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  4. RBM8A Recombinant Monoclonal Antibody

RBM8A Recombinant Monoclonal Antibody

  • 中文名稱：
    RBM8A Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA171029A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: MCF-7 whole cell lysate(30μg),K562 whole cell lysate(30μg),Jurkat whole cell lysate(30μg),Hela whole cell lysate(30μg), Rat brain tissue lysate(20μg)
      All lanes: RBM8A antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 20 kDa
      Observed band size: 20 kDa
      Exposure time：1min
    • Immunofluorescence staining of MCF-7 cell with CSB-RA171029A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing MCF-7 cells stained with CSB-RA171029A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    RBM8A
  • 別名：
    Binder of OVCA1 1 antibody; Binder of OVCA1-1 antibody; BOV 1 antibody; BOV 1A antibody; BOV 1B antibody; BOV 1C antibody; BOV-1 antibody; BOV1 antibody; BOV1A antibody; BOV1B antibody; BOV1C antibody; HSPC 114 antibody; HSPC114 antibody; MDS 014 antibody; MDS014 antibody; RBM 8 antibody; RBM 8A antibody; RBM 8B antibody; RBM8 antibody; rbm8a antibody; RBM8A_HUMAN antibody; RBM8B antibody; Ribonucleoprotein RBM 8 antibody; Ribonucleoprotein RBM 8A antibody; Ribonucleoprotein RBM8 antibody; Ribonucleoprotein RBM8A antibody; RNA binding motif protein 8 antibody; RNA binding motif protein 8A antibody; RNA binding motif protein 8B antibody; RNA binding protein 8A antibody; RNA binding protein Y14 antibody; RNA-binding motif protein 8A antibody; RNA-binding protein 8A antibody; RNA-binding protein Y14 antibody; ZNRP antibody; ZRNP 1 antibody; ZRNP1 antibody
  • 反應種屬：
    Human, Rat
  • 免疫原：
    A synthesized peptide derived from human RBM8A
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    7F9
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Required for pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.
  • 基因功能參考文獻：
    1. Findings demonstrate that Y14 nucleoplasmic localization is regulated not only by the previously reported N-terminal localization signal but also by the C-terminal serine/arginine repeat-containing region through phosphorylation and MAGOH binding to Y14. PMID: 29330450
    2. A direct link between Y14 and p53 expression and suggests a function for Y14 in DNA damage signaling. PMID: 28361991
    3. Results demonstrated that RBM8A is highly expressed in hepatocellular carcinoma (HCC), and patients with lower RBM8A expression level had longer overall survival and progression free survival. Also, results indicate that RBM8A promotes invasion and metastasis via epithelial-mesenchymal transition signaling pathway. PMID: 28259942
    4. Y14 may selectively and differentially modulate protein biosynthesis. PMID: 26887951
    5. this data suggests a novel role of RBM8a in the regulation of neurodevelopment. PMID: 26094033
    6. Mutations of RBM8A and TBX6 are associated with disorders of the mullerian ducts. PMID: 25813282
    7. It was demonstrated that Rbm8a is an essential neurogenesis regulator in embryonic cortical development. PMID: 25948253
    8. Depletion of RNA-binding protein RBM8A (Y14) causes cell cycle deficiency and apoptosis in human tumor cells. PMID: 23970407
    9. Y14 positively regulates signals for TNF-alpha-induced IL-6 production at multiple steps beyond an exon junction complex protein. PMID: 23817415
    10. Y14 mutants, which are deficient in binding to Magoh, could still be localized to the nucleus, suggesting the existence of both the nuclear import pathway and function for Y14 unaccompanied by Magoh PMID: 22355610
    11. Data show that Y14 interacts directly with the decapping factor Dcp2 and the 5' cap structure of mRNAs via different but overlapping domains. PMID: 23115303
    12. findings show that the two regulatory SNPs result in diminished RBM8A transcription in vitro and that Y14 expression is reduced in platelets from individuals with thrombocytopenia with absent radii (TAR) PMID: 22366785
    13. Y14 provides a regulatory link between pre-mRNA splicing and snRNP biogenesis. PMID: 21209085
    14. conserved physical linkage in medaka and human genomes PMID: 12054603
    15. Tethered function analysis shows that the Y14/hUpf3b interaction is essential for nonsense-mediated decay, while surprisingly the interaction between hUpf3b and hUpf2 is not. PMID: 12718880
    16. antagonistic post-translational modifications of Y14 may be involved in the remodeling of Y14-containing mRNPs PMID: 16100109
    17. The stable association of multiprotein exon junction complex core with RNA is maintained by inhibition of eIF4AIII ATPase activity by MAGOH-Y14. PMID: 16170325
    18. crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase eukaryotic initiation factor 4AIII bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic PMID: 16931718
    19. Increased TAP binding correlates with increased SF2/ASF binding, but not increased REF/Aly or Y14 binding. PMID: 18243119
    20. Y14 interacts with STAT3 and regulates the transcriptional activation of STAT3 by influencing the tyrosine-phosphorylation of STAT3. PMID: 18503751
    21. These results indicate that MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and Y14. PMID: 19254694

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  • 亞細胞定位：
    Nucleus. Nucleus speckle. Cytoplasm.
  • 蛋白家族：
    RBM8A family
  • 組織特異性：
    Ubiquitous.
  • 數據庫鏈接：

    HGNC: 9905

    OMIM: 605313

    KEGG: hsa:9939

    STRING: 9606.ENSP00000333001

    UniGene: Hs.591455



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