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  4. RCC1 Recombinant Monoclonal Antibody

RCC1 Recombinant Monoclonal Antibody

  • 中文名稱：
    RCC1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA589725A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HeLa whole cell lysate(30μg), HepG2 whole cell lysate(30μg), Jurkat whole cell lysate(30μg), HEK293 whole cell lysate(30μg), SW620 whole cell lysate(30μg), HT-29 whole cell lysate(30μg), K562 whole cell lysate(30μg), A549 whole cell lysate(30μg)
      All lanes: RCC1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 45 kDa
      Observed band size: 50 kDa
      Exposure time: 1min
    • IHC image of CSB-RA589725A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA589725A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing jurkat cells stained with CSB-RA589725A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    RCC1
  • 別名：
    Cell cycle regulatory protein antibody; CHC 1 antibody; CHC1 antibody; Chromosome condensation 1 antibody; Chromosome condensation protein 1 antibody; Guanine nucleotide releasing protein antibody; HERC2 antibody; Ran GEF antibody; RanGEF antibody; RCC 1 antibody; RCC1 antibody; RCC1 I antibody; RCC1_HUMAN antibody; Regulator of chromosome condensation 1 antibody; Regulator of chromosome condensation antibody; SHEP1 antibody; SNHG3 RCC1 antibody; SNHG3 RCC1 readthrough transcript antibody
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human RCC1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    2A4
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis. Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis. Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA.
  • 基因功能參考文獻：
    1. This study provides first evidence for a mutation in RCC1 as a breast cancer susceptibility allele PMID: 29363114
    2. Importin alpha3 facilitates the nuclear transport of the Ran guanine nucleotide exchange factor RCC1. PMID: 29042532
    3. this study has demonstrated that EBNA1 and RCC1 closely associate on the chromosomes during metaphase, suggesting an essential role for the interaction during this phase, perhaps in tethering EBNA1 to mitotic chromosomes. PMID: 28284242
    4. RCC1 expression enables the proliferation of cells that sustain DNA damage. RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage-induced cell senescence. PMID: 26864624
    5. RCC1 expression is frequently lost in poorly differentiated gastric cell lines and gastric carcinoma tissues. Loss of RCC1 expression is correlated with tumor differentiation and depth of invasion. PMID: 26823742
    6. stress can disrupt the Ran gradients through RCC1-dependent and RCC1-independent mechanisms PMID: 25452301
    7. PI3Kbeta regulates the nuclear envelope through upstream regulation of RCC1 and Ran PMID: 25348717
    8. RCC1 facilitates a tight binding between the encephalomyocarditis virus leader and cellular Ran GTPase. PMID: 23536659
    9. RCC1 can induce bipolar spindle formation. Remarkably, RCC1 beads oscillate within spindles from pole to pole, a behavior that could be converted to a more typical, stable association by the addition of a kinesin together with RCC1. PMID: 22215983
    10. discovery of the first alpha-N-methyltransferase, which we named N-terminal RCC1 methyltransferase (NRMT). PMID: 20668449
    11. The authors find that RCC1 uses a conformationally flexible loop region we have termed the switchback loop in addition to its N-terminal tail to bind to the nucleosome. PMID: 20347844
    12. RanBP3 associates with the Ran-specific guanine nucleotide exchange factor, regulator of chromosome condensation 1 (RCC1). PMID: 11932251
    13. These results indicate that the generation of Ran-GTP in the vicinity of chromosomes by RCC1 is important for the fidelity of mitotic spindle assembly in human cells. PMID: 12194828
    14. RCC1 release from chromatin in vitro requires a factor(s) distinct from, or in addition to, Ran PMID: 14565978
    15. phosphorylation of the NLS of RCC1 is required to prevent the binding of importin alpha and beta to RCC1, thereby allowing RCC1 to couple RanGTP production to chromosome binding PMID: 15014043
    16. This 30 kb deletion contains the exons coding for the RCC1-like domain of RPGR. It is the first report of a macrodeletion that spans the entire RCC1-like domain of RPGR in X-linked retinitis pigmentosa patients PMID: 16052169
    17. HSV-1 replication is blocked in cell line tsBN2 encoding a temperature sensitive RCC1 protein. PMID: 17251553
    18. Propose bimodal attachment mechanism for RCC1 in which the tail promotes stable RCC1 association with chromatin through DNA binding in an alpha-N-methylation-dependent manner. PMID: 17435751
    19. These results show that human RCC1 isoforms have distinct chromatin binding properties, different molecular interactions, and are selectively regulated by phosphorylation, as determined by their different NTRs. PMID: 17855385
    20. chromosome 1p36 was established to have linkage for adhd. PMID: 18439570
    21. A review of the function and structure of RCC1 and RCC1 superfamily genes and proteins. PMID: 18442486
    22. reduced binding affinity of RCC1 to the mitotic chromosome, which led to a reduction of RanGTP level. PMID: 18712773
    23. The N-terminal tail of RCC1 is essential for association with DNA but inhibits histone binding. PMID: 18762580
    24. Therefore, we propose that RCC1 reads the histone code created by caspase-activated Mst1 to initiate apoptosis by reducing the level of RanGTP in the nucleus. PMID: 19060893

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  • 亞細胞定位：
    Nucleus. Chromosome. Cytoplasm.
  • 數據庫鏈接：

    HGNC: 1913

    OMIM: 179710

    KEGG: hsa:1104

    UniGene: Hs.469723



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