在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. Home
  2. 抗體
  3. 重組抗體
  4. UBE2C Recombinant Monoclonal Antibody

UBE2C Recombinant Monoclonal Antibody

  • 中文名稱：
    UBE2C重組抗體
  • 貨號：
    CSB-RA025440MA1HU
  • 規格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA025440MA1HU diluted at 1:300 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA025440MA1HU diluted at 1:300 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing Hela cells stained with CSB-RA025440MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • 產品描述：
    UBE2C重組單克隆抗體（CSB-RA025440MA1HU）靶向泛素結合酶E2C（UBE2C），該蛋白作為泛素-蛋白酶體系統的核心成員，通過介導底物蛋白的泛素化修飾參與細胞周期調控及有絲分裂進程，尤其在腫瘤發生發展中顯示出異常表達特征。本產品經ELISA、免疫組化（IHC）和流式細胞術（FC）等多平臺嚴格驗證，在IHC應用中可清晰定位UBE2C在細胞核及胞質中的表達模式，推薦工作濃度為1:20-1:200；流式檢測中同樣表現出高靈敏度和特異性，適用于細胞表面及胞內抗原的定量分析。該抗體適用于探索UBE2C在腫瘤細胞增殖、DNA損傷修復及化療耐藥性中的分子機制，為癌癥生物學研究、細胞周期動態示蹤以及泛素化信號通路分析提供可靠工具，尤其適用于人源組織樣本或細胞系的相關實驗。產品經重組表達技術制備，具備批次間一致性高、交叉反應性低等優勢，可滿足高通量篩選及精準檢測需求，適用于基礎科研領域的蛋白質功能研究與分子互作分析。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Cyclin selective ubiquitin carrier protein antibody; dJ447F3.2 antibody; Mitotic specific ubiquitin conjugating enzyme antibody; UB E2C antibody; UBCH 10 antibody; UbcH10 antibody; UBE 2C antibody; Ube2c antibody; UBE2C_HUMAN antibody; Ubiquitin carrier protein C antibody; Ubiquitin carrier protein E2 C antibody; Ubiquitin carrier protein E2C antibody; Ubiquitin conjugating enzyme E2 C antibody; Ubiquitin conjugating enzyme E2C antibody; Ubiquitin protein ligase C antibody; Ubiquitin-conjugating enzyme E2 C antibody; Ubiquitin-protein ligase C antibody
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human UBE2C protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Mouse IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    22E1
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:20-1:200
    FC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
  • 基因功能參考文獻：
    1. UBE2C is involved in the anti-proliferative and pro-apoptotic functions of ECRG4 in esophageal squamous cell carcinoma. PMID: 29268240
    2. loss of BRCA1 function results in an increase in UBE2C expression and chemical resistance to doxorubicin in breast cancer cells. PMID: 28881292
    3. FoxM1 promotes glioma progression by enhancing UBE2C transcription PMID: 28767320
    4. SAG/RBX2 E3 ligase complexes with UBCH10 and UBE2S ubiquitin-conjugating enzymes to ubiquitylate beta-TrCP1 via K11-linkage for degradation. PMID: 27910872
    5. UBE2C and HOXA1 RNA and protein are differentially expressed in conventional and Spitz nevi and melanoma. PMID: 28657121
    6. High UBE2C expression is associated with esophageal squamous cell carcinoma. PMID: 27588470
    7. Urinary UBE2C cell-free RNA may be a valuable diagnostic marker for bladder cancer. PMID: 27528424
    8. A role was identified for UBE2C as a marker of the androgen signaling pathway in prostate cancer. PMID: 27550197
    9. Data suggest that the activity of AURKA might be regulated by UBE2C through regulating the activity of anaphasepromoting complex. UBE2C may be a new marker in the diagnosis of gastric cancer and may be a potential therapeutic target for the treatment of gastric adenocarcinoma. PMID: 28260026
    10. High UBCH10 expression is associated with bortezomib-resistance in multiple myeloma. PMID: 28000886
    11. the expression of UbcH10 in colorectal cancer samples as compared with healthy tissue sample from the same patient according to age at surgery, was examined. PMID: 26744065
    12. UbcH10 may promote gastric cancer growth. PMID: 27349176
    13. Identification of UBE2C as a target of wild-type and GOF mutant p53 further highlights the contribution of p53 in regulation of spindle assembly checkpoint PMID: 27129209
    14. The anaphase-promoting complex/cyclosome C activity in human cells is tuned by the combinatorial use of three E2 ubiquitin-conjugating enzymes, namely UBE2C, UBE2S, and UBE2D. PMID: 26904940
    15. Study shows an overexpression of UbcH10 mRNA and protein in the vast majority of colorectal cancer (CRC) patients analyzed and indicates that UbcH10 expression regulates CRC growth. PMID: 25917796
    16. the present study showed that miR-196a promoted cell proliferation by targeting UBE2C in breast cancer. Thus, miR-196a may be a potential oncogene in breast cancer and a promising therapeutic target in breast cancer treatment. PMID: 26062455
    17. UBE2C could regulate phospho-ERK1/2 level. PMID: 25832867
    18. Data suggest that ubiquitin-conjugating enzyme UbcH10 may be a promising target for the therapy of breast cancer. PMID: 25739083
    19. Upregulation of miR-17/20a promotes gastric cancer cell growth by targeting UBE2C. PMID: 25760688
    20. UbcH10 overexpression increases carcinogenesis and blocks ALLN susceptibility in colorectal cancer. PMID: 25376843
    21. in addition to binding APC11's RING, UBCH10 is corecruited via interactions with APC2 PMID: 25825779
    22. excess E2F1 due to Rb inactivation recruits the complex of Cdc20 and the anaphase-promoting complex/cyclosome to deregulate the expression of UBCH10 PMID: 25368385
    23. UBE2C may be a marker for diagnosis of nonpalpable breast lesions but not benign or malignant tumors in mammography core biopsies. Suppression of UBE2C may be a potential therapy target in breast cancer. PMID: 24699941
    24. the UBE2C gene is capable of promoting proliferation and invasion of lung cancer cells. Therefore, UBE2C may play a pivotal role in development and invasion of lung cancer. PMID: 24815438
    25. Results suggest that UBE2C acts as an oncogene in prostate cancer progression. PMID: 24512726
    26. Data suggest that ubiquitin conjugating enzyme UBE2C may serve as a potential therapeutic target aimed at inducing radiation and chemo sensitization. PMID: 24072565
    27. Elevated levels of S100A8 and UBE2C were observed. PMID: 24114735
    28. UBE2C positivity is independently associated with shorter cancer-specific survival in bladder cancer patients who underwent radical cystectomy. PMID: 23826418
    29. Data indicate that expression-based risk indices of three genes UBE2C, TPX2, and MELK were more strongly associated with poor 5-year survival in adenocarcinoma patients. PMID: 23357462
    30. High expression of UbcH10 was associated with pancreatic ductal adenocarcinoma. PMID: 23355337
    31. the expression of cyclin D1, MCM7, TRIM29, and UBE2C was found to be significantly associated with progression to muscle-invasive bladder cancer. PMID: 23201130
    32. UbcH10 overexpression has a critical role in lung carcinogenesis, and the evaluation of UbcH10 expression levels may be a new tool for the characterisation of non small cell lung carcinoma. PMID: 23102841
    33. Data sugggest that UbcH10 may play an important role in non-small cell lung cancer (NSCLC) carcinogenesis, and silencing UbcH10 may represent a potential therapeutic strategy for NSCLC. PMID: 22760214
    34. Overexpression of UbcH10 may be a useful indicator of endometrial carcinoma. PMID: 22415060
    35. growth rate of SiHa and HeLa transfected with dominant negative-UBE2C was significantly reduced compared to vector control PMID: 22694363
    36. High UBE2C mRNA expression was found to be of adverse prognostic significance in high-risk breast cancer patients PMID: 22056852
    37. UbcH10 expression was associated with tumor invasion of the portal vein, tumor size, TNM staging, and tumor differentiation in hepatocellular carcinoma. PMID: 21354912
    38. Effect of UBE2C overexpression on the proliferation of 293T cell line PMID: 21651863
    39. Mediator complex subunit (MED)1 phosphorylation leads to UBE2C locus looping, UBE2C gene expression and cell growth in castration-resistant prostate cancer. PMID: 21556051
    40. Spindle assembly checkpoint protein Cdc20 transcriptionally activates expression of ubiquitin carrier protein UbcH10. PMID: 21454660
    41. Elevated expression of UbcH10 is associated with breast cancer. PMID: 19038004
    42. elevated levels of Ubch10 and Cdc20 degrade cyclin B in hpv-16 iinfected cells, required for exit from mitosis, permitting initiation of the next round of DNA synthesis and cell cycle progression. PMID: 20739533
    43. The unstructured N-terminal region of UbcH10 directly binds the 19 S regulatory complex of the 26 S proteasome, and it mediates the initiation of substrate translocation. PMID: 20007692
    44. The overexpression of UbcH10 gene plays a critical role in the carcinogenesis and tumor progression of colorectal cancer. PMID: 19779934
    45. show that UbcH10 overexpression leads to precocious degradation of cyclin B by the anaphase-promoting complex/cyclosome, supernumerary centrioles, lagging chromosomes, and aneuploidy. PMID: 20065091
    46. Data imply that knocking-down UbcH10 protein expression may represent a potential therapeutic option for glioma. PMID: 19657671
    47. UbcH10 is highly expressed in various human primary tumors and UbcH10 has an ability to promote cell growth and malignant transformation. PMID: 12874022
    48. UbcH10 plays an important role in tumor development and that its inhibition in combination with agonists of the TRAIL receptor may provide an enhanced therapeutic index PMID: 15208666
    49. UbcH10 overexpression is involved in thyroid cell proliferation and may represent a marker of thyroid anaplastic carcinomas PMID: 16106252
    50. results showing aberrations in levels of gene expression and locus copy number of ubiquitin-conjugating enzyme E2C gene (UBE2C) suggest that this gene may play an important role in tumor progression leading to advanced colon cancer with liver metastasis PMID: 16772118

    顯示更多

    收起更多

  • 蛋白家族：
    Ubiquitin-conjugating enzyme family
  • 數據庫鏈接：

    HGNC: 15937

    OMIM: 605574

    KEGG: hsa:11065

    STRING: 9606.ENSP00000348838

    UniGene: Hs.93002



主站蜘蛛池模板： 人与动人物xxxx毛片人与狍| 无码国产精品一区二区免费模式| 苍井空张开腿实干12次| 亚洲欧美日韩久久精品| 欧美肥老太牲交| 日本japanese漂亮丰满| 成人精品一区日本无码网| 国产成人精品综合久久久久| 人妻avav中文系列久久| 亚洲成av人片在线观l看福利1| 自慰无码一区二区三区| 久久99精品国产麻豆婷婷| 成年轻人电影免费无码| 国产精品自在在线午夜免费| 同性男男黄g片免费网站| 日本少妇高潮喷水视频| 亚洲精品宾馆在线精品酒店| 亚洲人精品亚洲人成在线| 国产精品美女久久久久久久久| 柠檬福利精品视频导航| 午夜伦4480yy私人影院久久| av制服丝袜白丝国产网站| 国产亚洲精品久久久久久无几年桃 | 日本两人免费观看的视频| 中文字幕人妻第一区| 亚洲色成人www永久在线观看| 亚洲不卡中文字幕无码| av天堂永久资源网亚洲高清| 久久热最新网站获取| 天天爽夜夜爽人人爽| 亚洲欧美综合区丁香五月小说| 偷窥 亚洲 另类 图片 熟女| 欧美潮喷少妇100| 脱了美女内裤猛烈进入gif| 欧美大片18禁aaa免费视频| 亚洲乱码中文字幕小综合| 国产精品一久久香蕉国产线看观看| 无码专区天天躁天天躁在线| 国产精品成人观看视频| 亚洲成av人的天堂在线观看| 色婷婷久久久swag精品|