GLUD1 Recombinant Monoclonal Antibody
-
中文名稱:GLUD1 Recombinant Monoclonal Antibody
-
貨號:CSB-RA938641A0HU
-
規格:¥1320
-
圖片:
-
IHC image of CSB-RA938641A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
-
IHC image of CSB-RA938641A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
-
Overlay Peak curve showing PC-3 cells stained with CSB-RA938641A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
-
-
其他:
產品詳情
-
Uniprot No.:
-
基因名:
-
別名:Glutamate dehydrogenase 1, mitochondrial (GDH 1) (EC 1.4.1.3), GLUD1, GLUD
-
反應種屬:Human
-
免疫原:A synthesized peptide from human GLUD1 protein
-
免疫原種屬:Homo sapiens (Human)
-
標記方式:Non-conjugated
-
克隆類型:Monoclonal
-
抗體亞型:Rabbit IgG
-
純化方式:Affinity-chromatography
-
克隆號:16B7
-
濃度:It differs from different batches. Please contact us to confirm it.
-
保存緩沖液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式:Liquid
-
應用范圍:ELISA, IHC, FC
-
推薦稀釋比:
Application Recommended Dilution IHC 1:50-1:200 FC 1:50-1:200 -
Protocols:
-
儲存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
-
貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
-
用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
相關產品
靶點詳情
-
功能:Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
-
基因功能參考文獻:
- This study provides insight into the molecular mechanism by which GDH1-mediated metabolic reprogramming of glutaminolysis mediates lung cancer metastasis and offers a therapeutic strategy for patients with LKB1-deficient lung cancer. PMID: 29249655
- Phenotypic heterogeneity of GDH-hyperinsulinemia patients was observed within the Chinese cohort in the present study. The fact that most patients had a GLUD1 p. S445L mutation implies that this site could be a hotspot in Chinese patients. PMID: 30306091
- structural model of a complex of human Sirt4 and GDH in order to elucidate the molecular mechanism underlying the ADP-ribosylation of GDH by Sirt4 PMID: 29571013
- Upregulation of GLUD1 and SLC25A13 was associated with tumor aggressiveness and poorer prognosis in colorectal cancer patients. PMID: 27924922
- This study showed that SIRT5 supports the anaplerotic entry of glutamine into the TCA cycle in malignant phenotypes of CRC via activating GLUD1. PMID: 29416026
- the present study shows that the GDHS445L enzyme is highly sensitive to the allosteric activator ADP, explaining the hyperactivity of this mutant. INS-1E beta-cells expressing GDHS445L responded to glutamine stimulation by increasing the mitochondrial respiration and ATP production, as well as by a rise in cytosolic calcium. PMID: 28911206
- We have shown that alpha-ketoglutarate substrate inhibition kinetics of GDH, which include both random and obligatory ordered association/dissociation reactions, robustly control the ratio between glutamate and ammonium under a wide range of intracellular substrate variation. Dysregulation of this activity under pericentral nitrogen insufficiency contributes to the breaking down of ammonia homeostasis and thereby can s... PMID: 27747338
- mRNA and protein levels of GluD1 were increased in iPSC-derived neurons from FOXG1(+/-) patients. PMID: 26443267
- Hypoxia-induced expression of GDH relies on the up-regulation of HIF1alpha but not HIF2alpha. HIF1alpha binds the promoter of GDH and promotes the transcription of GDH gene in lung cancer cells. PMID: 28065856
- Results indicate that it is possible to use high-throughput screening methods to find activators for glutamate dehydrogenase (GDH) that might be useful as pharmaceutical agents. PMID: 27808506
- Missense mutation of GLUD1 is associated with Hyperinsulinism-hyperammonemia syndrome. PMID: 27383869
- Analysis of Serbian patients with Hyperinsulinism/hyperammonemia syndrome confirms the association of p.S445L and p.R221C mutations with hypoglycaemic seizures noted within the first three months of life and with subsequent risk for cognitive impairment and/or epilepsy. PMID: 26759084
- GLUD1 is differentially expressed in the cellular and subcellular compartments of numerous tissues. PMID: 27422263
- GLUD1 mutation is associated with congenital hyperinsulinism-hyperammonemia. PMID: 26656609
- Study of the expression of the GDH1/2 in human steroidogenic organs revealed that, while GDH2 was expressed specifically in steroid-synthesizing cells, GDH1 was expressed both in the cells that produce steroids and in those that lack endocrine function. PMID: 26241911
- GDH plays a critical role in colorectal cancer progression PMID: 25947346
- Mutation in the GLUD1 gene is associated with hyperinsulinism/hyperammonemia. PMID: 25781533
- side-chain interactions between 409 and 443 positions in the 'antenna' region of GDH are crucial for basal catalytic activity, allosteric regulation, and relative resistance to thermal inactivation PMID: 25620628
- argeting GDH1 by shRNA or a small molecule inhibitor R162 resulted in imbalanced redox homeostasis, leading to attenuated cancer cell proliferation and tumor growth. PMID: 25670081
- SIRT3 and glutamate dehydrogenase are altered in follicular cells of women with reduced ovarian reserve or advanced maternal age PMID: 24771001
- The GDH1 is a key metabolic enzyme with emerging roles in insulin regulation. MitoNEET forms a covalent complex with GDH1 through disulfide bond formation and acts as an activator. PMID: 24295216
- The data suggest a dual mechanism by which glutamate dehydrogenase activity modulates autophagy, i.e., by activating MTORC1 and by limiting the formation of reactive oxygen species. PMID: 23575388
- first N-terminal alpha helical structure is crucial for the mitochondrial import of hGDH2 and these findings may have implications in understanding the evolutionary mechanisms that led to the large mitochondrial targeting signals of human GDHs PMID: 22658952
- Lack of Glud1 in transgenic mouse brain inhibits GDH activity regardless of enzymatic direction, modified glutamate handling, decreased glutamate catabolism and increased glutamine levels without affecting synaptic transmission. PMID: 22924626
- Transgenic mice are useful in defining the molecular pathways affected by the over-activation of GLUD transgene in glutamatergic neurons of the brain and spinal cord. PMID: 21397652
- Human GDH1 appears to act like bovine GDH1, but human GDH2 does not show the same enhancement of branched chain alpha-keto acid dehydrogenase complex enzyme activities. PMID: 21621574
- [review] Hyperinsulinemic hypoglycemia and hyperammonemia demonstrate the important role of GDH in insulin regulation and illustrate unexpectedly important reasons for the unusually complex allosteric regulation of GDH. PMID: 21130127
- GDH interactions with aristolochic acid induce apoptosis of renal tubular epithelial cells. PMID: 21901531
- Report glutamate dehydrogenase mutations in Japanese patients with congenital hyperinsulinism and hyperammonemia syndrome. PMID: 22106762
- Green tea polyphenols control dysregulated glutamate dehydrogenase in transgenic mice by hijacking the ADP activation site. PMID: 21813650
- In Chinese, R269H, S445L mutation of the GLUD1 leads to congenital hyperinsulinism. PMID: 20931523
- Gradual decrease in leukocyte GLDH activity may be a key factor for neurodegenerative aging processes. PMID: 20197649
- Two missense mutations give rise to two different forms of congenital hyperinsulinism/hyperammonemia. PMID: 11840195
- increased glutamate dehydrogenase activity confers on beta-cells the ability to secrete insulin in response to glutamine PMID: 11872671
- Expression, purification and characterization of human glutamate dehydrogenase (GDH) allosteric regulatory mutations PMID: 11903050
- identify the structural basis for allosteric differences of GlUD1 and GLUD2 PMID: 11950837
- Tyr187 is responsible for the efficient base binding of ADP to human GDH. PMID: 12022886
- site-directed mutagenesis showing involvement of Glu(279)in NAD+ binding PMID: 12193607
- substitution of Ser for Arg-443 (but not substitution of Thr for Ser-331, Leu for Met-370, or Leu for Met-415) virtually abolished basal activity and totally abrogated the activation of the enzyme by l-leucine (1-10 mm) in the absence of other effectors PMID: 12324473
- These results suggest that the Ser443 residue plays an important role in the different thermal stability of human glutamate dehydrogenase isozymes (hGDH1 and hGDH2). PMID: 15044002
- inhibition of glutamate dehydrogenase results in sulfite neurotoxicity PMID: 15273247
- GLUD2 originated by retroposition from GLUD1 in the hominoid ancestor less than 23 million years ago. PMID: 15378063
- results suggest that cysteine 323 plays an important role in catalysis by human GDH isozymes; C323 is not directly involved in allosteric regulation. PMID: 15750346
- GLUD1 may have a role in hyperinsulinism/hyperammonaemia syndrome PMID: 16151898
- Results describe the electrophysiological mechanisms underlying the dysregulated insulin secretion in pancreatic beta cells with glutamate dehydrogenase mutations. PMID: 16492972
- we describe a family with a dominantly inherited mutation in GDH in Myoclonic absence epilepsy with photosensitivity. PMID: 18321734
- GLUD1 parental protein localizes to mitochondria and the cytoplasm, GLUD2 is specifically targeted to mitochondria. PMID: 18688271
- Although GDH activity is also highly expressed in the brain, central nervous system (CNS) manifestations of GLUD1 activating mutations have remained relatively unexplored. PMID: 19046183
- Neurological disorders in HHS are more frequent than previously thought and might suggest that mutations in the GTP binding site of GDH could be associated with more frequent epilepsy. PMID: 19046187
- Hence, while most of the hGDHs translocate into the mitochondria (a process associated with cleavage of the signal sequence), part of the protein localizes to the endoplasmic reticulum, probably serving additional functions PMID: 19448744
顯示更多
收起更多
-
相關疾病:Familial hyperinsulinemic hypoglycemia 6 (HHF6)
-
亞細胞定位:Mitochondrion. Endoplasmic reticulum.
-
蛋白家族:Glu/Leu/Phe/Val dehydrogenases family
-
數據庫鏈接:
Most popular with customers
-
-
YWHAB Recombinant Monoclonal Antibody
Applications: ELISA, WB, IHC, IF, FC
Species Reactivity: Human, Mouse, Rat
-
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody
Applications: ELISA, WB, IHC
Species Reactivity: Human
-
-
-
-
-