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LDHC Recombinant Monoclonal Antibody

  • 中文名稱:
    LDHC重組抗體
  • 貨號:
    CSB-RA886298A0HU
  • 規格:
    ¥1320
  • 圖片:
    • Overlay Peak curve showing Hela cells stained with CSB-RA886298A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他:

產品詳情

  • 產品描述:
    CSB-RA886298A0HU LDHC重組單克隆抗體是針對乳酸脫氫酶C亞基(LDHC)開發的高特異性科研試劑。該抗體通過重組蛋白免疫策略制備,經ELISA和流式細胞術(FC)嚴格驗證,在FC應用中推薦使用1:50-1:200稀釋比例。LDHC作為糖酵解關鍵酶,特異性地表達于生殖細胞及多種腫瘤組織,其異常表達與精子活力調控及腫瘤代謝重編程密切相關。本產品能特異性識別天然構象的LDHC蛋白,在FC檢測中展現出優異的信號分辨率和低背景特性,適用于活細胞表面標記及胞內蛋白定位分析。該抗體具有高批次穩定性,可為生殖生物學研究(如精子功能評估)、腫瘤微環境代謝研究(包括腫瘤細胞能量代謝通路解析)以及生殖系統相關疾病機制探索提供可靠工具。其優化的FC性能特別適合用于流式細胞術檢測中低豐度靶標的精準識別,為科研人員開展細胞水平的功能研究提供有效支持。
  • Uniprot No.:
  • 基因名:
  • 別名:
    L-lactate dehydrogenase C chain (LDH-C) (EC 1.1.1.27) (Cancer/testis antigen 32) (CT32) (LDH testis subunit) (LDH-X), LDHC, LDH3 LDHX
  • 反應種屬:
    Human
  • 免疫原:
    A synthesized peptide derived from human LDHC
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    Rabbit IgG
  • 純化方式:
    Affinity-chromatography
  • 克隆號:
    9F2
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, FC
  • 推薦稀釋比:
    Application Recommended Dilution
    FC 1:50-1:200
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    Possible role in sperm motility.
  • 基因功能參考文獻:
    1. Lactate dehydrogenase C may act as a novel biomarker for renal cell carcinoma progression and a potential therapeutic target for the treatment of renal cell carcinoma. PMID: 28351304
    2. three uniquely identified proteins (CDK6 , galectin-3-binding protein and LDH C) were found, which show tight connection with prostate cancer and presence of all of them was previously linked to certain aspects of prostate cancer PMID: 26503549
    3. Down-regulated LDH-C4 expression is significantly correlated with lowered enzyme activity in human spermatozoa. PMID: 25795631
    4. This is the first report to show a positive correlation between LDH-3 and hemolytic parameters in sickle cell anemia. PMID: 26069337
    5. LDH3 was increased in essential thrombocythemia. This isoenzymatic pattern could be expression of a metabolic adaptation. PMID: 17178662
    6. LDH3 is a supporting diagnostic marker in cases of chronic tuberculosis. PMID: 17935709
    7. hLdhc expression in cancer cells was regulated by transcription factor Sp1 and CREB and promoter CGI methylation PMID: 18930904

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  • 亞細胞定位:
    Cytoplasm.
  • 蛋白家族:
    LDH/MDH superfamily, LDH family
  • 數據庫鏈接:

    HGNC: 6544

    OMIM: 150150

    KEGG: hsa:3948

    STRING: 9606.ENSP00000280704

    UniGene: Hs.654377



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