GATAD2A Recombinant Monoclonal Antibody
-
中文名稱:GATAD2A Recombinant Monoclonal Antibody
-
貨號(hào):CSB-RA256477A0HU
-
規(guī)格:¥1320
-
圖片:
-
Western Blot
Positive WB detected in:K562 whole cell lysate(30μg), Jurkat whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg)
All lanes: p66 alpha antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time:1min -
IHC image of CSB-RA256477A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
-
Immunofluorescence staining of MCF-7 cell with CSB-RA256477A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
-
Overlay Peak curve showing jurkat cells stained with CSB-RA256477A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
-
-
其他:
產(chǎn)品詳情
-
Uniprot No.:
-
基因名:GATAD2A
-
別名:Transcriptional repressor p66-alpha (Hp66alpha) (GATA zinc finger domain-containing protein 2A), GATAD2A
-
反應(yīng)種屬:Human, Mouse
-
免疫原:A synthesized peptide from human GATAD2A protein
-
免疫原種屬:Homo sapiens (Human)
-
標(biāo)記方式:Non-conjugated
-
克隆類型:Monoclonal
-
抗體亞型:Rabbit IgG
-
純化方式:Affinity-chromatography
-
克隆號(hào):12B10
-
濃度:It differs from different batches. Please contact us to confirm it.
-
保存緩沖液:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式:Liquid
-
應(yīng)用范圍:ELISA, WB, IHC, IF, FC
-
推薦稀釋比:
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 -
Protocols:
-
儲(chǔ)存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
-
貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
-
用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
-
功能:Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B.
-
基因功能參考文獻(xiàn):
- The MYND domain of ZMYND8 directly interacts with PPPLPhi motifs in the NuRD subunit GATAD2A. PMID: 27732854
- Results showed that suppression of GATAD2A attenuated thyroid cancer cell proliferation and colony formation and promoted apoptosis suggesting it as an essential factor in thyroid cancer cell pathogenesis. PMID: 28260108
-
亞細(xì)胞定位:Nucleus speckle.
-
組織特異性:Ubiquitous, both in fetal and adult tissues.
-
數(shù)據(jù)庫(kù)鏈接:
Most popular with customers
-
-
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody
Applications: ELISA, WB, IHC
Species Reactivity: Human
-
-
-
-
-
-