在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. Home
  2. 抗體
  3. 重組抗體
  4. PRPF4 Recombinant Monoclonal Antibody

PRPF4 Recombinant Monoclonal Antibody

  • 中文名稱：
    PRPF4 Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA998693A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate(20μg),HEK293 whole cell lysate(20μg), Hela whole cell lysate(20μg), COLO-205 whole cell lysate(20μg),Raji whole cell lysate(20μg),HepG2 whole cell lysate(20μg)
      All lanes: PRPF4 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 58 kDa
      Observed band size: 58 kDa
      Exposure time：2min
    • IHC image of CSB-RA998693A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA998693A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing Hela cells stained with CSB-RA998693A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    PRPF4
  • 別名：
    hPrp4 antibody; HPRP4P antibody; PRP4 antibody; PRP4 homolog antibody; PRP4 pre mRNA processing factor 4 homolog (yeast) antibody; PRP4/STK/WD splicing factor antibody; PRP4_HUMAN antibody; Prp4p antibody; PRPF4 antibody; RP70 antibody; SNRNP60 antibody; U4/U6 small nuclear ribonucleoprotein Prp4 antibody; U4/U6 snRNP 60 kDa protein antibody; WD splicing factor Prp4 antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human PRPF4
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    15E6
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex).
  • 基因功能參考文獻(xiàn)：
    1. The p.R192H variant of PRPF4 represents a functional null allele which compromises the function of the tri-snRNP, reinforcing the notion that this spliceosomal particle is of crucial importance in the physiology of the retina. PMID: 25383878
    2. PRPF4 missense mutations is associated with autosomal dominant retinitis pigmentosa. PMID: 24419317
    3. the requirement of enzymatic activity of PRP4 in regulating cancer cell growth and identified an array of potential novel substrates through orthogonal proteomics approaches PMID: 24003220
    4. interaction between Hprp3p and Hprp4p PMID: 11971898
    5. PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores. PMID: 17998396
    6. PRP-4 belongs to the serine/threonine protein kinase family, plays a role in pre-mRNA splicing and cell mitosis, and interacts with CLK1 PMID: 18687998

    顯示更多

    收起更多

  • 相關(guān)疾病：
    Retinitis pigmentosa 70 (RP70)
  • 亞細(xì)胞定位：
    Nucleus. Nucleus speckle.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 17349

    OMIM: 607795

    KEGG: hsa:9128

    STRING: 9606.ENSP00000363313

    UniGene: Hs.744014



主站蜘蛛池模板： 丰满人妻翻云覆雨呻吟视频| 国产成人理论无码电影网| 少妇无码av无码专区| 97碰碰碰人妻无码视频| 久久综合亚洲色hezyo国产| 久青草无码视频在线观看| 欧美506070老妇乱子伦| 国产好吊看视频在线观看| 国产精品人成视频免费国产| 天天摸夜夜添狠狠添婷婷| 毛片免费视频肛交颜射免费视频| 人妻无码一区二区三区 tv| 国产精品色情国产三级在| 无码人妻丝袜视频在线播免费| 内射女校花一区二区三区| 久久无码人妻一区二区三区| 欧美色欧美亚洲另类二区| 国产人妻精品区一区二区三区| 无码抽搐高潮喷水流白浆| 久久亚洲精品成人av| 美女内射视频www网站午夜| 99久久无色码中文字幕人妻蜜柚| 人妻aⅴ中文字幕无码| 亚洲精品久久久久玩吗| 人妻中文字幕av无码专区 | 国产精品国语对白露脸在线播放 | 国产成人精品a视频| 五月丁香综合缴情六月小说| 日本一卡2卡3卡4卡5卡精品视频| 天堂а√在线地址8| 成在人线av无码免费| 在线亚洲高清揄拍自拍一品区| 亚洲午夜久久久无码精品网红a片| 蜜臀av无码精品人妻色欲| 少妇性l交大片7724com| 亚洲色成人四虎在线观看| 久久97精品久久久久久久不卡| 老熟妇仑乱视频一区二区| 无码h肉动漫在线观看免费 | 亚洲精品国产品国语原创| 国产精品麻豆成人av电影艾秋 |