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SEC61A1/SEC61A2 Recombinant Monoclonal Antibody

  • 中文名稱:
    SEC61A1/SEC61A2 Recombinant Monoclonal Antibody
  • 貨號:
    CSB-RA293494A0HU
  • 規格:
    ¥1320
  • 圖片:
    • Western Blot
      Positive WB detected in: U-251MG whole cell lysate(30μg),SH-SY5Y whole cell lysate(30μg),A431 whole cell lysate(30μg),HepG2 whole cell lysate(30μg),Hela whole cell lysate(30μg),A549 whole cell lysate(30μg), Mouse liver tissue lysate(30μg), Rat liver tissue lysate(30μg)
      All lanes: SEC61A1/2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 52 kDa
      Observed band size: 52 kDa
      Exposure time:1min
    • IHC image of CSB-RA293494A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela cell with CSB-RA293494A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA293494A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他:

產品詳情

  • Uniprot No.:
  • 基因名:
    SEC61A1/SEC61A2
  • 別名:
    Protein transport protein Sec61 subunit alpha isoform 1 (Sec61 alpha-1), SEC61A1, SEC61A, Protein transport protein Sec61 subunit alpha isoform 2 (Sec61 alpha-2), SEC61A2
  • 反應種屬:
    Human, Mouse, Rat
  • 免疫原:
    A synthesized peptide derived from human SEC61A1/SEC61A2
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    Rabbit IgG
  • 純化方式:
    Affinity-chromatography
  • 克隆號:
    19C7
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價



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