在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. Home
  2. 抗體
  3. 重組抗體
  4. GUSB Recombinant Monoclonal Antibody

GUSB Recombinant Monoclonal Antibody

  • 中文名稱：
    GUSB Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA576809A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: THP-1 whole cell lysate(30μg), U-251MG whole cell lysate(30μg), A431 whole cell lysate(30μg), HeLa whole cell lysate(30μg), HepG2 whole cell lysate(30μg)
      All lanes: GUSB antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 75 kDa
      Observed band size: 78 kDa
      Exposure time：3min
    • IHC image of CSB-RA576809A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA576809A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of MCF-7 cell with CSB-RA576809A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing 786-O cells stained with CSB-RA576809A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    Beta-glucuronidase (EC 3.2.1.31) (Beta-G1), GUSB
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide from human GUSB protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    11D12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays an important role in the degradation of dermatan and keratan sulfates.
  • 基因功能參考文獻(xiàn)：
    1. Results show that GUS mRNA changes in blood are significantly correlated with gait step length variability in premutation (PM) females patients with fragile X tremor ataxia syndrome . PMID: 27387142
    2. We produced GUS from a CHO cell line grown in suspension in a 15 L perfused bioreactor and developed a three step purification procedure that yields approximately 99% pure enzyme with a recovery of more than 40%. PMID: 28734840
    3. Selective and amplified bG expression together with the prodrug DOXGA3 had an increased antitumor effect, showing great potential for prostate cancer therapy. PMID: 26648021
    4. the use of in silico approaches provided a useful understanding of the effect of single point mutations on the structure-function relationship of GUSBp PMID: 26415878
    5. the efficacy of a helper-dependent (HD) canine adenovirus (CAV-2) vector harboring a human GUSB expression cassette (HD-RIGIE) in the MPS VII dog brain was tested PMID: 24343103
    6. This assessment demonstrated that although butyrate dramatically increased beta-glucuronidase production in bioreactors, it adversely impacted the mannose-6-phosphorylation of this lysosomal storage diseasestherapeutic enzyme PMID: 24033810
    7. expression of CES2, UGTA1A1, and GUSB varies in colorectal pathology tissues and that the expression of CES2 is somewhat related to tumor staging. PMID: 24195516
    8. GUSB and ATP2B4 have been validated as a reliable gene combination for Cystic Fibrosis Transmembrane Conductance Regulator gene qPCR data normalization. PMID: 22525089
    9. Liver disease affects the expression of common HKGs; beta-glucuronidase and splicing factor arginine/serine-rich 4 are the most stable HKGs for studies of gene expression in HCV-infected human liver. PMID: 21073651
    10. beta-glucuronidase mutations are associated with mucopolysaccharidosis type VII PMID: 12522561
    11. Recombinant adeno-associated virus encoding human GUSB injected into the vitreous humor of young adult MPS VII mice increased GUSB activity and reduced lysosomal distension in regions of the thalamus and tectum PMID: 12716937
    12. beta glucuronidase present in the synovial fluid of rheumatoid arthritis patients, may contribute to the depletion of glycosaminoglycans from cartilage allowing invasion of synovial cells. PMID: 12905469
    13. Over-expression of beta-glucuronidase was related to the degree of cancer differentiation, but not to lymph node metastasis. PMID: 14612292
    14. These results indicate that beta-glucuronidase transport into brain parenchyma in early postnatal life is mediated by the mannose 6-phosphate/insulin-like growth factor II receptor. PMID: 15314220
    15. The genes GUS and PMM1 are recommended for normalization purposes in gene expression studies of liver tissue from patients with chronic hepatitis. PMID: 18591914
    16. Study summarized information on the 49 unique, disease-causing mutations determined so far in the GUS gene. PMID: 19224584

    顯示更多

    收起更多

  • 相關(guān)疾病：
    Mucopolysaccharidosis 7 (MPS7)
  • 亞細(xì)胞定位：
    Lysosome.
  • 蛋白家族：
    Glycosyl hydrolase 2 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 4696

    OMIM: 253220

    KEGG: hsa:2990

    STRING: 9606.ENSP00000302728

    UniGene: Hs.255230



主站蜘蛛池模板： 五月丁香六月综合缴情在线| 欧美高清性色生活片免费观看| 亚洲欧美一区二区三区在线| 国产成人啪精品视频免费网站软件| 亚色九九九全国免费视频| 婷婷综合缴情亚洲狠狠小说| 日韩精品久久无码中文字幕| 99re6热在线精品视频观看| 精品一区二区三区国产在线观看| 亚洲精品无码不卡在线播放| 欧美颜射内射中出口爆在线| 在线天堂新版最新版在线8| 2021国产精品香蕉在线观看| 色欲网天天无码av| 久久久久久综合网天天| 天天摸日日摸爽爽狠狠| 少妇爱做高清免费视频| 无码少妇一区二区性色av| 少妇邻居内射在线| 成人无码男男gv在线观看网站| 久久精品www人人做人人爽| 国产av熟女一区二区三区| 精品久久久久久无码国产| 中文av伊人av无码av狼人| 特黄aaaaaaa片免费视频| 国产成人无码视频一区二区三区| 最新的国产成人精品2021| 最美女人体内射精一区二区| 国产高颜值大学生情侣酒店| 精品无人区一区二区三区| 中文国产成人精品久久久| 久久亚洲精品中文字幕无码| 少妇把腿扒开让我爽爽视频| 国产精品一区二区在线蜜芽tv| 国产精品 精品国内自产拍| 久久精品免费一区二区| 免费精品国偷自产在线在线 | 亚洲综合网站久久久| 国产精品一区二区无线| 无码av波多野结衣| 国产美女自卫慰水免费视频|