在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. Home
  2. 抗體
  3. 重組抗體
  4. PPP2R5E Recombinant Monoclonal Antibody

PPP2R5E Recombinant Monoclonal Antibody

  • 中文名稱：
    PPP2R5E Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA834341A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: U-251MG whole cell lysate(30μg), HEK293 whole cell lysate(30μg), COLO-205 whole cell lysate(30μg), Hela whole cell lysate(30μg), PC-3 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg)
      All lanes: PPP2R5E polymerase beta antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 55 kDa
      Observed band size: 50 kDa
      Exposure time：3min
    • IHC image of CSB-RA834341A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA834341A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing Hela cells stained with CSB-RA834341A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform (PP2A B subunit isoform B'-epsilon) (PP2A B subunit isoform B56-epsilon) (PP2A B subunit isoform PR61-epsilon) (PP2A B subunit isoform R5-epsilon), PPP2R5E
  • 反應種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide from human PPP2R5E protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    5B11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • 基因功能參考文獻：
    1. results suggest that the PPP2R5E phosphatase may contribute to microtubule organization by stabilizing MTCL1. PMID: 27521566
    2. PP2A holoenzyme containing B56 is responsible for the dephosphorylation of gamma-H2AX and regulation of DNA repair of double strand breaks induced by camptothecin. PMID: 25772433
    3. the significance of miR-23a and PPP2R5E in the proliferation and apoptosis of gastric cancer cells PMID: 24997345
    4. Downregulation of PPP2R5E affects the oncogenic potential of leukemic cells. PMID: 23812941
    5. identified the phosphorylation of hSgo2 by Aurora B at the N-terminal coiled-coil region and the middle region, and showed that these phosphorylations separately promote binding of hSgo2 to PP2A and MCAK PMID: 20889715
    6. The PPP2R5E gene is identified as harboring genetic variants that can affect human cancer and are possibly under evolutionary selection pressure. PMID: 19773383

    顯示更多

    收起更多

  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Phosphatase 2A regulatory subunit B56 family
  • 數據庫鏈接：

    HGNC: 9313

    OMIM: 601647

    KEGG: hsa:5529

    STRING: 9606.ENSP00000337641

    UniGene: Hs.334868



主站蜘蛛池模板： 国产人妻人伦精品1国产丝袜| 巨乳美乳一区二区三区| 真人无码作爱免费视频| 国产成人无码视频一区二区三区| 青草伊人久久综在合线亚洲观看| 国产男女猛烈视频在线观看 | 中文毛片无遮挡高潮免费| 国产精品内射后入合集| 97无码免费人妻超级碰碰夜夜| 欧美日韩一本无线码专区| 亚洲欧美日韩一区在线观看 | 乱码午夜-极品国产内射| 国产女人高潮抽搐叫床视频| 日日噜噜夜夜狠狠va视频| 欧美老妇与zozoz0交| 欧美96在线 | 欧| 人妻丰满熟妇a无码区| 亚洲中文字幕无码乱线| 国产女爽爽精品视频天美传媒| 无码国产偷倩在线播放| 免费不卡无码av在线观看| 国产a∨精品一区二区三区不卡| 妺妺窝人体色www在线小说| 免费看美女部位隐私网站| 亚洲欧美日韩综合av| 国产精品久久久影视青草| 婷婷久久香蕉五月综合| 亚洲精品无码精品mv在线观看 | av无码中出一区二区三区| 亚洲精品一区二区三区四区乱码| 好吊妞无缓冲视频观看| 樱花草在线社区www日本影院| 国内老熟妇乱子伦视频 | 夜夜添无码试看一区二区三区 | 亚洲熟女一区二区三区| 国产一区二区三区免费高清在线播放| 在线无码av一区二区三区| 亚洲国产av美女网站| 久精品国产欧美亚洲色aⅴ大片| 国内精品无码一区二区三区| 综合久久给合久久狠狠狠97色 |