HLA-B&HLA-A Recombinant Monoclonal Antibody
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中文名稱:HLA-B&HLA-A Recombinant Monoclonal Antibody
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貨號:CSB-RA829498A0HU
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規格:¥1320
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圖片:
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Western Blot
Positive WB detected in: Hela whole cell lysate(30μg), A431 whole cell lysate(30μg), HEK293 whole cell lysate(30μg)
All lanes: HLA-A antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time:1min -
Overlay Peak curve showing Raji cells stained with CSB-RA829498A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他:
產品詳情
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Uniprot No.:
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基因名:HLA-B&HLA-A
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別名:HLA class I histocompatibility antigen, A-3 alpha chain (MHC class I antigen A*3), HLA-A, HLAA; HLA class I histocompatibility antigen, B-7 alpha chain (MHC class I antigen B*7), HLA-B, HLAB
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反應種屬:Human
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免疫原:A synthesized peptide from human HLA-B&HLA-A protein
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免疫原種屬:Homo sapiens (Human)
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標記方式:Non-conjugated
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克隆類型:Monoclonal
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抗體亞型:Rabbit IgG
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純化方式:Affinity-chromatography
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克隆號:5A12
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式:Liquid
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應用范圍:ELISA, WB, FC
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推薦稀釋比:
Application Recommended Dilution WB 1:500-1:2000 FC 1:50-1:200 -
Protocols:
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儲存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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