through its interaction with the NuRD complex regulates chromatin at the fetal globin genes to repress their transcription. Specifically represses the transcription of the tumor suppressor ARF isoform from the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation. Regulates chondrogenesis through the transcriptional repression of specific genes via a mechanism that also requires histone deacetylation. Regulates cell proliferation through the transcriptional regulation of genes involved in glycolysis. Involved in adipogenesis through the regulation of genes involved in adipocyte differentiation. Plays a key role in the differentiation of lymphoid progenitors into B and T lineages. Promotes differentiation towards the B lineage by inhibiting the T-cell instructive Notch signaling pathway through the specific transcriptional repression of Notch downstream target genes. Also regulates osteoclast differentiation. May also play a role, independently of its transcriptional activity, in double-strand break repair via classical non-homologous end joining/cNHEJ. Recruited to double-strand break sites on damage DNA, interacts with the DNA-dependent protein kinase complex and directly regulates its stability and activity in DNA repair. May also modulate the splicing activity of KHDRBS1 toward BCL2L1 in a mechanism which is histone deacetylase-dependent and thereby negatively regulates the pro-apoptotic effect of KHDRBS1.
  • 基因功能參考文獻：
    1. provide the evidence for the regulatory role of miR-106b in HCC, which is causally linked to targeting of Zbtb7a PMID: 29975452
    2. The specific association of ZBTB7A mutations with t(8;21) rearranged acute myeloid leukaemia points towards leukemogenic cooperativity between mutant ZBTB7A and the RUNX1/RUNX1T1 fusion protein has been reported. PMID: 27252013
    3. ZBTB7A is increased in cisplatin-resistant osteosarcoma cells and elevated ZBTB7A inhibits cisplatin-induced apoptosis by repressing LINC00473 expression. PMID: 28942243
    4. Our data suggest that ZBTB7A is involved in the development and progression of colorectal carcinoma PMID: 29715100
    5. Results indicate the reverse association between the expression of ZBTB7A and the tumorigenicity of nasopharyngeal carcinoma (NPC). PMID: 29699474
    6. Alveolar macrophages deficient in Zbtb7a failed to induce antibody and T cell responses. PMID: 28701473
    7. Variants of ZBTB7A (LRF) and its beta-globin gene cluster binding motifs in sickle cell anemia/ PMID: 27282567
    8. This analysis complements the understanding of the mutational landscape of core binding factor acute myeloid leukemia, and has led to the identification of novel ZBTB7A mutations in t(8;21) acute myeloid leukemia subgroup. PMID: 26968532
    9. Lymphoma-related factor mediates the protease-activated receptor 2-suppressed p53 expression in the cancer cells and binds DAP5 to interfere with the gene transcription of p53 in colon cancer cells. PMID: 28849590
    10. Our study uncovers the loss-of-function mutation in ZBTB7A as a novel mechanism causing elevated glycolysis in human cancer, which carries important therapeutic implication. PMID: 26455326
    11. Mutation in ZBTB7A is associated with core-binding factor acute myeloid leukemia. PMID: 27798625
    12. Zbtb7a knockdown by siRNA suppressed cell proliferation and induced G1 cell cycle arrest and apoptosis in lung cancer cells. Furthermore, miR-125a expression showed a negative correlation with Zbtb7a expression in non-small cell lung cancer tissues. PMID: 26713860
    13. Data suggest that microRNA miR-520e directly targets to the 3'-untranslated region (3'-UTR) of zinc finger and BTB domain containing 7A protein (Zbtb7a). PMID: 28242196
    14. CCAT2 promotes tumorigenesis by over-expression of Pokemon. PMID: 28088736
    15. High FBI-1 expression is associated with hepatocellular carcinoma. PMID: 27492460
    16. there is an auto-regulatory circuit consisting of miR-125 and Pokemon, which promotes the progression of HCC and may be a promising therapeutic target in clinical HCC treatment PMID: 26227218
    17. Pokemon was found to enhance the migration and invasion of hepatocellular carcinoma by increasing MEF2D expression PMID: 26797719
    18. Pokemon was significantly downregulated in OSCC. PMID: 25439053
    19. ZBTB7A directly binds to the promoter and transcriptionally represses the expression of MCAM, establishing ZBTB7A as a bona fide transcriptional repressor of MCAM PMID: 25995384
    20. MEF2D was also found to increase the transcription of Pokemon by binding myocyte enhancer factor 2 (MEF2) sites within its promoter region, that can promote Hepatocellular carcinoma invasion PMID: 26164003
    21. this study found that LRF/ZBTB7A transcription factor occupies fetal gamma-globin genes and maintains the nucleosome density necessary for gamma-globin gene silencing in adults, and that LRF confers its repressive activity through a NuRD repressor complex independent of the fetal globin repressor BCL11A. PMID: 26816381
    22. p38beta is a novel regulatory target of the transcription factor Pokemon and positively regulated by Pokemon in hepatic cells. PMID: 23807508
    23. Taken together, all these data suggest that Pokemon is a new partner of Smad4 and plays a negative role in TGF-beta pathway. PMID: 25514493
    24. Pokemon enhanced the oncogenesis of colorectal cancer by promoting cell proliferation, cell cycle progression and anti-apoptosis activity. PMID: 25367850
    25. our findings suggest that TGF-b1 may inhibit the expression of Pokemon, b-catenin, and E-cadherin. The high expression of TGF-b1 leads to an increase in the phenotype and apical-base polarity of epithelial cells PMID: 25722217
    26. a potential regulatory role of FBI-1 in transcription factor activity of ETS-1 PMID: 24857950
    27. Pokemon expression of NP69 and C666-1 obviously varied from mRNA to protein. PMID: 24377524
    28. The mRNA expression level of Pokemon in colorectal adenocarcinoma was significantly higher than that in adjacent tumor specimens. The positive expression ratio of Pokemon protein in colorectal cancer was significantly higher than in the adjacent tissues. PMID: 24175766
    29. An unexpected function for FBI-1 in splicing modulation with a direct impact on cell survival. PMID: 24514149
    30. ZBTB7A, a member of the POK (POZ/BTB and Kruppel) transcription repressor family, directly binds to the promoter and represses the transcription of critical glycolytic genes, including GLUT3, PFKP, and PKM PMID: 25184678
    31. For the first time, we show that LRF/Pokemon is predominantly expressed in nodular lymphocyte-predominant Hodgkin lymphoma with low to absent NOTCH1 protein expression PMID: 24326827
    32. Investigated the functions of Pokemon in the carcinogenesis and development of bladder cancer and observed the inhibitory effects of siRNA expression vector on Pokemon in bladder cancer cells. PMID: 24393848
    33. Pokemon might serve as an important mediator of crosstalk between intrinsic and extrinsic apoptotic pathways in HCC cel PMID: 23874836
    34. Data indicate that ZBTB7A is identified as a strong candidate for regulation of thymic insulin expression. PMID: 23911422
    35. our results may help better understand the role of Pokemon in hepatocellular carcinoma and promote the clinical applications PMID: 24261083
    36. Knockdown of Pokemon protein expression inhibits hepatocellular carcinoma cell proliferation by suppression of AKT activity. PMID: 23924858
    37. ZBTB7A is a context-dependent cancer gene that can act as an oncogene in some contexts but also has oncosuppressive-like activity in PTEN-null tumors. PMID: 23727861
    38. Pokemon promotes cell proliferation and migration in hepatocellular carcinoma and accelerates tumor development. PMID: 23300578
    39. Studies indicate that LRF regulates hematopoiesis by playing specific roles in different cell lineages. PMID: 23396304
    40. Pokemon activates the expression of both p65 and IkappaBalpha by sequence-specific binding to their promoters and plays a dual role in regulating NF-kappaB signaling. PMID: 23054188
    41. The oncogene LRF is a survival factor in chondrosarcoma and contributes to tumor malignancy and drug resistance. PMID: 22847180
    42. The activities of CDK2 and E2F4 promoters were suppressed by the modulation of Zbtb7 levels and that Zbtb7 represses promoter activities through a mechanism involving direct binding of Zbtb7 to the promoters. PMID: 22447046
    43. Pokemon promotes breast cancer progression by upregulating survivin expression and may be a potential target for the treatment of this malignancy PMID: 21392388
    44. Depletion of Pokemon inhibited proliferation of HepG(2) or induced apoptosis. PMID: 21985851
    45. The current findings indicated that FBI-1 plays an important role in HCC carcinogenesis and chemotherapy tolerance PMID: 21713761
    46. siRNA of Pokemon was applied to inhibit the expression of Pokemon and NF-kappa B p65 and apoptotic rate was determined by flow cytometric analysis PMID: 21771706
    47. The aim of this study was to examine the regulation of LRF expression in human prostate cells. PMID: 21640721
    48. FBI-1/OCZF/LRF regulates osteoclast formation and apoptosis in vivo, and may become a useful marker and target in treating disorders leading to reduced bone density, including chronic arthritis. PMID: 21590684
    49. FBI-1 functions as a novel androgen receptor co-repressor in prostate cancer cells. PMID: 20812024
    50. The expression of LRF was evaluated in benign prostate hyperplastic (BPH) and prostate cancer (PC) tissues. PMID: 21251909

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  • 亞細胞定位：
    Nucleus.
  • 組織特異性：
    Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas,
  • 數(shù)據(jù)庫鏈接：

    HGNC: 18078

    OMIM: 605878

    KEGG: hsa:51341

    STRING: 9606.ENSP00000323670

    UniGene: Hs.591384



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