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YWHAE Recombinant Monoclonal Antibody

  • 中文名稱:
    YWHAE Recombinant Monoclonal Antibody
  • 貨號:
    CSB-RA115871A0HU
  • 規(guī)格:
    ¥1320
  • 圖片:
    • Western Blot
      Positive WB detected in: Hela whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), A431 whole cell lysate(30μg), HEK293T whole cell lysate(30μg), Jurkat whole cell lysate(30μg), U-937 whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg), Mouse brain tissue lysate(30μg)
      All lanes: 14-3-3 epsilon antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 29 kDa
      Observed band size: 29 kDa
      Exposure time:1min
    • IHC image of CSB-RA115871A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing Hela cells stained with CSB-RA115871A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他:

產(chǎn)品詳情

  • Uniprot No.:
  • 基因名:
  • 別名:
    14-3-3 protein epsilon (14-3-3E), YWHAE
  • 反應(yīng)種屬:
    Human, Mouse
  • 免疫原:
    A synthesized peptide from human YWHAE protein
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標(biāo)記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    Rabbit IgG
  • 純化方式:
    Affinity-chromatography
  • 克隆號:
    8D9
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式:
    Liquid
  • 應(yīng)用范圍:
    ELISA, WB, IHC, FC
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能:
    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm.
  • 基因功能參考文獻(xiàn):
    1. results support YWHAE being a risk gene for Major Depressive Disorder in the Han Chinese population. PMID: 28414084
    2. YWHAE silencing induces cell proliferation, invasion and migration through the up-regulation of CDC25B and MYC in gastric cancer cells. PMID: 27863420
    3. we report recurrent BCOR exon 16 internal tandem duplications and YWHAE-NUTM2B fusions in half of infantile soft tissue undifferentiated round cell sarcoma and most primitive myxoid mesenchymal tumor of infancy cases, but not in other pediatric sarcomas. PMID: 26945340
    4. For metastatic YWHAE-rearranged HG-ESS, prolonged disease control following diagnosis was seen, with notable responses to anthracycline-based therapy. This emphasizes the need for appropriate molecular testing of uterine mesenchymal malignancies. PMID: 28390819
    5. phosphorus NMR and time-resolved tryptophan fluorescence measurements suggest that 14-3-3zeta interacts with the kinase domain of ASK1 in close proximity to its active site, thus indicating this interaction might block its accessibility and/or affect its conformation. PMID: 27514745
    6. The 14-3-3 family is dysregulated in schizophrenia, perhaps owing to specific regulatory mechanisms; the expression of the 14-3-3 epsilon, theta and zeta isoforms could be useful indicators of disease severity. PMID: 27030512
    7. Studies show that patients with clear cell sarcoma of the kidney (CCSK) and the fusion YWHAE-NUTM2B/E were relatively young, had low tumor volumes, and did not present with stage I disease which fail to identify an explicit clinical phenotype. PMID: 26542179
    8. Data show that the NS3 protein of dengue virus bound to 14-3-3 epsilon protein (14-3-3varepsilon) and prevented translocation of retinoic acid-inducible gene-I protein (RIG-I) to the adaptor MAVS protein and thereby blocked antiviral signaling. PMID: 26998762
    9. Report mutually exclusive BCOR internal tandem duplications and YWHAE-NUTM2 fusions in clear cell sarcoma of kidney. PMID: 27000436
    10. CEP131 is the key regulatory target of MK2 and 14-3-3 in centriolar satellite remodeling. PMID: 26616734
    11. 14-3-3epsilon might directly bind to CD13, which transmits its signal in chondrocytes to induce a catabolic phenotype similar to that observed in osteoarthritis. PMID: 26208633
    12. DAPK2-induced apoptosis is negatively regulated by Akt and 14-3-3 proteins. PMID: 26047703
    13. These data demonstrate a novel role for HDAC6 in controlling 14-3-3zeta binding activity. PMID: 25770209
    14. YWHAE-FAM22 rearrangement is associated with endometrial stromal sarcomas. PMID: 25244606
    15. Results suggest a specific role for YWHAE single nucleotide polymorphisms in the pathophysiology of schizophrenia. PMID: 25105667
    16. Cleft lip/palate has been found associated with 17p13.3 duplication involving a single candidate gene (YWHAE) in a father and his son. PMID: 23901910
    17. Data show that two isoforms of the 14-3-3 family, 14-3-3epsilon and 14-3-3 gamma, can stabilize Cdt2 independent of each other when overexpressed. PMID: 25154416
    18. These results suggest a possible role for the YWHAE genotype in the early development of the OFC sulcogyral pattern in patients with schizophrenia. PMID: 24561237
    19. data indicate that 14-3-3 is required for Bim expression and that it has an anti-cancer effect under pERK1/2 inhibition in 143B cells PMID: 24536031
    20. for endometrial sarcoma cases with high-grade morphology we recommend to test for YWHAE rearrangements by FISH break-apart PMID: 23599159
    21. YWHAE polymorphisms may be a genetic risk factor for HIV-associated neurocognitive disorder (HAND). PMID: 23982958
    22. Data indicate that 14-3-3 zeta, gamma, epsilon, and tau isoforms but not the sigma protein hydrolyze ATP. PMID: 24269678
    23. Carriers of the YWHAE*C allele of rs3752826 polymorphism have increased risk of suicidal behavior. PMID: 24450199
    24. findings suggest that FAK expression is correlated with and upregulated by 14-3-3epsilon via activation of NFkappaB PMID: 22934705
    25. interaction between NAV2 and 14-3-3epsilon PMID: 24161943
    26. 14-3-3epsilon associates with TAK1 in a phosphorylation-dependent manner to determine the cell fate of Bleomycin-treated HCC cells PMID: 23472066
    27. YWHAE gene duplication is associated with developmental, behavioral and brain abnormalities. PMID: 23813913
    28. 14-3-3epsilon may act as an important regulator in modulating tumor metastasis by promoting epithelial-mesenchymal transition as well as cell migration. PMID: 23483955
    29. The subcellular localization of full-length human MLF1 is 14-3-3epsilon-independent. PMID: 23271436
    30. The ERK/MAPK pathway is involved in the pathogenesis of gastric cancer; RKIP and 14-3-3epsilon exert an opposite effect on this pathway and the cells possibly via both direct and indirect reactions with the elements in this pathway. PMID: 22899242
    31. the -458G>T YWHAE variant contributes to the abnormal myocardial morphogenesis characteristic of LVNC as well as abnormal brain development, and implicate YWHAE as a novel candidate gene in pediatric cardiomyopathies. PMID: 23266643
    32. YWHAE gene contribute to the phenotype of small 17p13.3 chromosomal duplication in Miller-Dieker syndrome PMID: 23035971
    33. results suggest that YWHAE does play a major role in bipolar disorder in the Han Chinese population. PMID: 23140658
    34. These results showed that IgE is produced during malaria, and that the negative correlation between levels of IgE to 14-3-3 epsilon protein and parasitemia in asymptomatic malaria supports a role for these IgE molecules in defense mechanisms. PMID: 22984113
    35. Data obtained, which have never previously been reported, were used to modify the published 14-3-3 crystal structure using molecular modeling. PMID: 22580067
    36. When evaluating high-grade uterine sarcomas, cyclin D1 can be included in the immuno-histochemical panel as an indicator of YWHAE-FAM22endometrial stromal sarcoma. PMID: 22982899
    37. results indicate that the dosage effect of YWHAE varies from severe to very mild structural brain abnormalities, and suggest that the expression of YWHAE is associated with a complex mechanism of neuronal development PMID: 22887762
    38. 14-3-3epsilon is essential for the stable interaction of RIG-I with TRIM25, which facilitates RIG-I ubiquitination and initiation of innate immunity against hepatitis C virus and other pathogenic RNA viruses. PMID: 22607805
    39. Tumors with YWHAE-FAM22 rearrangements constitute a distinct group of endometrial stromal sarcoma (ESS), which is associated with high-grade morphology and aggressive clinical behavior compared to JAZF1 ESS. PMID: 22456610
    40. Collectively, these results identify a novel locus of apoptosomal regulation wherein MAPK signalling promotes Rsk-catalysed Apaf-1 phosphorylation and consequent binding of 14-3-3varepsilon, resulting in decreased cellular responsiveness to cytochrome c. PMID: 22246185
    41. Data present the high-resolution crystal structure of this binding motif [MLF1(29-42)pSer34] in complex with 14-3-3epsilon and analyse the interaction with isothermal titration calorimetry. PMID: 22151054
    42. Fluorescence in situ hybridization analysis demonstrated absolute specificity of YWHAE-FAM22A/B genetic rearrangement for high-grade endometrial stromal sarcoma. PMID: 22223660
    43. YWHAE does not play a major role in schizophrenia, major depressive disorder or bipolar disorder in the Han Chinese populatio PMID: 21184166
    44. Data suggest a regulatory circuitry involving Smad4 dependent up-regulation of KRT23 (directly or indirectly) which in turn modulates the interaction between KRT23 and 14-3-3epsilon leading to a cytoplasmic sequestration of 14-3-3epsilon. PMID: 21492476
    45. Results suggest that ERK and p38 MAPK regulate subcellular localization of Prdx6 by activation of 14-3-3epsilon as a chaperone protein, resulting in its translocation to acidic organelles. PMID: 21346153
    46. Overexpression of 14-3-3epsilon predicts tumour metastasis and poor survival in hepatocellular carcinoma PMID: 21401702
    47. For the first time, we found that TNF-alpha stimulation enhances the interactions between 14-3-3 epsilon and some key components in the mitogen-activated protein kinase (MAPK) signal module which is located at the immediate upstream of NF-kappaB. PMID: 20462248
    48. The 14-3-3 epsilon, zeta and theta may be involved in tumorigenesis of meningioma and be efficient markers for predicting the degree of malignancy in meningioma. PMID: 20388496
    49. Decreased expression of 14-3-3epsilon in contributes to the initiation and progression of laryngeal carcinoma by affecting apoptosis and invasion. PMID: 20565895
    50. the identification of a novel splicing variant of human 14-3-3 epsilon (14-3-3 epsilon sv), derived from a novel exon 1' insertion is reported. PMID: 20417184

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  • 亞細(xì)胞定位:
    Nucleus. Cytoplasm. Melanosome.
  • 蛋白家族:
    14-3-3 family
  • 數(shù)據(jù)庫鏈接:

    HGNC: 12851

    OMIM: 605066

    KEGG: hsa:7531

    STRING: 9606.ENSP00000264335

    UniGene: Hs.513851



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