在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

YWHAE Recombinant Monoclonal Antibody

  • 中文名稱:
    YWHAE Recombinant Monoclonal Antibody
  • 貨號:
    CSB-RA115871A0HU
  • 規(guī)格:
    ¥1320
  • 圖片:
    • Western Blot
      Positive WB detected in: Hela whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), A431 whole cell lysate(30μg), HEK293T whole cell lysate(30μg), Jurkat whole cell lysate(30μg), U-937 whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg), Mouse brain tissue lysate(30μg)
      All lanes: 14-3-3 epsilon antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 29 kDa
      Observed band size: 29 kDa
      Exposure time:1min
    • IHC image of CSB-RA115871A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing Hela cells stained with CSB-RA115871A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他:

產(chǎn)品詳情

  • Uniprot No.:
  • 基因名:
  • 別名:
    14-3-3 protein epsilon (14-3-3E), YWHAE
  • 反應(yīng)種屬:
    Human, Mouse
  • 免疫原:
    A synthesized peptide from human YWHAE protein
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標(biāo)記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    Rabbit IgG
  • 純化方式:
    Affinity-chromatography
  • 克隆號:
    8D9
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式:
    Liquid
  • 應(yīng)用范圍:
    ELISA, WB, IHC, FC
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能:
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm.
  • 基因功能參考文獻(xiàn):
    1. results support YWHAE being a risk gene for Major Depressive Disorder in the Han Chinese population. PMID: 28414084
    2. YWHAE silencing induces cell proliferation, invasion and migration through the up-regulation of CDC25B and MYC in gastric cancer cells. PMID: 27863420
    3. we report recurrent BCOR exon 16 internal tandem duplications and YWHAE-NUTM2B fusions in half of infantile soft tissue undifferentiated round cell sarcoma and most primitive myxoid mesenchymal tumor of infancy cases, but not in other pediatric sarcomas. PMID: 26945340
    4. For metastatic YWHAE-rearranged HG-ESS, prolonged disease control following diagnosis was seen, with notable responses to anthracycline-based therapy. This emphasizes the need for appropriate molecular testing of uterine mesenchymal malignancies. PMID: 28390819
    5. phosphorus NMR and time-resolved tryptophan fluorescence measurements suggest that 14-3-3zeta interacts with the kinase domain of ASK1 in close proximity to its active site, thus indicating this interaction might block its accessibility and/or affect its conformation. PMID: 27514745
    6. The 14-3-3 family is dysregulated in schizophrenia, perhaps owing to specific regulatory mechanisms; the expression of the 14-3-3 epsilon, theta and zeta isoforms could be useful indicators of disease severity. PMID: 27030512
    7. Studies show that patients with clear cell sarcoma of the kidney (CCSK) and the fusion YWHAE-NUTM2B/E were relatively young, had low tumor volumes, and did not present with stage I disease which fail to identify an explicit clinical phenotype. PMID: 26542179
    8. Data show that the NS3 protein of dengue virus bound to 14-3-3 epsilon protein (14-3-3varepsilon) and prevented translocation of retinoic acid-inducible gene-I protein (RIG-I) to the adaptor MAVS protein and thereby blocked antiviral signaling. PMID: 26998762
    9. Report mutually exclusive BCOR internal tandem duplications and YWHAE-NUTM2 fusions in clear cell sarcoma of kidney. PMID: 27000436
    10. CEP131 is the key regulatory target of MK2 and 14-3-3 in centriolar satellite remodeling. PMID: 26616734
    11. 14-3-3epsilon might directly bind to CD13, which transmits its signal in chondrocytes to induce a catabolic phenotype similar to that observed in osteoarthritis. PMID: 26208633
    12. DAPK2-induced apoptosis is negatively regulated by Akt and 14-3-3 proteins. PMID: 26047703
    13. These data demonstrate a novel role for HDAC6 in controlling 14-3-3zeta binding activity. PMID: 25770209
    14. YWHAE-FAM22 rearrangement is associated with endometrial stromal sarcomas. PMID: 25244606
    15. Results suggest a specific role for YWHAE single nucleotide polymorphisms in the pathophysiology of schizophrenia. PMID: 25105667
    16. Cleft lip/palate has been found associated with 17p13.3 duplication involving a single candidate gene (YWHAE) in a father and his son. PMID: 23901910
    17. Data show that two isoforms of the 14-3-3 family, 14-3-3epsilon and 14-3-3 gamma, can stabilize Cdt2 independent of each other when overexpressed. PMID: 25154416
    18. These results suggest a possible role for the YWHAE genotype in the early development of the OFC sulcogyral pattern in patients with schizophrenia. PMID: 24561237
    19. data indicate that 14-3-3 is required for Bim expression and that it has an anti-cancer effect under pERK1/2 inhibition in 143B cells PMID: 24536031
    20. for endometrial sarcoma cases with high-grade morphology we recommend to test for YWHAE rearrangements by FISH break-apart PMID: 23599159
    21. YWHAE polymorphisms may be a genetic risk factor for HIV-associated neurocognitive disorder (HAND). PMID: 23982958
    22. Data indicate that 14-3-3 zeta, gamma, epsilon, and tau isoforms but not the sigma protein hydrolyze ATP. PMID: 24269678
    23. Carriers of the YWHAE*C allele of rs3752826 polymorphism have increased risk of suicidal behavior. PMID: 24450199
    24. findings suggest that FAK expression is correlated with and upregulated by 14-3-3epsilon via activation of NFkappaB PMID: 22934705
    25. interaction between NAV2 and 14-3-3epsilon PMID: 24161943
    26. 14-3-3epsilon associates with TAK1 in a phosphorylation-dependent manner to determine the cell fate of Bleomycin-treated HCC cells PMID: 23472066
    27. YWHAE gene duplication is associated with developmental, behavioral and brain abnormalities. PMID: 23813913
    28. 14-3-3epsilon may act as an important regulator in modulating tumor metastasis by promoting epithelial-mesenchymal transition as well as cell migration. PMID: 23483955
    29. The subcellular localization of full-length human MLF1 is 14-3-3epsilon-independent. PMID: 23271436
    30. The ERK/MAPK pathway is involved in the pathogenesis of gastric cancer; RKIP and 14-3-3epsilon exert an opposite effect on this pathway and the cells possibly via both direct and indirect reactions with the elements in this pathway. PMID: 22899242
    31. the -458G>T YWHAE variant contributes to the abnormal myocardial morphogenesis characteristic of LVNC as well as abnormal brain development, and implicate YWHAE as a novel candidate gene in pediatric cardiomyopathies. PMID: 23266643
    32. YWHAE gene contribute to the phenotype of small 17p13.3 chromosomal duplication in Miller-Dieker syndrome PMID: 23035971
    33. results suggest that YWHAE does play a major role in bipolar disorder in the Han Chinese population. PMID: 23140658
    34. These results showed that IgE is produced during malaria, and that the negative correlation between levels of IgE to 14-3-3 epsilon protein and parasitemia in asymptomatic malaria supports a role for these IgE molecules in defense mechanisms. PMID: 22984113
    35. Data obtained, which have never previously been reported, were used to modify the published 14-3-3 crystal structure using molecular modeling. PMID: 22580067
    36. When evaluating high-grade uterine sarcomas, cyclin D1 can be included in the immuno-histochemical panel as an indicator of YWHAE-FAM22endometrial stromal sarcoma. PMID: 22982899
    37. results indicate that the dosage effect of YWHAE varies from severe to very mild structural brain abnormalities, and suggest that the expression of YWHAE is associated with a complex mechanism of neuronal development PMID: 22887762
    38. 14-3-3epsilon is essential for the stable interaction of RIG-I with TRIM25, which facilitates RIG-I ubiquitination and initiation of innate immunity against hepatitis C virus and other pathogenic RNA viruses. PMID: 22607805
    39. Tumors with YWHAE-FAM22 rearrangements constitute a distinct group of endometrial stromal sarcoma (ESS), which is associated with high-grade morphology and aggressive clinical behavior compared to JAZF1 ESS. PMID: 22456610
    40. Collectively, these results identify a novel locus of apoptosomal regulation wherein MAPK signalling promotes Rsk-catalysed Apaf-1 phosphorylation and consequent binding of 14-3-3varepsilon, resulting in decreased cellular responsiveness to cytochrome c. PMID: 22246185
    41. Data present the high-resolution crystal structure of this binding motif [MLF1(29-42)pSer34] in complex with 14-3-3epsilon and analyse the interaction with isothermal titration calorimetry. PMID: 22151054
    42. Fluorescence in situ hybridization analysis demonstrated absolute specificity of YWHAE-FAM22A/B genetic rearrangement for high-grade endometrial stromal sarcoma. PMID: 22223660
    43. YWHAE does not play a major role in schizophrenia, major depressive disorder or bipolar disorder in the Han Chinese populatio PMID: 21184166
    44. Data suggest a regulatory circuitry involving Smad4 dependent up-regulation of KRT23 (directly or indirectly) which in turn modulates the interaction between KRT23 and 14-3-3epsilon leading to a cytoplasmic sequestration of 14-3-3epsilon. PMID: 21492476
    45. Results suggest that ERK and p38 MAPK regulate subcellular localization of Prdx6 by activation of 14-3-3epsilon as a chaperone protein, resulting in its translocation to acidic organelles. PMID: 21346153
    46. Overexpression of 14-3-3epsilon predicts tumour metastasis and poor survival in hepatocellular carcinoma PMID: 21401702
    47. For the first time, we found that TNF-alpha stimulation enhances the interactions between 14-3-3 epsilon and some key components in the mitogen-activated protein kinase (MAPK) signal module which is located at the immediate upstream of NF-kappaB. PMID: 20462248
    48. The 14-3-3 epsilon, zeta and theta may be involved in tumorigenesis of meningioma and be efficient markers for predicting the degree of malignancy in meningioma. PMID: 20388496
    49. Decreased expression of 14-3-3epsilon in contributes to the initiation and progression of laryngeal carcinoma by affecting apoptosis and invasion. PMID: 20565895
    50. the identification of a novel splicing variant of human 14-3-3 epsilon (14-3-3 epsilon sv), derived from a novel exon 1' insertion is reported. PMID: 20417184

    顯示更多

    收起更多

  • 亞細(xì)胞定位:
    Nucleus. Cytoplasm. Melanosome.
  • 蛋白家族:
    14-3-3 family
  • 數(shù)據(jù)庫鏈接:

    HGNC: 12851

    OMIM: 605066

    KEGG: hsa:7531

    STRING: 9606.ENSP00000264335

    UniGene: Hs.513851



主站蜘蛛池模板: 牛牛视频一区二区三区| 男女做爰猛烈啪啪吃奶动 | 无码国产色欲xxxx视频| 国产亚洲精品久久久久久大师| 揉捏奶头高潮呻吟视频| 色欲综合久久躁天天躁蜜桃 | 亚洲成a人v电影在线观看| 久久经精品久久精品免费观看 | 99久久国产综合精品女图图等你| 天堂最新版在线www| 亚洲爆乳少妇无码激情| 亚洲国产欧美国产综合一区| 国产av夜夜欢一区二区三区| 国产爆乳无码av在线播放| 亚洲中文字慕日产2021| 免费精品国产自在| 国产av一区二区三区| 免费观看激色视频网站| 2020最新国产在线不卡a| 成人伊人亚洲人综合网| 少妇裸交aa大片| 日本内射精品一区二区视频| 亚洲另类成人小说综合网| 免费无码黄十八禁网站在线观看 | 老子午夜精品无码不卡| 国产精品午夜福利不卡| 午夜免费啪视频观看视频| 肥臀熟女一区二区三区| 亚洲色偷偷色噜噜狠狠99网| 亚洲一区二区无码偷拍| 手机看片精品国产福利| 综合久久给合久久狠狠狠97色| 日韩一区二区在线观看视频| av无码天堂一区二区三区| 加勒比中文无码久久综合色| 中国娇小与黑人巨大交| 免费看无码毛视频成片| 日韩精品一区二区大桥未久| 午夜激成人免费视频在线观看| 欧美日本国产va高清cabal| 成 人色 网 站 欧美大片在线观看|