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  4. PPP2R5E Recombinant Monoclonal Antibody

PPP2R5E Recombinant Monoclonal Antibody

  • 中文名稱：
    PPP2R5E Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA834341A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: U-251MG whole cell lysate(30μg), HEK293 whole cell lysate(30μg), COLO-205 whole cell lysate(30μg), Hela whole cell lysate(30μg), PC-3 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg)
      All lanes: PPP2R5E polymerase beta antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 55 kDa
      Observed band size: 50 kDa
      Exposure time：3min
    • IHC image of CSB-RA834341A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA834341A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing Hela cells stained with CSB-RA834341A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform (PP2A B subunit isoform B'-epsilon) (PP2A B subunit isoform B56-epsilon) (PP2A B subunit isoform PR61-epsilon) (PP2A B subunit isoform R5-epsilon), PPP2R5E
  • 反應種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide from human PPP2R5E protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    5B11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • 基因功能參考文獻：
    1. results suggest that the PPP2R5E phosphatase may contribute to microtubule organization by stabilizing MTCL1. PMID: 27521566
    2. PP2A holoenzyme containing B56 is responsible for the dephosphorylation of gamma-H2AX and regulation of DNA repair of double strand breaks induced by camptothecin. PMID: 25772433
    3. the significance of miR-23a and PPP2R5E in the proliferation and apoptosis of gastric cancer cells PMID: 24997345
    4. Downregulation of PPP2R5E affects the oncogenic potential of leukemic cells. PMID: 23812941
    5. identified the phosphorylation of hSgo2 by Aurora B at the N-terminal coiled-coil region and the middle region, and showed that these phosphorylations separately promote binding of hSgo2 to PP2A and MCAK PMID: 20889715
    6. The PPP2R5E gene is identified as harboring genetic variants that can affect human cancer and are possibly under evolutionary selection pressure. PMID: 19773383

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Phosphatase 2A regulatory subunit B56 family
  • 數據庫鏈接：

    HGNC: 9313

    OMIM: 601647

    KEGG: hsa:5529

    STRING: 9606.ENSP00000337641

    UniGene: Hs.334868



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