在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. Home
  2. 抗體
  3. 重組抗體
  4. UFC1 Recombinant Monoclonal Antibody

UFC1 Recombinant Monoclonal Antibody

  • 中文名稱：
    UFC1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA991252A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HEK293 whole cell lysate(30μg), COLO-205 whole cell lysate(30μg)
      All lanes: UFC1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 19 kDa
      Observed band size: 19 kDa
      Exposure time：3min
    • IHC image of CSB-RA991252A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA991252A0HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing MCF-7 cells stained with CSB-RA991252A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    UFC1
  • 別名：
    Ubiquitin-fold modifier-conjugating enzyme 1 (Ufm1-conjugating enzyme 1), UFC1
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide from human UFC1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    12C11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    E1-like enzyme which specifically catalyzes the second step in ufmylation. Accepts the ubiquitin-like modifier UFM1 from the E1 enzyme UBA5 and forms an intermediate with UFM1 via a thioester linkage. Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress.
  • 基因功能參考文獻(xiàn)：
    1. that UFC1 regulates survival of Osteoarthritis chondrocytes through physically association with miR-34a PMID: 27529373
    2. Autoantibodies binding to Ufc1 were found in 19 of 29 patients (66%) with active mycobacterial infections. PMID: 25213431
    3. Uba5 residues 364-404 were demonstrated to be necessary for the transthiolation of Ufm1 to Ufc1, and Uba5 381-404 was identified to be the minimal region for Ufc1 recognition PMID: 25084390
    4. UFC1 interact with the mRNA stabilizing protein HuR to increase levels of beta-catenin in hepatocellular carcinoma. PMID: 25449213
    5. NCAM140 interacts with ufc1 and its trafficking and endocytosis is upregulated in the presense of Ufm1. PMID: 24726913
    6. The structure of human Ufc1 was solved by using both NMR spectroscopy and X-ray crystallography PMID: 19101823
    7. Identification of a novel enzyme called UFC1 for Ufm1-conjugating enzyme 1. PMID: 15071506

    顯示更多

    收起更多

  • 蛋白家族：
    Ubiquitin-conjugating enzyme family, UFC1 subfamily
  • 數(shù)據(jù)庫鏈接：

    HGNC: 26941

    OMIM: 610554

    KEGG: hsa:51506

    STRING: 9606.ENSP00000356982

    UniGene: Hs.301412



主站蜘蛛池模板： 精品国产午夜肉伦伦影院| 国产在线视频www色| 牲交欧美兽交欧美| 婷婷五月小说| 中日韩高清无专码区2021| 久久亚洲中文字幕精品一区 | 伊人色综合久久天天| 亚洲熟妇久久国产精品| 国产肉丝袜视频在线观看| 亚洲日韩一区精品射精| 国产精品嫩草影院av| 久久伊人精品波多野结衣| 久久精品成人免费观看97| 国产青榴视频在线观看| 久久久久久久久久久久| 国产99久久久久久免费看| 日本道精品一区二区三区| 青青久久成人免费影院| 成人国产精品一区二区免费看| 无码乱码av天堂一区二区| 久久免费的精品国产v∧| 国产综合精品久久丫| 在线精品国精品国产尤物| 97久久人人超碰国产精品| 一区二区在线 | 欧洲| 伊人蕉久中文字幕无码专区 | 中国女人内谢69xxxx视频| 亚洲不卡av不卡一区二区| 亚洲国产成人久久久网站 | 国产精品人妻久久毛片高清无卡| 久久中文精品无码中文字幕下载| 色噜噜一区二区三区| 狠狠人妻久久久久久综合| 色老99久久九九爱精品| 国产亚洲精品自在久久vr| 少妇高潮喷水久久久久久久久久| 日本熟妇大乳| 99r在线精品视频在线播放| 麻豆果冻传媒2021精品传媒一区 | 久久97久久97精品免视看| 亚 洲 视 频 高 清 无 码|