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MAS1L Recombinant Monoclonal Antibody

  • 中文名稱:
    MAS1L重組抗體
  • 貨號:
    CSB-RA971033A0HU
  • 規格:
    ¥1320
  • 圖片:
    • Western Blot
      Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Jurkat whole cell lysate, Rat liver tissue
      All lanes: MAS1L antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 43 kDa
      Observed band size: 36-50 kDa
    • Immunofluorescence staining of MCF-7 cell with CSB-RA971033A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with CSB-RA971033A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-RA971033A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions.
  • 其他:

產品詳情

  • 產品描述:
    CSB-RA971033A0HU是靶向MAS1L蛋白的重組單克隆抗體,適用于多種實驗場景的科研需求。該抗體針對MAS1L受體特異性設計,可精確識別其天然構象表位,在多種驗證實驗中展現出優異的性能:Western Blot(WB)推薦稀釋比例1:500-1:2000,可清晰檢測目標蛋白條帶;免疫熒光(IF)和流式細胞術(FC)分別在1:50-1:200稀釋范圍內呈現高信噪比染色效果,適用于亞細胞定位及表面標志物分析。MAS1L作為G蛋白偶聯受體家族成員,參與調控細胞信號轉導和組織穩態,本產品可支持其在細胞功能研究(如增殖調控、分化機制探索)及疾病模型(如心血管或腫瘤相關基礎研究)中的表達分析。經ELISA驗證,該抗體具備高批次穩定性與低交叉反應性,可滿足復雜樣本的定量檢測需求。適用于體外培養細胞、組織裂解液等樣本類型,為受體功能研究、信號通路解析及分子互作實驗提供可靠工具。
  • Uniprot No.:
  • 基因名:
  • 別名:
    Mas-related G-protein coupled receptor MRG (MAS-R) (MAS1-like), MAS1L, MRG
  • 反應種屬:
    Human, Rat
  • 免疫原:
    A synthesized peptide derived from human MAS1L
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    Rabbit IgG
  • 純化方式:
    Affinity-chromatography
  • 克隆號:
    15B8
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB, IF, FC
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 基因功能參考文獻:
    1. Mas, MrgD, and MRG mediated Ang IV-stimulated AA release that was highest for Mas. While Ang III activated Mas and MrgX2, Ang II stimulated AA release via Mas and MRG. PMID: 18636314
  • 亞細胞定位:
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族:
    G-protein coupled receptor 1 family, Mas subfamily
  • 數據庫鏈接:

    HGNC: 13961

    OMIM: 607235

    KEGG: hsa:116511

    STRING: 9606.ENSP00000366331

    UniGene: Hs.533297



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