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  4. VPS28 Recombinant Monoclonal Antibody

VPS28 Recombinant Monoclonal Antibody

  • 中文名稱：
    VPS28 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA445470A0HU
  • 規格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA445470A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela cell with CSB-RA445470A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA445470A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    Vacuolar protein sorting-associated protein 28 homolog (H-Vps28) (ESCRT-I complex subunit VPS28), VPS28
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide from human VPS28 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    12E3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
  • 基因功能參考文獻：
    1. CIIA attenuates neurotoxicity caused by OGD/R through inhibiting ASK1-dependent signaling events. PMID: 25098452
    2. USP8, and the endosomal sorting protein, VPS28, are the negative regulators of NOD2-induced IL-8 secretion. PMID: 25170077
    3. CIIA functions as a negative modulator of the SOS1-Ras signaling events initiated by peptide growth factors including EGF. PMID: 24522193
    4. EIAV Gag interacts with a cellular component of the VPS machinery, VPS28, to accomplish efficient particle release PMID: 12857913
  • 亞細胞定位：
    Cell membrane. Late endosome membrane; Peripheral membrane protein.
  • 蛋白家族：
    VPS28 family
  • 數據庫鏈接：

    HGNC: 18178

    OMIM: 611952

    KEGG: hsa:51160

    STRING: 9606.ENSP00000366565

    UniGene: Hs.418175



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