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GRIN1 Recombinant Monoclonal Antibody

  • 中文名稱:
    GRIN1重組抗體
  • 貨號:
    CSB-RA904344A0HU
  • 規格:
    ¥1320
  • 圖片:
    • Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA904344A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他:

產品詳情

  • 產品描述:
    CSB-RA904344A0HU為GRIN1重組單克隆抗體,專為精準檢測谷氨酸受體NMDA型亞基1(GRIN1)設計。該抗體靶向的GRIN1是中樞神經系統中關鍵的離子型谷氨酸受體亞基,參與調控突觸可塑性、學習記憶等生理過程,其功能異常與神經退行性疾病及精神類疾病密切相關。本產品采用重組表達技術制備,經ELISA和流式細胞術(FC)嚴格驗證,展現出優異的目標蛋白結合特異性與批次穩定性。實驗數據顯示,在流式細胞分析中推薦使用1:50-1:200稀釋比例,可高效檢測細胞內源性GRIN1表達水平,適用于神經元分化研究、受體分布分析以及相關信號通路機制探索。科研人員可將其用于體外培養的神經細胞模型、腦組織原代細胞等樣本的蛋白定位及定量檢測,為神經科學領域的基礎研究提供可靠工具。該抗體不適用于活體實驗或蛋白印跡檢測。
  • Uniprot No.:
  • 基因名:
  • 別名:
    Glutamate receptor ionotropic, NMDA 1 (GluN1) (Glutamate [NMDA] receptor subunit zeta-1) (N-methyl-D-aspartate receptor subunit NR1) (NMD-R1), GRIN1, NMDAR1
  • 反應種屬:
    Human
  • 免疫原:
    A synthesized peptide derived from Human GRIN1
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    Rabbit IgG
  • 純化方式:
    Affinity-chromatography
  • 克隆號:
    24F5
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, FC
  • 推薦稀釋比:
    Application Recommended Dilution
    FC 1:50-1:200
  • Protocols:
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能:
    Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition.
  • 基因功能參考文獻:
    1. Glycans potentiate the effect of GluN1 and GluN2B receptors. PMID: 28378791
    2. In this study, we report a success of the WES approach to the identification of a genetic cause of a challenging case, undiagnosed on clinical grounds. We identified the causative missense mutation (p.Met727Val) in exon 16 of the GRIN1 gene. As the p.Met727Val mutation shown by WES is in the same GluN1 domain, we infer that the pathogenic variant impact on NMDAR is likely similar to that induced by p.Glu662Lys mutation. PMID: 29194067
    3. one base difference in the GRIN1M promoter sequence (G --> C) results in the inability of the sequence to form a parallel G-quadruplex. PMID: 28702665
    4. Data suggest GRINL1A (GCOM1)-NMDA receptor-internexin-alpha (INA) interaction pathway may be relevant to neuroprotection. PMID: 29339073
    5. These results indicate these individuals may have suffered neurodevelopmental deficits as a result of the decreased presence of GluN1-G620R/GluN2B complexes on the neuronal surface during embryonic brain development and reduced current responses of GluN1-G620R-containing NMDARs after birth. PMID: 28228639
    6. Mice with GRIN1 disrupted in the intralaminar thalamic nuclei exhibited various schizophrenia-like phenotypes, including deficits in working memory, long-term spatial memory, and attention, as well as impulsivity, impaired prepulse inhibition, hyperlocomotion and hyperarousal. PMID: 28244984
    7. 2-methoxyestradiol impacts on glycine/serine-mediated metabolic reprogramming in osteosarcoma cells by its interaction with GRIN1/GluN2A receptors. PMID: 28262924
    8. tPA is a ligand of the N-terminal domain of the obligatory GluN1 subunit of NMDAR acting as a modulator of their dynamic distribution at the neuronal surface and subsequent signaling. PMID: 27831563
    9. Two novel Grin1 mutations were identified in 2 cases of severe early infantile encephalopathy. Se688Tyr mutation results in disruption of NMDA ligand binding and the p.Gly827Arg mutation results in disrupted gating of the ion channel. PMID: 28389307
    10. A homozygous missense variant of GRIN1 was identified in two consanguineous sibs affected with severe intellectual disability and autistic features. PMID: 28051072
    11. NMDA receptor-dependent signaling is involved in melanosome transfer, which is associated with calcium influx, cytoskeleton protein redistribution, dendrites and filopodia formation PMID: 27596138
    12. Findings show that N-methyl-d-aspartic acid receptor subunit GluN1 is expressed on oligodendrocytes and myelin in humans. PMID: 27443784
    13. De novo GRIN1 mutations are associated with severe intellectual disability with cortical visual impairment as well as oculomotor and movement disorders being discriminating phenotypic features. Loss of NMDA receptor function appears to be the underlying disease mechanism. The identification of both heterozygous and homozygous mutations blurs the borders of dominant and recessive inheritance of GRIN1-associated disorders. PMID: 27164704
    14. The differences in cortical NMDAR expression and post-synaptic density protein 95 are present in psychiatric disorders and suicide completion and may contribute to different responses to ketamine. PMID: 26013316
    15. GRIN1 (rs4880213) was significantly associated with depression and disruptive behavior in adolescents. PMID: 26819771
    16. Knockdown of PKD1 did not affect NMDAR internalization but prevented the phosphorylation and inhibition of remaining surface NMDARs and NMDAR-mediated synaptic functions. PMID: 26584860
    17. Study found GluN receptor subunit-specific changes in mixed subcortical ischemic vascular dementia(SIVD)/Alzheimer's disease(AD) (decreased GluN1) and SIVD (increased GluN2A and 2B), likely reflecting interaction of ischemic neurovascular and AD processes PMID: 25261450
    18. results suggest that NMDA-R autoantibodies are unlikely to account for a large proportion of treatment-refractory psychosis. PMID: 25431428
    19. The results of this study suggested that GRIN1 mutations cause encephalopathy resulting in seizures and movement disorders. PMID: 25864721
    20. Genome-wide significant marker, SNP rs524991, and an association of seropositivity with influenza autoantibodies status, we provide genetic and environmental risk factors of NMDAR-autoantibodies formation PMID: 23999527
    21. Epigenetic changes in GRIN1, in combination with experiences of maltreatment, may confer risk for depression in children. PMID: 24655651
    22. Reduction in NR1 and NR2C in the DLPFC of people with schizophrenia may lead to altered NMDAR stoichiometry and provides compelling evidence for an endogenous NMDAR deficit in schizophrenia. PMID: 23070074
    23. Isolated GluN1/GluN3A receptors integrated into lipid bilayers responded to addition of either glycine or d-serine, but not glutamate, with a approximately 1 nm reduction in height of the extracellular domain PMID: 25017909
    24. Results show that the expression and distribution of NMDA receptors subunits GluN1, GluN2A and GluN2B - together with that of postsynaptic protein PSD-95 - are modified in Alzheimer's disease compared to normal aging PMID: 24156266
    25. B7T inhibition of NMDA current mediated by NR1/NR2B receptor. PMID: 23271275
    26. The rs1126442, GRIN1 polymorphism contributes to the genetic vulnerability to psychosis in METH-dependent subjects in the Thai population. PMID: 23880023
    27. Association of multiple sclerosis disease severity and allelic variants of the NR1 and NR2B glutamate receptor genes. PMID: 23840674
    28. GluN1 binds specifically to the sigma-1 receptor within intact cells. PMID: 24227730
    29. Antibodies that bind recombinant GluN1-S2 peptides (but not the intact GluN1 protein) develop transiently in patients after stroke in proportion to infarct size, suggesting that these antibodies are raised secondarily to neuronal damage. PMID: 23723305
    30. Transgenic NR1 receptors on neuradrenergic neurons regulate development of opiate dependence and psychomotor sensitization. PMID: 22040728
    31. After 7 days of chronic alcohol exposure, there are significant increases in mRNA expression of GRIN1 in cultured neurons derived from alcoholic subjects, but not in cultures from nonalcoholics. PMID: 22486492
    32. Adult NR1-deficient transgenic mice show multiple abnormal behaviors including reduced social interactions, locomotor hyperactivity, self-injury, deficits in prepulse inhibition and sensory hypersensitivity, among others. PMID: 22726567
    33. GRIN1 and GRIN2D appear instrumental to normal brain development and function in this study of rare and/or de novo mutation in neurodevelopmental disorders. PMID: 22833210
    34. The multifunctional cytokine-like molecule HMGB1 released by activated, stressed, and damaged or necrotic cells can facilitate NMDAR-mediated cell responses. PMID: 22952988
    35. A critical role of the single glutamine residue within the GluN1 M4 domain regulates surface delivery of functional NMDA receptors. PMID: 22937865
    36. key amino acid residues within both NR1 and NR2B M3 domains contribute to the regulation of the surface expression of unassembled NR1 and NR2 subunits PMID: 22711533
    37. The unique co-existence of SP and phospho-NMDAR1 in tendinopathy presumably reflects a tissue proliferative and nociceptive role. PMID: 22354721
    38. GluN1(hypo) transgenic mice exhibit impairments on all tests of cognition that are employed, as well as reduced engagement in naturalistic behaviors, including nesting and burrowing. PMID: 22300668
    39. The NR1 subunit of NMDA receptors is involved in amygdala hyperexcitability in some patients who have temporal lobe epilepsy. PMID: 20848605
    40. G Protein-regulated inducer of neurite outgrowth (GRIN) modulates Sprouty protein repression of mitogen-activated protein kinase (MAPK) activation by growth factor stimulation PMID: 22383529
    41. Transgenic mice with dopaminergic neuron-specific NMDAR1 deletion are impaired in a variety of habit-learning tasks, while normal in some other dopamine-modulated functions such as locomotor activities. PMID: 22196339
    42. Homozygotes for the T allele in the rs4880213 GRIN1 SNP had reduced intracortical inhibition, as expected for enhanced glutamatergic excitation in these subjects. PMID: 21753020
    43. NMDAR1 subunit expressed by primary afferent nerves of floxed mice plays an important role in the development of sensitized pain states. PMID: 20974228
    44. Expression of NMDA receptors in lymphocytes is regulated by central nervous system, which controls the inflammation process. PMID: 20414717
    45. The results of this study suggested that haplotypes of GRIN1 may influence responsiveness to ACTH. PMID: 20722663
    46. Sp4 hypomorphic mice could therefore serve as a genetic model to investigate impaired NMDA functions resulting from loss-of-function mutations of human SP4 gene in schizophrenia and/or other psychiatric disorders. PMID: 20634195
    47. Both tissue-type PA (tPA) and urokinase-type PA (uPA) bind to NMDA-R1 and reverse this effect, thereby enhancing acetylcholine-induced tracheal contractility. PMID: 20097831
    48. Functional NMDA receptors are expressed by breast cancer and are important agents for maintaining cell growth and viability. PMID: 19784770
    49. Polymorphisms in the GRIN1 and GRIN2B genes may serve as potential biomarkers for a reduced risk of PD among the Chinese population in Taiwan. PMID: 20438806
    50. The neuronal coexistence of glutamate and NMDAR1, observed in painful tendinosis but not in controls, suggests a regulatory role in intensified pain signalling. PMID: 19422642

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  • 相關疾病:
    Neurodevelopmental disorder with or without hyperkinetic movements and seizures, autosomal dominant (NDHMSD)
  • 亞細胞定位:
    Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane. Cell junction, synapse, postsynaptic density.
  • 蛋白家族:
    Glutamate-gated ion channel (TC 1.A.10.1) family, NR1/GRIN1 subfamily
  • 數據庫鏈接:

    HGNC: 4584

    OMIM: 138249

    KEGG: hsa:2902

    STRING: 9606.ENSP00000360608

    UniGene: Hs.558334



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