TOMM20 Recombinant Monoclonal Antibody
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中文名稱：TOMM20 Recombinant Monoclonal Antibody
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貨號(hào)：CSB-RA092384A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: K562 whole cell lysate(30μg), THP-1 whole cell lysate(30μg), SH-SY5Y whole cell lysate(30μg), U-251MG whole cell lysate(30μg), Jurkat whole cell lysate(30μg), HEK293 whole cell lysate(30μg), A431 whole cell lysate(30μg), HeLa whole cell lysate(30μg), PC-3 whole cell lysate(30μg)
All lanes: TOMM20 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time：1min -
IHC image of CSB-RA092384A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Immunofluorescence staining of U-251MG cell with CSB-RA092384A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA092384A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產(chǎn)品詳情
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Uniprot No.：
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基因名：
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別名：Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial 20 kDa outer membrane protein) (Outer mitochondrial membrane receptor Tom20), TOMM20, KIAA0016
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反應(yīng)種屬：Human
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免疫原：A synthesized peptide from human TOMM20 protein
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號(hào)：15F9
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
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基因功能參考文獻(xiàn)：
- TOMM20 and MCT1 were highly expressed in diffuse large B-cell lymphoma lymphocytes, indicating an OXPHOS phenotype, whereas non-neoplastic lymphocytes in the control samples did not express these markers. PMID: 29248132
- TOMM20, MCT1, and MCT4 expression was significantly different in Hodgkin and Reed Sternberg (HRS) cells. HRS have high expression of TOMM20, while tumor associated macrophages have absent TOMM20 expression. Tumor-infiltrating lymphocytes have low TOMM20 expression. Reactive lymph nodes in contrast to cHL tumors had low TOMM20, MCT1, and MCT4 expression in lymphocytes and macrophages. PMID: 29248133
- Gsk3beta and Tomm20 are substrates of the SCFFbxo7/PARK15 ubiquitin ligase associated with Parkinson's disease PMID: 27503909
- TOMM20 protein is highly expressed in both anaplastic thyroid cancer and papillary thyroid cancer compared to non-cancerous thyroid tissue. PMID: 26615136
- Tom20 was ubiquitously distributed in the organ of Corti, allowing well-delineated visualization of inner and outer hair cells. In the inner ear of aged subjects with Meniere's disease, there was decreased expression of Tom20. PMID: 23165776
- a novel model in which Tom20 binds Tom70 to facilitate preprotein release from the chaperones by competition PMID: 21771790
- Tom34 unlike Tom20 does not interact with the leader sequences of mitochondrial precursor proteins PMID: 11913975
- the cytosolic domains of Tom20 and Tom22 function to maintain their substrate preproteins unfolded and prevent them from aggregating on the mitochondrial surface PMID: 14699115
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亞細(xì)胞定位：Mitochondrion outer membrane; Single-pass membrane protein.
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蛋白家族：Tom20 family
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數(shù)據(jù)庫鏈接：
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