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  4. GOPC Recombinant Monoclonal Antibody

GOPC Recombinant Monoclonal Antibody

  • 中文名稱(chēng)：
    GOPC Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA210187A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HepG2 whole cell lysate(30μg),PC-3 whole cell lysate(30μg), Hela whole cell lysate(30μg),THP-1 whole cell lysate(30μg),K562 whole cell lysate(30μg),Rat brain tissue lysate(20μg)
      All lanes: GOPC antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 51 kDa
      Observed band size: 59 kDa
      Exposure time：3min
    • IHC image of CSB-RA210187A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA210187A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing Hela cells stained with CSB-RA210187A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    GOPC
  • 別名：
    CAL antibody; CFTR associated ligand antibody; CFTR-associated ligand antibody; dJ94G16.2 antibody; dJ94G16.2 PIST antibody; FIG antibody; Fused in glioblastoma antibody; Golgi associated PDZ and coiled coil motif containing antibody; Golgi associated PDZ and coiled coil motif containing protein antibody; Golgi-associated PDZ and coiled-coil motif-containing protein antibody; GOPC 1 antibody; GOPC antibody; GOPC_HUMAN antibody; GOPC1 antibody; OTTHUMP00000040403 antibody; PDZ protein interacting specifically with TC 10 antibody; PDZ protein interacting specifically with TC10 antibody; PDZ/coiled coil domain binding partner for the rho family GTPase TC 10 antibody; PDZ/coiled coil domain binding partner for the rho family GTPase TC10 antibody; PIST antibody; Protein interacting specifically with Tc 10 antibody; Protein interacting specifically with Tc10 antibody
  • 反應(yīng)種屬：
    Human, Rat
  • 免疫原：
    A synthesized peptide derived from human GOPC
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類(lèi)型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    4B10
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.
  • 基因功能參考文獻(xiàn)：
    1. PIST, a Golgi-associated PDZ domain-containing protein, interacts with PCDH15 in the plasma membrane. PMID: 27867666
    2. CAL can regulate the anterograde trafficking, the internalization as well as the signaling of CRFR1 via modulating the post-translational modifications that the receptor undergoes at the Golgi apparatus. PMID: 26115868
    3. CAL overexpression retards B2AR expression in Golgi apparatus and reduces the receptor expression in the plasma membrane. PMID: 25876703
    4. Six out of 65 (9%) BTC patients were positive for the FIG-ROS1 fusion, comprising two out of 14 (14%) gallbladder carcinoma (GBC) patients and four out of 25 (16%) extrahepatic cholangiocarcinoma (ECC) patients. PMID: 25231053
    5. Our data suggest that PIST contributes to the fine-tuning of b1AR sorting both during biosynthetic and postendocytic trafficking PMID: 25614626
    6. None of 268 gliomas analysed showed the FIG-ROS1(L) rearrangement. PMID: 24999209
    7. Stereochemical preferences modulate affinity and selectivity among CAL PDZ domain protein variants that bind CFTR. PMID: 24210758
    8. FIG-ROS fusion protein, which is found in a subset of intrahepatic cholangiocarcinoma patients, accelerates cholangiocarcinogenesis. PMID: 24154728
    9. Data indicate that E3 ubiquitin ligase MARCH2 co-immunoprecipitated and co-localized with CAL and syntaxin 6 (STX6), and show the ubiquitination of CFTR by MARCH2. PMID: 23818989
    10. The coiled-coil domain of PIST was then purified to homogeneity and crystallized at 293 K. Finally, X-ray diffraction data were collected to a resolution of 4.0 A from a crystal belonging to the hexagonal space group P6(2)22 or P6(4)22. PMID: 23545662
    11. crystals of CAL PDZ domain diffracted to 1.4 A resolution PMID: 21543871
    12. establish CAL inhibitors as founding members of a class of CFTR "stabilizers" specifically designed to reduce DeltaF508-CFTR post-endocytic breakdown PMID: 21105033
    13. Syntaxin 6 and CAL mediate the degradation of the cystic fibrosis transmembrane conductance regulator PMID: 20130090
    14. The FIG-ROS locus encodes for an in-frame fusion protein with a constitutively active kinase activity, suggesting that FIG-ROS may act as an oncogene. PMID: 12661006
    15. CAL retains CFTR in the cell and targets CFTR for degradation. PMID: 14570915
    16. modulates beta1AR intracellular trafficking PMID: 15358775
    17. golgin-160 assosiates in an isoform-specific manner with the Golgi-associated protein PIST PMID: 15951434
    18. intracellular sorting of the somatostatin receptor subtype 5 is regulated by interactions with PDZ domain proteins PIST/GOPC and PDZK1 PMID: 16012170
    19. Our discoveries provide insight into the specific interaction of the GOPC PDZ domain with the C-terminal peptide of Nlg and also provide a general insight about the possible binding mode of the interaction of Nlg with other PDZ domain-containing proteins. PMID: 16882988
    20. CAL is a negative regulator of DeltaF508-CFTR cell-surface expression PMID: 17158866
    21. The cystic fibrosis-associated ligand (CAL) PDZ domain of the C-terminus of cystic fibrosis-associated conductance regulator (CFTR) is competitively involved with PDZ proteins Na+/H+ exchanger-3 regulatory factors NHERF1 and NHERF2. PMID: 18754678

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  • 相關(guān)疾?。?/div>
    A chromosomal aberration involving GOPC is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
  • 亞細(xì)胞定位：
    Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Cell junction, synapse. Cell junction, synapse, postsynaptic density. Cell projection, dendrite.
  • 組織特異性：
    Ubiquitously expressed.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 17643

    OMIM: 606845

    KEGG: hsa:57120

    STRING: 9606.ENSP00000357484

    UniGene: Hs.191539



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