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  4. BCL11A Recombinant Monoclonal Antibody

BCL11A Recombinant Monoclonal Antibody

  • 中文名稱：
    BCL11A Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA940706A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Jurkat whole cell lysate(30μg)
      All lanes: BCL 11A antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 91 kDa
      Observed band size: 125 kDa
      Exposure time：1min
    • IHC image of CSB-RA940706A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of SH-SY5Y cell with CSB-RA940706A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA940706A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    B-cell lymphoma/leukemia 11A (BCL-11A) (B-cell CLL/lymphoma 11A) (COUP-TF-interacting protein 1) (Ecotropic viral integration site 9 protein homolog) (EVI-9) (Zinc finger protein 856), BCL11A, CTIP1 EVI9 KIAA1809 ZNF856
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide from human BCL11A protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    2C11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Transcription factor. Associated with the BAF SWI/SNF chromatin remodeling complex. Binds to the 5'-TGACCA-3' sequence motif in regulatory regions of target genes, including a distal promoter of the HBG1 hemoglobin subunit gamma-1 gene. Involved in regulation of the developmental switch from gamma- to beta-globin, probably via direct repression of HBG1; hence indirectly repressing fetal hemoglobin (HbF) level. Involved in brain development. May play a role in hematopoiesis. Essential factor in lymphopoiesis required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of NR2F2.
  • 基因功能參考文獻：
    1. BCL11A interacts with histone methyltransferase (PRC2) and histone deacetylase (NuRD and SIN3A) complexes through their common subunit, RBBP4/7. PMID: 29263092
    2. BCL11A is integral to lung squamous cell carcinoma pathology and highlights the disruption of the BCL11A-SOX2 transcriptional programme as a novel candidate for drug development. PMID: 30127402
    3. High BCL11A and MDR1 expression was associated with a poor response to chemotherapy. PMID: 29469608
    4. miR-146a is a potential tumor suppressor gene in human neuroblastoma via directly targeting BCL11A PMID: 30077189
    5. By perturbing BCL11A-DNMT1 interaction, miR-137 impairs cancer stemness and suppresses tumor development in Triple negative breast cancer. PMID: 29975921
    6. APOL1, alpha-thalassemia, and BCL11A variants as a genetic risk profile for progression of chronic kidney disease in sickle cell anemia PMID: 27658436
    7. the coding mRNA sequence of BCL11A can be targeted by miR-210. PMID: 29186860
    8. BCL11A frameshift mutation associated with dyspraxia and hypotonia affecting the fine, gross, oral, and speech motor systems PMID: 28960836
    9. IGF2BP1 mediates posttranscriptional loss of BCL11A in cultured human adult erythroblasts PMID: 28652347
    10. The expression levels of Bcl11a and Mdm2, Pten in B-ALL patients with CR were decreased significantly when compared with the healthy control (P < 0.05). PMID: 28544358
    11. Studied association of BCL11A single nucleotide polymorphisms(snps) and HBS1L-MYB Intergenic snps with Hereditary Persistence of Fetal Hemoglobin (HPFH) in a cohort of sickle cell patients. PMID: 28332727
    12. both BCL11A and HMIP-2 were associated with increased endogenous levels of HbF. Interestingly, we also found that BCL11A was associated with higher induction of HbF with HU. PMID: 27838552
    13. High BCL11A expression level was correlated with lower complete remission rate and shorter overall survival in adult acute myeloid leukemia patients. PMID: 28164500
    14. Identification of novel mutations in the HbF repressor gene BCL11A in patients with autism and intelligence disabilities. PMID: 28891213
    15. Ubiquitous knockdown of BCL11A in hematopoietic stem and progenitor cells impairs hematopoietic reconstitution after transplantation. PMID: 27599293
    16. BCL11A rs11886868 and rs4671393 genotype variations and correspondingly high BCL11A plasma levels are related to laryngeal squamous cell carcinoma, besides, differences in plasma levels and genotype distribution may be related to lymph node metastasis status and the stage of laryngeal squamous cell carcinoma. PMID: 28225775
    17. BCL11A may operate in transformation of CML from chronic to acute phase in some persons PMID: 27285855
    18. The BCL11A gene was found to be potentially targeted by 12 MicroRNAs that were up-regulated in Hereditary persistence of fetal hemoglobin deletion type-2 (HPFH-2) or in Sicilian deltabeta-thalassemia. A down-regulation of BCL11A gene expression in HPFH-2 was verified by quantitative polymerase chain reaction. PMID: 27591578
    19. The BCL11A protein is highly expressed in breast cancer and knock-down of BCL11A promotes the apoptosis of MDA-MB-231 cells. PMID: 27774950
    20. his study confirms that the T/C variant (rs11886868) of the BCL11A gene causing downregulation of BCL11A gene expression in adult erythroid precursors results in the induction of HbF and ameliorates the severity of a-thalassaemia as well as sickle cell anaemia. PMID: 27377501
    21. report of an intellectual disability syndrome caused by de novo heterozygous missense, nonsense, and frameshift mutations in BCL11A, encoding a transcription factor that is a putative member of the BAF swi/snf chromatin-remodeling complex PMID: 27453576
    22. Reduced rate of sickle-related complications in Brazilian patients carrying HbF-promoting alleles at the BCL11A and HMIP-2 loci PMID: 26888013
    23. the association between modifier loci (beta-globin gene cluster, HBS1L-MYB intergenic region and BCL11A) and Hb F levels in Chinese Zhuang beta-TI patients PMID: 28361591
    24. The combination of the genotypes GG and CT explains more phenotypic variance than the sum of the two BCL11A SNPs taken individually. PMID: 27077760
    25. our study suggests that FOXQ1 regulates prostate cancer cell proliferation and apoptosis by regulating BCL11A/MDM2 expression and indicates that FOXQ1 may serve as a potential therapeutic target for prostate cancer. PMID: 27573292
    26. this study aimed to find the effect of these genetic modifiers, especially in the XmnI locus, rs11886868, rs766432 (BCL11A), and rs9399137 (HBS1L-MYB), among beta thalassemia (beta-thal) and Hemoglobin E/beta-thal patients in Indonesia. PMID: 27009595
    27. The frequency of rs11886868 was determined in Colombian sickle cell anemia patients. It indicated an Amerindian ethnic background. PMID: 26849705
    28. Extensive genetic analyses have validated BCL11A as a potent repressor of fetal hemoglobin level. Studies of BCL11A exemplify how contextual gene regulation may often be the substrate for trait-associated common genetic variation PMID: 26375765
    29. Association has been found between variants at BCL11A erythroid-specific enhancer and fetal hemoglobin levels among sickle cell disease patients in Cameroon. PMID: 26393293
    30. These data suggest a possible role for BCL11A expression in acute myeloid leukaemia biology PMID: 26707798
    31. only HbF inducer efficient in rescuing the ability to differentiate along the erythroid program, even in K562 cell clones expressing high levels of BCL11A-XL, suggesting that BCL11A-XL activity is counteracted by mithramycin. PMID: 26342260
    32. a successful induction of gamma-globin includes a reduction in BCL11A, KLF1 and TAL1 expression. PMID: 26053062
    33. this study found that LRF/ZBTB7A transcription factor occupies fetal gamma-globin genes and maintains the nucleosome density necessary for gamma-globin gene silencing in adults, and that LRF confers its repressive activity through a NuRD repressor complex independent of the fetal globin repressor BCL11A. PMID: 26816381
    34. BCL11A has an important role in triple-negative breast cancer and normal mammary epithelial cells PMID: 25574598
    35. The study compares polymorphism at BCL11A to HBS1L-MYB loci and explains less of the variance in HbF in patients with sickle cell disease in Cameroon. PMID: 25488618
    36. Common HbF BCL11A enhancer haplotypes in patients with African origin and Arab-Indian sickle cell anemia have similar effects on HbF but they do not explain their differences in HbF. PMID: 25703683
    37. the study of these rare patients and orthogonal genetic data demonstrates that BCL11A plays a central role in silencing HbF in humans and implicates BCL11A as an important factor for neurodevelopment PMID: 25938782
    38. SCF-mediated gamma-globin gene expression in adult human erythroid cells is associated with KLF1, BCL11A and SOX6 down-regulation. PMID: 25457384
    39. Genetic variant in the BCL11A (rs1427407), but not HBS1-MYB (rs6934903) loci associate with fetal hemoglobin levels in Indian sickle cell disease patients. PMID: 25457385
    40. Genetic variants of BCL11A is associated with sickle cell disease. PMID: 24667352
    41. HbA2 levels were associated with SNPs in BCL11A, mediated by the association of this locus with gamma-globin gene expression and fetal hemoglobin levels. PMID: 25042611
    42. Twelve SNPs at the BCL11A gene were shown to modify HbF levels. PMID: 24502199
    43. Data suggest that segregation of BCL11A haplotype 2 indicating an involvement of this locus in Hb F expression. PMID: 23777413
    44. The influence of the BCL11A polymorphism on the phenotype of patients with beta thalassemia could be affected by the beta globin locus control region and/or the Xmn1-HBG2 genotypic background. PMID: 23541515
    45. BCL11A overexpression predicts survival and relapse in non-small cell lung cancer and is modulated by microRNA-30a and gene amplification. PMID: 23758992
    46. Genome engineering reveals the enhancer is required in erythroid but not B-lymphoid cells for BCL11A expression. PMID: 24115442
    47. Immunohistochemical staining of mouse brain showed strong expression of BCL11A in the cortical regions and also in the pyramidal cell layers in the CA1 and CA3 regions of the hippocampus. PMID: 23975195
    48. BCL11A contains F/YSXXLXXL/Y motifs that mediate highly selective binding to the LBDs of orphan nuclear receptors NR2E1/TLX, NR2E3/PNR and the NR2F/COUP-TF family. These motifs are required for BCL11A/COUP-TFII-mediated repression of foetal globin genes and a lncRNA termed Bgl3. The motifs are conserved in other Nuclear receptor cofactors such as NSD1, constituting a new signature motif related to LXXLL and the CoRNR box. PMID: 23975195
    49. Regulators, including BCL11A,MYB, and KLF1, hold great promise to develop targeted and more effective approaches for HbF induction PMID: 23209159
    50. BCL11A coordinates the hemoglobin switch and fetal hemoglobin silencing by assembling transcriptional corepressor complexes within the beta-globin cluster. PMID: 23576758

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  • 相關疾病：
    Intellectual developmental disorder with persistence of fetal hemoglobin (IDPFH)
  • 亞細胞定位：
    Cytoplasm. Nucleus. Chromosome.; [Isoform 1]: Nucleus matrix.; [Isoform 2]: Cytoplasm. Nucleus.; [Isoform 3]: Cytoplasm. Nucleus.
  • 組織特異性：
    Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development.; [Isoform 1]: Expressed in feta
  • 數據庫鏈接：

    HGNC: 13221

    OMIM: 142335

    KEGG: hsa:53335

    STRING: 9606.ENSP00000338774

    UniGene: Hs.370549



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