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  4. PSMA1 Recombinant Monoclonal Antibody

PSMA1 Recombinant Monoclonal Antibody

  • 中文名稱：
    PSMA1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA276081A0HU
  • 規格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA276081A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA276081A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of PC-3 cell with CSB-RA276081A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA276081A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    30 kDa prosomal protein antibody; HC 2 antibody; HC2 antibody; Macropain subunit C2 antibody; Macropain subunit nu antibody; MGC14542 antibody; MGC14575 antibody; MGC14751 antibody; MGC1667 antibody; MGC21459 antibody; MGC22853 antibody; MGC23915 antibody; Multicatalytic endopeptidase complex subunit C2 antibody; NU antibody; PROS 30 antibody; PROS-30 antibody; PROS30 antibody; Proteasome (prosome macropain) subunit alpha type 1 antibody; Proteasome alpha 1 subunit antibody; Proteasome component C2 antibody; Proteasome nu chain antibody; Proteasome subunit alpha type 1 antibody; Proteasome subunit alpha type I antibody; Proteasome subunit alpha type-1 antibody; Proteasome subunit nu antibody; Protein P30 33K antibody; PSA1_HUMAN antibody; PSC 2 antibody; PSC2 antibody; PSMA 1 antibody; psmA1 antibody
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human PSMA1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    16H4
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
  • 基因功能參考文獻：
    1. In thyroid hemiagenesis, genomic examinations revealed the presence of four recurrent defects (three deletions and one duplication) affecting highly conservative proteasome genes PSMA1, PSMA3, and PSMD3. PMID: 28390009
    2. Data indicate that RNA interference screens identified PSMA1, a component of the 20S proteasome as target of non-small cell lung cancer (NSCLC) cytotoxicity. PMID: 24040035
    3. LC/MS/MS analysis revealed that the C-terminal 16 residues of sperm alpha6 subunit are processed. PMID: 21703233
  • 亞細胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Peptidase T1A family
  • 數據庫鏈接：

    HGNC: 9530

    OMIM: 602854

    KEGG: hsa:5682

    STRING: 9606.ENSP00000414359

    UniGene: Hs.102798



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