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  4. CDCA5 Recombinant Monoclonal Antibody

CDCA5 Recombinant Monoclonal Antibody

  • 中文名稱：
    CDCA5 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA436367A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in:Raji whole cell lysate(30μg)
      All lanes: Sororin antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 28 kDa
      Observed band size: 35 kDa
      Exposure time：3min
    • IHC image of CSB-RA436367A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of A549 cell with CSB-RA436367A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-RA436367A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    CDCA5
  • 別名：
    Sororin (Cell division cycle-associated protein 5) (p35), CDCA5
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide from human CDCA5 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    20F9
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.
  • 基因功能參考文獻：
    1. High CDCA5 expression is associated with hepatocellular carcinoma. PMID: 30015982
    2. These results suggest that satellite I RNA plays a role in stabilizing RBMX and Sororin in the ncRNP complex to maintain proper sister chromatid cohesion. PMID: 29383807
    3. Silencing of CDCA5 suppresses proliferation of gastric cancer cells by inducing G1-phase arrest via downregulating CCNE1. PMID: 29326043
    4. Sororin participates in the regulation of centromeric cohesion during meiosis in collaboration with SGO2-PP2A. PMID: 26951638
    5. The results suggest that CDCA5 functions as a critical gene supporting oral squamous cell carcinoma progression and that targeting CDCA5 may be a useful therapeutic strategy for oral squamous cell carcinoma. PMID: 26497678
    6. phosphorylation plays unexpected roles in regulating the subcellular localization of Sororin. PMID: 26177583
    7. the C-terminus of Sororin functions as an anchor binding to SA2, which facilitates other conserved motifs on Sororin to interact with other proteins to regulate sister chromatid cohesion and separation. PMID: 25608232
    8. We propose that the Prp19 complex and the splicing machinery contribute to the establishment of cohesion by promoting Sororin accumulation during S phase, and are, therefore, essential to the maintenance of genome stability. PMID: 25092791
    9. A transcriptome-wide analysis revealed that SNW1 or PRPF8 depletion affects the splicing of specific introns in a subset of pre-mRNAs, including pre-mRNAs encoding the cohesion protein sororin and the APC/C subunit APC2. PMID: 25257309
    10. Targeting p35/Cdk5 signalling via CIP-peptide promotes angiogenesis in hypoxia. PMID: 24098701
    11. Data show that the MEK-ERK pathway regulates cell cycle-related neuronal apoptosis (CRNA) by elevating the levels of cyclin D1, and the increase in cyclin D1 attenuates the activation of cyclin-dependent kinase 5 (cdk5) by its neuronal activator p35. PMID: 22833568
    12. Sororin is phosphorylated by Cdk1/cyclin B at prophase and acts as a docking protein to bring Plk1 into proximity with SA2, resulting in the phosphorylation of SA2 and the removal of cohesin complexes from chromosomal arms. PMID: 21987589
    13. the interaction of the highly conserved motif at the C terminus of sororin with the cohesin complex is critical to its ability to mediate sister chromatid cohesion. PMID: 21115494
    14. Transactivation of CDCA5 and its phosphorylation at Ser209 by ERK play an important role in lung cancer proliferation. PMID: 20551060
    15. data indicate that sororin interacts with chromatin-bound cohesin and functions during the establishment or maintenance of cohesion in S or G2 phase, respectively PMID: 17349791
    16. Sororin protects centromeric cohesion in response to the spindle checkpoint, but prevents the removal of cohesion by a mechanism independent of the anaphase-promoting complex. PMID: 17361102

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  • 亞細胞定位：
    Nucleus. Chromosome. Cytoplasm. Note=Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown.
  • 蛋白家族：
    Sororin family
  • 數據庫鏈接：

    HGNC: 14626

    OMIM: 609374

    KEGG: hsa:113130

    STRING: 9606.ENSP00000275517

    UniGene: Hs.434886



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