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  4. CHD3 Recombinant Monoclonal Antibody

CHD3 Recombinant Monoclonal Antibody

  • 中文名稱(chēng)：
    CHD3 Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA251108A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of chromodomain helicase DNA binding protein 3 expression in HepG2 cells using chromodomain helicase DNA binding protein 3 antibody. Green, isotype control; red, chromodomain helicase DNA binding protein 3.
    • Immunocytochemical staining of HepG2 cells with Chromodomain helicase DNA binding protein 3 antibody. Nuclei were stained blue with DAPI; Chromodomain helicase DNA binding protein 3 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
    • Immunohistochemistry was performed on paraffin-embedded human ovarian carcinoma using chromodomain helicase DNA binding protein 3 antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
    • Immunohistochemistry was performed on paraffin-embedded mouse kidney using chromodomain helicase DNA binding protein 3 antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
    • Western blotting analysis using chromodomain helicase DNA binding protein 3 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with chromodomain helicase DNA binding protein 3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    CHD3
  • 別名：
    CHD3; Chromodomain Helicase DNA Binding Protein 3; ZFH; Chromodomain-Helicase-DNA-Binding Protein 3; Mi-2 Autoantigen 240 KDa Protein; ATP-Dependent Helicase CHD3; Zinc Finger Helicase; Mi2-ALPHA; Mi-2a; CHD-3; HZFH; Zinc-Finger Helicase (Snf2-Like); EC 3.6.4.12; MI2-ALPHA; Mi2-Alpha; EC 3.6.1; SNIBCPS; MI-2A
  • 反應(yīng)種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human CHD3 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類(lèi)型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    2D2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, FC, ICC, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
    IHC 1:100-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Involved in transcriptional repressiobn as part of the NuRD complex. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
  • 基因功能參考文獻(xiàn)：
    1. The tandem PHD fingers of CHD3 bind histone H3 tails. PMID: 29020631
    2. CHD3 and CHD4 exhibit distinct nuclear localization patterns in unperturbed cells, revealing a subset of specific target genes. PMID: 28977666
    3. Mutations in CHD3 gene is associated with cutaneous T cell lymphoma and Sezary syndrome. PMID: 26551667
    4. CHD3 was confirmed to interact with NES1 in NS2, and a disruption to this interaction by mutation in NES1 significantly delayed viral vRNPs export and viral propagation. PMID: 25213355
    5. CHD3.1 and ACF1-SNF2H display counteractive activities but similar histone affinities. PMID: 25533843
    6. In this study, the chromatin remodeler chromodomain helicase DNA binding (CHD3) protein is identified as an important component of the initial repression of the herpesvirus genome. PMID: 24425734
    7. effect of progressive C-terminal deletions of CHD3, which indicated that sequences required for repression of PS1 lie between amino acids 1955 and 1877 PMID: 17489097
    8. Pericentrin forms complexes with CHD3 and CHD4, but a distinct CHD3-pericentrin complex is required for centrosomal anchoring of pericentrin/gamma-tubulin and for centrosome integrity. PMID: 17626165
    9. UV radiation regulates Mi-2 through protein translation and stability PMID: 18922793

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  • 亞細(xì)胞定位：
    Nucleus, PML body. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    SNF2/RAD54 helicase family
  • 組織特異性：
    Widely expressed.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 1918

    OMIM: 602120

    KEGG: hsa:1107

    STRING: 9606.ENSP00000369716

    UniGene: Hs.25601



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